scholarly journals Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Mingmei Lu ◽  
Shuo Jiao ◽  
Enting Gao ◽  
Xiuyong Song ◽  
Zhefei Li ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Sinorhizobium Meliloti ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Content Type ◽  
Resistance Determinants ◽  
Medicago Lupulina ◽  
Legume Symbiosis ◽  
Cu And Zn

ABSTRACT The symbiosis of the highly metal-resistant Sinorhizobium meliloti CCNWSX0020 and Medicago lupulina has been considered an efficient tool for bioremediation of heavy metal-polluted soils. However, the metal resistance mechanisms of S. meliloti CCNWSX00200 have not been elucidated in detail. Here we employed a comparative transcriptome approach to analyze the defense mechanisms of S. meliloti CCNWSX00200 against Cu or Zn exposure. Six highly upregulated transcripts involved in Cu and Zn resistance were identified through deletion mutagenesis, including genes encoding a multicopper oxidase (CueO), an outer membrane protein (Omp), sulfite oxidoreductases (YedYZ), and three hypothetical proteins (a CusA-like protein, a FixH-like protein, and an unknown protein), and the corresponding mutant strains showed various degrees of sensitivity to multiple metals. The Cu-sensitive mutant (ΔcueO) and three mutants that were both Cu and Zn sensitive (ΔyedYZ, ΔcusA-like, and ΔfixH-like) were selected for further study of the effects of these metal resistance determinants on bioremediation. The results showed that inoculation with the ΔcueO mutant severely inhibited infection establishment and nodulation of M. lupulina under Cu stress, while inoculation with the ΔyedYZ and ΔfixH-like mutants decreased just the early infection frequency and nodulation under Cu and Zn stresses. In contrast, inoculation with the ΔcusA-like mutant almost led to loss of the symbiotic capacity of M. lupulina to even grow in uncontaminated soil. Moreover, the antioxidant enzyme activity and metal accumulation in roots of M. lupulina inoculated with all mutants were lower than those with the wild-type strain. These results suggest that heavy metal resistance determinants may promote bioremediation by directly or indirectly influencing formation of the rhizobium-legume symbiosis. IMPORTANCE Rhizobium-legume symbiosis has been promoted as an appropriate tool for bioremediation of heavy metal-contaminated soils. Considering the plant-growth-promoting traits and survival advantage of metal-resistant rhizobia in contaminated environments, more heavy metal-resistant rhizobia and genetically manipulated strains were investigated. In view of the genetic diversity of metal resistance determinants in rhizobia, their effects on phytoremediation by the rhizobium-legume symbiosis must be different and depend on their specific assigned functions. Our work provides a better understanding of the mechanism of heavy metal resistance determinants involved in the rhizobium-legume symbiosis, and in further studies, genetically modified rhizobia harboring effective heavy metal resistance determinants may be engineered for the practical application of rhizobium-legume symbiosis for bioremediation in metal-contaminated soils.

scholarly journals Metagenomics of Antimicrobial and Heavy Metal Resistance in the Cecal Microbiome of Fattening Pigs Raised without Antibiotics

2021 ◽  
Vol 87 (8) ◽  
Author(s):  
Paiboon Tunsagool ◽  
Wuttichai Mhuantong ◽  
Sithichoke Tangphatsornruang ◽  
Nutthee Am-In ◽  
Rungtip Chuanchuen ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Resistance Genes ◽  
Tetracycline Resistance ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Feed Additives ◽  
Farm Animals ◽  
Content Type ◽  
Fattening Pigs ◽  
Cu And Zn

ABSTRACT This study aimed to assess the cecal microbiome, antimicrobial resistance (AMR), and heavy-metal resistance genes (MRGs) in fattening pigs raised under antibiotic-free (ABF) conditions compared with ordinary industrial pigs (control, C) using whole-genome shotgun sequencing. ABF pigs showed enrichment of Prevotella (33%) and Lactobacillus (13%), whereas Escherichia coli (40%), Fusobacterium, and Bacteroides (each at 4%) were notably observed in the C group. Distinct clusters of cecal microbiota of ABF and C pigs were revealed; however, microbiota of some C pigs (C1) appeared in the same cluster as ABF and were totally separated from the remaining C pigs (C2). For AMR genes, the most abundant genes, tet(Q) (35.7%) and mef(A) (12.7%), were markedly observed in the ABF group, whereas tet(Q) (26.2%) and tet(W) (10.4%) were prominently shown in the C group. tet(Q) was positively correlated with Prevotella in ABF and C1 samples. In the C2 group, the prominent tet(W) was positively correlated with Fusobacterium and Bacteroides. The pigs studied here have never received tetracycline, but pregnant sows received chlortetracycline once every 7 days before parturition. Chromosomal Cu and Zn resistance genes were also shown in both groups regardless of the received Cu and Zn feed additives. A higher abundance of multimetal resistance genes was observed in the C group (44%) than with the ABF group (41%). In conclusion, the microbiome clusters in some C pigs were similar to that in ABF pigs. High-abundance tetracycline resistance genes interrelated to major bacteria were observed in both ABF and C pigs. MRGs were also observed. IMPORTANCE AMR is an increasing problem in farm animals, and raising farm animals without antibiotics is one method that could solve this problem. Our study showed that only some tetracycline and macrolide resistance genes, tet(Q), tet(W), and mef(A), were markedly abundant in ABF and C groups. The tet(Q) and tet(W) genes interrelated to different predominant bacteria in each group, showing the potential role of major bacteria as reservoirs of AMR genes. In addition, chromosomal Cu and Zn resistance genes were also observed in both pig groups, independent of the use of Cu and Zn additives on both farms. The association of MRGs and AMR genotypes and phenotypes, together with the method to resensitize bacteria to antibiotics, should be studied further to unveil the cause of genes conferring high-level resistance and solve these problems.

scholarly journals Draft Genome Sequence of Heavy Metal-Resistant Bacillus cereus NWUAB01

2019 ◽  
Vol 8 (7) ◽  
Author(s):  
Olubukola Oluranti Babalola ◽  
Bukola Rhoda Aremu ◽  
Ayansina Segun Ayangbenro
Keyword(s):  
Heavy Metal ◽  
Bacillus Cereus ◽  
Genome Sequence ◽  
Gold Mining ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Mining Site ◽  
Content Type

Bacillus cereus NWUAB01 was isolated from a gold-mining site in Vryburg, South Africa, for its multiple heavy metal resistance properties. Here, we report the draft genome sequence of B. cereus NWUAB01 obtained with Illumina sequencing.

Heavy metal resistance mechanisms in actinobacteria for survival in AMD contaminated soils

Geochemistry ◽  
2005 ◽  
Vol 65 ◽  
pp. 131-144 ◽  
Author(s):  
Andre Schmidt ◽  
Götz Haferburg ◽  
Manuel Sineriz ◽  
Dirk Merten ◽  
Georg Büchel ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Jana Harichová ◽  
Edita Karelová ◽  
Domenico Pangallo ◽  
Peter Ferianc
Keyword(s):  
Heavy Metal ◽  
Soil Sample ◽  
16S Rdna ◽  
Resistance Genes ◽  
Contaminated Soil ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Resistance Determinants ◽  
Cultivable Bacteria ◽  
Rdna Sequences

AbstractIn this study we performed the phylogenetic analysis of non-cultivable bacteria from anthropogenically disturbed soil using partial sequences of the 16S rRNA (16S rDNA) and the heavy-metal resistance genes. This soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and also a trace amount of cadmium (<0.25 mg/kg). The 16S rDNA sequences from a total of 74 bacterial clones were distributed into four broad taxonomic groups, Acidobacteria, Actinobacteria, Bacteroidetes and Gemmatimonadetes, and some of them were unidentified. Comparing our clone sequences with those from the GenBank database, only 9 clones displayed high similarity to known bacteria belongig to actinomycetes; others were identified as uncultured ones. Among clones evidently Actinobacteria predominated. Sixteen clones from soil sample carried only the nccA-like heavy-metal-resistance genes and all sequences showed too low similarity to known proteins encoded by these genes. However, our results suggested that the heavy-metal-contaminated soil is able to present very important reservoir of the new and until now unknown partly bacteria, partly heavy-metal-resistance determinants and their products. Bacteria and nccA-like genes identified in this study could represent the objects of interest as bioremediation agents because they can be potentially used in different transformation and immobilization processes.

scholarly journals Draft Genome Sequence of Arthrobacter oryzae TNBS02, a Bacterium Containing Heavy Metal Resistance Genes, Isolated from Soil of Antarctica

2019 ◽  
Vol 8 (4) ◽  
Author(s):  
Yong-Joon Cho ◽  
Ahnna Cho ◽  
Soon Gyu Hong ◽  
Han-Gu Choi ◽  
Ok-Sun Kim
Keyword(s):  
Heavy Metal ◽  
Genome Sequence ◽  
Resistance Genes ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Terra Nova Bay ◽  
Victoria Land

Arthrobacter oryzae TNBS02 was isolated from soil at Terra Nova Bay of Victoria Land, Antarctica. The genome consists of a chromosome with 4,248,670 bp which contains a total of 3,994 genes.

scholarly journals Genetic Determinants for Cadmium and Arsenic Resistance among Listeria monocytogenes Serotype 4b Isolates from Sporadic Human Listeriosis Patients

2013 ◽  
Vol 79 (7) ◽  
pp. 2471-2476 ◽  
Author(s):  
Sangmi Lee ◽  
M. Rakic-Martinez ◽  
L. M. Graves ◽  
T. J. Ward ◽  
R. M. Siletzky ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Listeria Monocytogenes ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Genetic Determinants ◽  
Chromosomal Locus ◽  
Cadmium Resistance ◽  
Arsenic Resistance ◽  
Content Type ◽  
Serotype 4B

ABSTRACTInListeria monocytogenesserotype 4b isolates from sporadic listeriosis, heavy metal resistance was primarily encountered in certain clonal groups (ECI, ECII, and ECIa). All arsenic-resistant isolates harbored the arsenic resistance cassette previously identified in pLI100; ECIa harbored additional arsenic resistance genes and a novel cadmium resistance determinant in a conserved chromosomal locus.

scholarly journals Draft Genome Sequence of Megaplasmid-Bearing Staphylococcus sciuri Strain B9-58B, Isolated from Retail Pork

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Leena Neyaz ◽  
Anand B. Karki ◽  
Mohamed K. Fakhr
Keyword(s):  
Heavy Metal ◽  
Stress Response ◽  
Antimicrobial Agent ◽  
Genome Sequence ◽  
Draft Genome ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Draft Genome Sequence ◽  
Content Type

Here, we report the genome sequence of the megaplasmid-bearing Staphylococcus sciuri strain B9-58B, isolated from retail pork. This strain contains a 2,761,440-bp chromosome and a 162,858-bp megaplasmid. The genome contains putative genes involved in virulence, the stress response, and antimicrobial agent and heavy metal resistance.

scholarly journals Full-Length Nucleotide Sequences ofmcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins

2016 ◽  
Vol 60 (9) ◽  
pp. 5589-5591 ◽  
Author(s):  
Katrin Zurfluh ◽  
Jochen Klumpp ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan
Keyword(s):  
Escherichia Coli ◽  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Multidrug Resistant ◽  
Metal Resistance ◽  
Content Type ◽  
Esbl Gene ◽  
Extended Spectrum ◽  
Different Origins ◽  
First Time

ABSTRACTHere, we present the full sequences of threemcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producingEscherichia coli. The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containingmcr-1localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL geneblaCTX-M-1, and heavy metal resistance.

scholarly journals Coexistence of Heavy Metal and Antibiotic Resistance within a Novel Composite Staphylococcal Cassette Chromosome in a Staphylococcus haemolyticus Isolate from Bovine Mastitis Milk

2015 ◽  
Vol 59 (9) ◽  
pp. 5788-5792 ◽  
Author(s):  
Huping Xue ◽  
Zhaowei Wu ◽  
Longping Li ◽  
Fan Li ◽  
Yiqing Wang ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Coagulase Negative Staphylococci ◽  
Content Type ◽  
Staphylococcus Haemolyticus ◽  
Staphylococcal Cassette Chromosome

ABSTRACTThe structure of a composite staphylococcal cassette chromosome (SCC) carried by a methicillin-resistantStaphylococcus haemolyticus(NW19A) isolated from a bovine milk sample was analyzed. The formation of the circular forms of both single SCC elements and composite SCC elements was detected in NW19A. Twenty heavy metal and antibiotic resistance-related genes coexisted in this composite SCC, suggesting that these genes might be coselected under environmental pressure. Themecgene complex in NW19A, designated type C3, is different from classic C1 or C2 gene complexes structurally and likely evolves differently. Furthermore, results from alignment of the SCC composite island of NW19A with 50 related sequences from different staphylococcal strains provided additional evidence to support the notion that coagulase-negative staphylococci (CoNS) are the original host of heavy metal resistance genes among staphylococci. Given that a SCC composite island could transfer freely among different staphylococcal species from different hosts, more attention should be paid to contamination with heavy metals and antibiotics in dairy farming environments, including wastewater, soil, feces, and feed.

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