scholarly journals Determination of non-structural protein level for Turkey foot-and-mouth disease vaccine antigens during in-process

2020 ◽  
Vol 9 (2) ◽  
pp. 97
Author(s):  
Beyhan Sareyyüpoğlu ◽  
Can Çokçalışkan ◽  
Aydın Çoskuner ◽  
Veli Gülyaz
Keyword(s):  
Protein Level ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Protein ◽  
Vaccine Antigens ◽  
Foot And Mouth

scholarly journals Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt

2020 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Mariam M. Abd El-Rhman ◽  
Diea G. Abo El-Hassan ◽  
Walid S. Awad ◽  
Sayed A. H. Salem
Keyword(s):  
Solid Phase ◽  
Foot And Mouth Disease ◽  
Elisa Test ◽  
Mouth Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Structural Protein ◽  
Fmdv Serotypes ◽  
Foot And Mouth ◽  
Trivalent Vaccine

Aim: The present study was aimed to investigate the epidemic situation of foot-and-mouth disease (FMD) in Egypt from 2016 to 2018 based on the detection of FMD virus (FMDV) in carrier or previously infected animals, by determination of antibodies against non-structural protein (NSP), implementation a pilot study on circulating FMDV serotypes and assure the efficacy of locally produced inactivated trivalent vaccine. Materials and Methods: A total of 1500 sera were collected from apparent healthy vaccinated cattle and buffaloes from three Egyptian geographical sectors, representing ten governorates. Determination of FMD antibodies against NSP was carried out using 3ABC enzyme-linked immunosorbent assay (ELISA) test. Serotyping of the circulating FMDV and assure the vaccine efficacy was performed using solid-phase competitive ELISA. Results: The 3ABC ELISA test revealed 26.4% and 23.7% positive for FMDV-NSP antibodies in cattle and buffalo sera, respectively. The highest positivity was in Delta Sector among both cattle 42.3% and buffaloes 28.8%. Serotyping of FMDV-positive NSP sera in El-Qalyubia Governorate for the circulating FMDV serotypes O, A, and Southern African Territories (SAT) 2 was 52.2%, 17.4%, and 30.4% in cattle and 31.8%, 27.3%, and 40.9% in buffaloes, respectively. The overall protection level due to the vaccination program was 62.1 and 60.9% in cattle and buffaloes, respectively, while the protective level of the FMDV serotypes O, A, and SAT2 included in the inactivated trivalent vaccine was 73.9, 84.6, and 63.8% in cattle and 72.3, 82.3, and 63.5% in buffaloes, respectively. Conclusion: The present study recommended full determination for the immunogenic relationship between the vaccine strains and the field strains to attain maximum protection against the circulating viruses.

scholarly journals Effect of data quality on estimates of farm infectiousness trends in the UK 2001 foot-and-mouth disease epidemic

2006 ◽  
Vol 4 (13) ◽  
pp. 235-241 ◽  
Author(s):  
Nicholas J Savill ◽  
Darren J Shaw ◽  
Rob Deardon ◽  
Michael J Tildesley ◽  
Matthew J Keeling ◽  
...  
Keyword(s):  
Mathematical Models ◽  
Data Quality ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Inaccurate Data ◽  
Foot And Mouth ◽  
The Uk ◽  
Parameter Values

Most of the mathematical models that were developed to study the UK 2001 foot-and-mouth disease epidemic assumed that the infectiousness of infected premises was constant over their infectious periods. However, there is some controversy over whether this assumption is appropriate. Uncertainty about which farm infected which in 2001 means that the only method to determine if there were trends in farm infectiousness is the fitting of mechanistic mathematical models to the epidemic data. The parameter values that are estimated using this technique, however, may be influenced by missing and inaccurate data. In particular to the UK 2001 epidemic, this includes unreported infectives, inaccurate farm infection dates and unknown farm latent periods. Here, we show that such data degradation prevents successful determination of trends in farm infectiousness.

scholarly journals Foot-and-mouth disease virus non-structural protein 3A modulates cellular innate immune responses to influence host tropism

2020 ◽  
Vol 2 (7A) ◽  
Author(s):  
Soumendu Chakravarti ◽  
Caroline Wright ◽  
Emma Howes ◽  
Richard Kock ◽  
Terry Jackson ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Economic Losses ◽  
Structural Protein ◽  
Host Tropism ◽  
C Terminus ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Species Specific

The picornavirus foot-and-mouth disease virus (FMDV) is responsible for one of the most significant diseases of livestock, leading to large economic losses due to reduced productivity and trade embargoes for areas not certified as disease-free. The picornavirus non-structural protein 3A is involved in replication of the viral RNA genome and is implicated in host tropism of several picornaviruses. Deletions in the C-terminus of 3A have been observed in FMDV outbreaks specific for swine and such viruses are non-pathogenic in cattle. The mechanism for species specific attenuation of FMDV is unknown. We have shown that FMDV containing a C-terminal deletion in 3A is attenuated in bovine cell culture and that the attenuated phenotype can be reversed by the JAK1/2 inhibitor Ruxolitinib (Rux), identifying a role for the induction of interferon stimulated genes (ISGs) in the restricted bovine tropism of the 3A-deleted virus.

scholarly journals Determination of the Effect of Ecthyma Vaccine Virus on Immunity Against the Foot and Mouth Disease (Fmd) Vaccine in Sheep

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Veli Gülyaz ◽  
Fahriye Saraç ◽  
Can Çokçalışkan ◽  
Esra Satır ◽  
Beyhan Sareyyüpoğlu ◽  
...  
Keyword(s):  
Foot And Mouth Disease ◽  
Vaccine Virus ◽  
Mouth Disease ◽  
Foot And Mouth

scholarly journals Early origin and global colonisation of foot-and-mouth disease virus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Pakorn Aiewsakun ◽  
Nakarin Pamornchainavakul ◽  
Chaidate Inchaisri
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Epidemiological Data ◽  
Mouth Disease ◽  
Recent Common Ancestor ◽  
Structural Protein ◽  
Most Recent Common Ancestor ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Early Origin

Abstract In this study, we compiled 84-year worth (1934–2017) of genomic and epidemiological data of foot-and-mouth disease virus (FMDV), and performed comprehensive analyses to determine its early origin and transmission route. We found that recombination is a key feature of FMDV, and that the genomic regions coding for structural and non-structural proteins have markedly different evolutionary histories, and evolve at different rates. Despite all of these differences, analyses of both structural and non-structural protein coding regions consistently suggested that the most recent common ancestor of FMDV could be dated back to the Middle Age, ~ 200 to 300 years earlier than previously thought. The ancestors of the Euro-Asiatic and SAT strains could be dated back to the mid-seventeenth century, and to the mid-fifteenth to mid-sixteenth century, respectively. Our results implicated Mediterranean counties as an early geographical origin of FMDV before spreading to Europe and subsequently to Asia and South America.

scholarly journals Generation of monoclonal antibodies against non-structural protein 3AB of foot-and-mouth disease virus

2012 ◽  
Vol 27 (5) ◽  
pp. 316-319 ◽  
Author(s):  
Tong Lin ◽  
Junjun Shao ◽  
Huiyun Chang ◽  
Shandian Gao ◽  
Guozheng Cong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Protein ◽  
Mouth Disease Virus ◽  
Foot And Mouth
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