PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF CISSUS SETOSA ROXB.

Bambusa arundinacea is a common bamboo species found in Andaman and Nicobar islands. There are over 1200 species of bamboo belonging to 75 genera which has been documented worldwide. Bamboo plants especially the leaves and shoots are known to have cyanogenic glycosides like taxiphyllin and amygdalin apart from vitamin C and other components. Immature bamboo shoots are consumed as vegetable in several asian countries. In the present study the crude extract of Bambusa arundinacea in various solvents was performed. Further preliminary phytochemical screening of the extract was done. Based the results of phytochemical screening, methanol extract was selected for further study. HPLC analysis was done confirm the presence of Amygdalin. GC-MS Analysis of methanol extract of B.arundinacea shows the presence of 40 compounds. The major compounds found in the methanol extracts based on the retension time were beta.-Sitosterol; 2-Methyl-7-phenylindole, 1-(Methylamino)anthraquinone, (R)-(-)-14-Methyl-8-hexadecyn-1-ol and 2-Ethylbutyric acid, nonadecyl ester. Antimicrobial activity of the extracts were tested using two Gram positive bacteria namely S. aureus and pneumonia and two Gram negative bacteria viz. Escherichia coli and Streptococcus mutant. The zone of inhibition was maximum in acetone and ethylacetate extracts while it was minimum for methanol extract. This might be due to the presence of taxiphyllin in the acetone and ethylacetate extract which are relatively non-polar as compared to methanol. To determine whether the inhibition of cell proliferation by methanolic extract from bamboo shoot was due to the induction of apoptosis, PC3 cell exposed to methanol extract were stained with acridine orange/ethidium bromide. The results show that at dose of 150 μg/mL, methanolic extract showed increased percentages of early apoptotic cells. Based on the results of the study, methanol extract of Bambusa arundinacea is a rich source of secondary metabolites, with presence of high levels of flavanoids and tannins. Presence of amygdalin in methanol extract is confirmed by HPLC analysis. Though the extract has a comparatively low antimicrobial activity yet the high anti-apoptotic activity of the extract coupled with a rich antioxidant ativity makes it an ideal candidate as a source of drug for cancer therapy.

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In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don.

Agricultural Science Digest - A Research Journal ◽

10.18805/asd.v37i2.7983 ◽

2017 ◽

Vol 37 (2) ◽

Author(s):

Manish Kapoor ◽

Jyoti Rani ◽

Rupinder Kaur

Keyword(s):

Antimicrobial Activity ◽

Petroleum Ether ◽

Mycobacterium Smegmatis ◽

Cardiac Glycosides ◽

Methanolic Extract ◽

Phytochemical Analysis ◽

Phytochemical Screening ◽

Bacterial Strains ◽

Aqueous Methanol

Present study aims to investigate phytochemical analysis as well as in vitro antimicrobial activity of crude aqueous, methanol, ethanol, chloroform and petroleum ether extracts from leaves of C. roseus. Antimicrobial activity of extract was studied against various bacterial strains (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium smegmatis). Qualitative preliminary phytochemical analysis revealed that alkaloids, phenols, flavonoids, amino acid and cardiac glycosides are present in the extracts. The result for total phenol and flavonoids content was the highest in methanol and the lowest in petroleum ether crude extract. The study revealed that inhibition significantly depend upon the solvent used for extraction and bacterial strain tested for susceptibility. Aqueous extracts were found less efficient as compared to organic solvent. Methanolic extract were found more effective against tested microbes.

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Phytochemical screening and in vitro antimicrobial activity of Typha angustifolia Linn leaves extract against pathogenic gram negative micro organisms

Journal of Pharmacy Research ◽

10.1016/j.jopr.2013.02.010 ◽

2013 ◽

Vol 6 (2) ◽

pp. 280-283 ◽

Cited By ~ 5

Author(s):

Ramesh L. Londonkar ◽

Umesh Madire Kattegouga ◽

Kirankumar Shivsharanappa ◽

Jayashree V. Hanchinalmath

Keyword(s):

Antimicrobial Activity ◽

Typha Angustifolia ◽

Phytochemical Screening ◽

Gram Negative ◽

In Vitro Antimicrobial Activity ◽

Micro Organisms

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Phytochemical screening and in vitro antimicrobial activity of Thymus lanceolatus Desf. from Algeria

Asian Pacific Journal of Tropical Disease ◽

10.1016/s2222-1808(13)60100-0 ◽

2013 ◽

Vol 3 (6) ◽

pp. 454-459 ◽

Cited By ~ 10

Author(s):

Fethi Benbelaïd ◽

Abdelmounaim Khadir ◽

Mohamed Amine Abdoune ◽

Mourad Bendahou

Keyword(s):

Antimicrobial Activity ◽

Phytochemical Screening ◽

In Vitro Antimicrobial Activity

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Antimicrobial Evaluation of Caesalpinia decapetala

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/phyto.v9i12.11185 ◽

2018 ◽

Vol 9 (12) ◽

Author(s):

Sharma V ◽

Lobo R. ◽

Singh G. ◽

Chanana V. ◽

Kalsi V. ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methanolic Extract ◽

Agar Medium ◽

Dilution Method ◽

Phytochemical Screening ◽

In Vivo Evaluation ◽

Crude Drug ◽

Activity Evaluation

Objective: The present work is an attempt to assess the in vitro antimicrobial activity of the leaves of Caesalpinia decapetala (Roth) fabaceae family collected from forest area of Tamilnadu, India. Methods: The crude drug was successively extracted by Soxhlet assembly using Petroleum ether, dichloromethane, ethyl acetate and methanol as solvents. Preliminary phytochemical screening of different extracts was carried out using several colour and precipitative chemical reagents as per described methods. Antimicrobial activity of the extracts was evaluated against fungal strains (Aspergillus fumigatus and Candida albicans), Gram +ve bacteria (Staphylococcus aureus and Streptococcus pyogenes) and Gram –ve bacteria (Escherichia coli and Pseudomonas aeruginosa) using agar wells dilution method. Nutrient agar medium at37 oC and sabouraud dextrose e agar medium at 28 oC were used in antimicrobial activity evaluation and antifungal activity evaluation respectively. Results: Preliminary phytochemical screening of C. decepetala leaves showed the presence of alkaloids, glycosides, phenols, phytosterols, saponins and flavonoids crude drug. C. decapetala leaf extracts exhibited marked dose dependent antibacterial activity in vitro against tested bacteria. Methanolic extract was found to be more potent particularly against Staphylococcus aureus (Gram +ve bacteria) and staphylococcus aeruginosa (Gram -ve bacteria). Conclusion: Various phytochemicals were found to be present in C. decepetala leaves. Methanolic extract of C. decepetala leaves exhibited better antimicrobial activity in vitro and can be used as a good therapeutic approach for infectious disease management and therapy. Further studies on isolation of phyto-constituents and both in vitro and in vivo evaluation of pharmacological activities of isolated bioactive constituents of the crude drug are recommended as future works.

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