In-Vitro Study of Antimicrobial and Antiproliferative activity of Cyanogenic glycoside extracted from Bamboo shoot of Bambusa arundinacea

2021 ◽  
pp. 5661-5667
Author(s):  
Binku Mondal
Keyword(s):  
Antimicrobial Activity ◽  
Hplc Analysis ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
In Vitro Study ◽  
Bamboo Shoot ◽  
Phytochemical Screening ◽  
Bamboo Shoots ◽  
Apoptotic Activity

Bambusa arundinacea is a common bamboo species found in Andaman and Nicobar islands. There are over 1200 species of bamboo belonging to 75 genera which has been documented worldwide. Bamboo plants especially the leaves and shoots are known to have cyanogenic glycosides like taxiphyllin and amygdalin apart from vitamin C and other components. Immature bamboo shoots are consumed as vegetable in several asian countries. In the present study the crude extract of Bambusa arundinacea in various solvents was performed. Further preliminary phytochemical screening of the extract was done. Based the results of phytochemical screening, methanol extract was selected for further study. HPLC analysis was done confirm the presence of Amygdalin. GC-MS Analysis of methanol extract of B.arundinacea shows the presence of 40 compounds. The major compounds found in the methanol extracts based on the retension time were beta.-Sitosterol; 2-Methyl-7-phenylindole, 1-(Methylamino)anthraquinone, (R)-(-)-14-Methyl-8-hexadecyn-1-ol and 2-Ethylbutyric acid, nonadecyl ester. Antimicrobial activity of the extracts were tested using two Gram positive bacteria namely S. aureus and pneumonia and two Gram negative bacteria viz. Escherichia coli and Streptococcus mutant. The zone of inhibition was maximum in acetone and ethylacetate extracts while it was minimum for methanol extract. This might be due to the presence of taxiphyllin in the acetone and ethylacetate extract which are relatively non-polar as compared to methanol. To determine whether the inhibition of cell proliferation by methanolic extract from bamboo shoot was due to the induction of apoptosis, PC3 cell exposed to methanol extract were stained with acridine orange/ethidium bromide. The results show that at dose of 150 μg/mL, methanolic extract showed increased percentages of early apoptotic cells. Based on the results of the study, methanol extract of Bambusa arundinacea is a rich source of secondary metabolites, with presence of high levels of flavanoids and tannins. Presence of amygdalin in methanol extract is confirmed by HPLC analysis. Though the extract has a comparatively low antimicrobial activity yet the high anti-apoptotic activity of the extract coupled with a rich antioxidant ativity makes it an ideal candidate as a source of drug for cancer therapy.

In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don.

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Manish Kapoor ◽  
Jyoti Rani ◽  
Rupinder Kaur
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Mycobacterium Smegmatis ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Aqueous Methanol

Present study aims to investigate phytochemical analysis as well as in vitro antimicrobial activity of crude aqueous, methanol, ethanol, chloroform and petroleum ether extracts from leaves of C. roseus. Antimicrobial activity of extract was studied against various bacterial strains (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium smegmatis). Qualitative preliminary phytochemical analysis revealed that alkaloids, phenols, flavonoids, amino acid and cardiac glycosides are present in the extracts. The result for total phenol and flavonoids content was the highest in methanol and the lowest in petroleum ether crude extract. The study revealed that inhibition significantly depend upon the solvent used for extraction and bacterial strain tested for susceptibility. Aqueous extracts were found less efficient as compared to organic solvent. Methanolic extract were found more effective against tested microbes.

A COMPARATIVE STUDY OF IN VITRO ANTIMICROBIAL ACTIVITY AND TLC STUDIES OF PETALS OF SELECTED INDIAN MEDICINAL PLANTS

2016 ◽  
Vol 9 (5) ◽  
pp. 259 ◽  
Author(s):  
Sumathy Rengarajan ◽  
Vijayalakshmi Melanathuru ◽  
Deecaraman Munuswamy ◽  
Sankaranarayanan Sundaram ◽  
Saravanan Thiruverkadu Selvaraj
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Methanol Extract ◽  
Phytochemical Screening ◽  
Layer Chromatography ◽  
Indian Medicinal Plants

ABSTRACTObjective: The present study was to evaluate the in vitro antibacterial activity, and thin-layer chromatography (TLC) studies from the petals of fourdifferent Indian medicinal plants (Punica granatum, Hibiscus rosa-sinensis, Cassia auriculata, and Moringa oleifera).Methods: The phytochemical screening of the methanol extract of petals of four different Indian medicinal plants was performed using standardprocedures. The antimicrobial activity was tested against various test organisms using the agar disc diffusion method.Results: The preliminary phytochemical screening for petals of four different medicinal plants revealed the presence of flavonoids, alkaloids, tannins,and saponins. From the above study, the results indicated that the methanol extract of M. oleifera petals showed the highest antimicrobial activityagainst Staphylococcus aureus and Bacillus subtilis with zone of inhibition 17.93 and 23.40, respectively, at the concentration of 20 µl/ml and alsoshowed the maximum inhibitory activity at the highest concentration (20 µl/ml) than the lowest concentration (5 µl/ml) against Gram-negativebacteria such as Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, and Gram-positive B. subtilis and S. aureus. TLC studies of methanolextracts of petals of Indian medicinal plants revealed the presence of different phytoconstituents as evidenced by separated compounds with differentRf values.Conclusion: The results obtained in the present study indicate that the petals of four different Indian medicinal plants showed the highest antibacterialactivity and can be used as an antibacterial agent against bacterial diseases.Keywords: Phytochemicals, Antibacterial activity, Thin-layer chromatography.

scholarly journals Antimicrobial Evaluation of Caesalpinia decapetala

2018 ◽  
Vol 9 (12) ◽  
Author(s):  
Sharma V ◽  
Lobo R. ◽  
Singh G. ◽  
Chanana V. ◽  
Kalsi V. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Agar Medium ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
In Vivo Evaluation ◽  
Crude Drug ◽  
Activity Evaluation

Objective: The present work is an attempt to assess the in vitro antimicrobial activity of the leaves of Caesalpinia decapetala (Roth) fabaceae family collected from forest area of Tamilnadu, India. Methods: The crude drug was successively extracted by Soxhlet assembly using Petroleum ether, dichloromethane, ethyl acetate and methanol as solvents. Preliminary phytochemical screening of different extracts was carried out using several colour and precipitative chemical reagents as per described methods. Antimicrobial activity of the extracts was evaluated against fungal strains (Aspergillus fumigatus and Candida albicans), Gram +ve bacteria (Staphylococcus aureus and Streptococcus pyogenes) and Gram –ve bacteria (Escherichia coli and Pseudomonas aeruginosa) using agar wells dilution method. Nutrient agar medium at37 oC and sabouraud dextrose e agar medium at 28 oC were used in antimicrobial activity evaluation and antifungal activity evaluation respectively. Results: Preliminary phytochemical screening of C. decepetala leaves showed the presence of alkaloids, glycosides, phenols, phytosterols, saponins and flavonoids crude drug. C. decapetala leaf extracts exhibited marked dose dependent antibacterial activity in vitro against tested bacteria. Methanolic extract was found to be more potent particularly against Staphylococcus aureus (Gram +ve bacteria) and staphylococcus aeruginosa (Gram -ve bacteria). Conclusion: Various phytochemicals were found to be present in C. decepetala leaves. Methanolic extract of C. decepetala leaves exhibited better antimicrobial activity in vitro and can be used as a good therapeutic approach for infectious disease management and therapy. Further studies on isolation of phyto-constituents and both in vitro and in vivo evaluation of pharmacological activities of isolated bioactive constituents of the crude drug are recommended as future works.

scholarly journals Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 59
Author(s):  
Shanmuga Priya Ramasamy ◽  
Anitha Rajendran ◽  
Muthukrishnan Pallikondaperumal ◽  
Priya Sundararajan ◽  
Fohad Mabood Husain ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Potential Candidate ◽  
Simarouba Glauca

The current study aimed to screen the preliminary phytochemicals in the leaf extract of the medicinal plant Simarouba glauca and to analyze its potential antimicrobial, antioxidant and anticancer properties. The phytochemical profile of the methanol extract was analyzed, and bioactive compounds were identified using chromatography, FTIR and GCMS. Antimicrobial activity and Minimum Inhibitory Concentration (MIC) were determined against 14 bacterial and 6 fungal strains. Moreover, the synergistic effect of a plant extract with commercially available antibiotics was also evaluated using the checkerboard method. The ethanolic and methanolic extracts showed exclusive activity against S. aureus and profound activity against E. coli and S. marcescens. Upon comparing breakpoints, methanolic extract demonstrated higher antimicrobial activity with a MIC value of 3.2 mg/mL against the test pathogens. Furthermore, the extracts demonstrated potential antioxidant activity; methanol extract had higher antioxidant potential compared to the ethanol extract. The major proactive bioactive compound with maximum antioxidant capacity was observed to be terpenoids. The methanol extract of S. glauca showed significant cytotoxicity against the MCF-7 breast cancer cell line with an IC50 value of 16.12 µg/mL. The overall results of our work provide significant evidence for the usage of methanolic extract of S. glauca as an efficient ethnomedicinal agent and a potential candidate for relieving many human ailments.

The Comparative Study of Equisetum pratense, E. sylvaticum, E. telmateia: Accumulation of Silicon, Antioxidant and Antimicrobial Screening

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Methanol Extract ◽  
Previous Treatment ◽  
Total Phenolic ◽  
Quantitative Chemical Analysis ◽  
Qualitative And Quantitative ◽  
Antioxidant And Antimicrobial Activity ◽  
The Comparative Study

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).

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