Evaluation of Population Structure and Genetic Diversity of Cirrhinus mrigala among the Different Freshwater Rivers of Pakistan Using Molecular Marker: Study Implications and Its Importance in Aquaculture

Cirrhinus mrigala (Family Cyprinidae) have vast presence in different studied rivers of Pakistan. Present study was designed to formulate the genetic diversity of desired sites in Cirrhinus mrigala populations using nine Random amplified polymorphic DNA markers. Total hundred individuals were used to conduct this study and were collected from three distinct rivers (River Indus, Chenab and Ravi) with four specified sites with wild of Pakistan were used to study. A random Amplified Polymorphic DNA primer analyzes various bands of polymorphic loci. Mean genetic diversity within and between populations found 81.53% and 2.27% respectively by using analysis of molecular variance. Fixation index was found variably and Pairwise Fst analysis showed significant difference between different sites of studied populations. Genetic divergence information would mark essential in order to improve genetic programming its management and conservation of natural inhabitation. In order to quantify the study of population divergence by using these molecular markers is an efficient way to pin point the genetic diversity within and among population.

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Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.25798 ◽

2018 ◽

Vol 22 (1) ◽

pp. 22

Author(s):

Jayusman Jayusman ◽

Muhammad Na’iem ◽

Sapto Indrioko ◽

Eko Bhakti Hardiyanto ◽

ILG Nurcahyaningsih

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Management Strategies ◽

Genetic Distances ◽

Sampled Data ◽

Toona Sinensis ◽

The Mean ◽

Individual Trees

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

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Distribution of Mating Types and Genetic Diversity of Ascochyta rabiei Populations in Tunisia Revealed by Mating-type-specific PCR and Random Amplified Polymorphic DNA Markers

Journal of Phytopathology ◽

10.1111/j.1439-0434.2007.01285.x ◽

2007 ◽

Vol 155 (10) ◽

pp. 596-605 ◽

Cited By ~ 7

Author(s):

A. Rhaiem ◽

M. Cherif ◽

P. S. Dyer ◽

T. L. Peever

Keyword(s):

Genetic Diversity ◽

Mating Type ◽

Dna Markers ◽

Random Amplified Polymorphic Dna ◽

Ascochyta Rabiei ◽

Mating Types ◽

Specific Pcr

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Genetic diversity of gabiroba based on random amplified polymorphic DNA markers and morphological characteristics

Genetics and Molecular Research ◽

10.4238/2013.march.11.7 ◽

2013 ◽

Vol 12 (3) ◽

pp. 3500-3509 ◽

Cited By ~ 3

Author(s):

E.S. de Assis ◽

E.F. dos Reis ◽

J.F.N. Pinto ◽

L.A.S. Contim ◽

L.A.S. Dias

Keyword(s):

Genetic Diversity ◽

Dna Markers ◽

Random Amplified Polymorphic Dna ◽

Morphological Characteristics

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Use of Random Amplified Polymorphic DNA Markers for Cultivar Identification in Mint

HortScience ◽

10.21273/hortsci.36.4.761 ◽

2001 ◽

Vol 36 (4) ◽

pp. 761-764 ◽

Cited By ~ 15

Author(s):

A.L. Fenwick ◽

S.M. Ward

Keyword(s):

United States ◽

Genetic Diversity ◽

Dna Markers ◽

Geographical Origin ◽

Cultivar Identification ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

The United States ◽

Randomly Amplified Polymorphic Dna ◽

Commercial Oil

Seventeen mint accessions representing the three species grown for commercial oil production in the United States were characterized using randomly amplified polymorphic DNA (RAPD) analysis. The RAPD profiles readily identified the different Mentha species; calculation of genetic distance, based on the number of shared bands, indicated that M. spicata L. is more closely related to M. × gracilis than to M. × piperita. The RAPD profiles also distinguished among eight peppermint accessions of different geographical origin. However, only limited polymorphism was observed among the most widely grown peppermint and Scotch spearmint cultivars. These results indicate a potential lack of genetic diversity in mint cultivars grown for oil in the United States.

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