Morpho-phenological Variability of Flowers Traits and Hybridization of Five Inbred Lines of Cowpea [Vigna unguiculata (L.) Walp.] in Côte d'Ivoire

Author(s):  
Assouman Jean Simon Konan ◽  
Diarrassouba Nafan ◽  
Yao Saraka Didier Martial ◽  
Ossey Aristide Randolphe

Knowledge of the morphology and floral phenology and the mastery of a manual pollination technique in a plant species are essential for its genetic improvement. The objective of this work will be to determine the morpho-phenological characteristics of the flowers of five self-fertilization lines of cowpea and to identify an indicated manual pollination technique. The study was carried out in the Botanical Garden of University Peleforo GON COULIBALY, Côte d’Ivoire where 10 morpho-phenological characters of the flower were evaluated on five lines of cowpea self-fertilization. Thus, three manual pollination techniques (A, B and C) were tested by evaluating traits such as knotting rates, filling rates and maternal and paternal effects on fruit yields. The results revealed four stages of flower development in cowpea. These are successively stage of “floral button initiation”, “dark green floral button”, “pale green or pale-yellow floral button” and “blooming flower”. Of the three manual pollination techniques tested, technique C resulted in higher rates of knotting (45.38%) and pod filling (58.03%). Results also showed significant maternal and xenia effects on fruit yields in the cowpea. On the basis of the results generated on the floral biology of the cowpea, it appears that a hybridization program can now be conducted at the UPGC Botanical Garden for the creation of high-performance varieties adapted to climate change for the benefit of producers in Côte d’Ivoire.

2020 ◽  
pp. 91-97
Author(s):  
AKE ASSI Yolande Epouse DATTE ◽  
GNONSORO Urbain Paul ◽  
KOUAKOU Konan Jean-Marie ◽  
KPAN KPAN Kouakou Gains ◽  
BIÉGO Godi Henri ◽  
...  

The determination of pesticides in food products is an essential issue to guarantee food safety and minimize health risks of consumers. In this study, 30 pineapple juice samples were collected randomly from 30 seller through the city of Abidjan (Côte d’Ivoire) and analyzed for the determination of pesticide residues. The aim of the work was to quantify 30 commonly used pesticides (Metolachlor, Chlorpropham, Parathion-methyl, Chlorfenvinphos, Vinclozolin, Parathion-ethyl, Fenuron, Aldicarb, Metoxuron, Monuron, Methabenzthiazuron, Chlortoluron, Monolinuron, Isoproturon, Diuron, Metobromuron, Metazachlor, Buturon, Linuron, Prometryn, Terbutryn, Desisopropylatrazine, Desethylatrazine, Simazine, Cyanazine, Atrazine, Propazine, Terbuthylazine, Metamitron, Crimidine and Metolachlor) in real samples of pineapple juice. The method used for the determination of these analytes in the complex matrices was high-performance liquid chromatography with UV/Visible detector. Results obtained indicate that 30% of the investigated pineapple juices samples are free of pesticides residues or have a level below Limit Of Detection......


Author(s):  
Sira Bamba ◽  
Henri Marius Godi Biego ◽  
Adama Coulibaly ◽  
Nyamien B. Yves ◽  
Sidibe Daouda

Aims: The aim of this work is to assess the level of aflatoxins contamination in maize produced in five regions (Poro, Hambol, Gontougo, Gbêkê, Indénié-Djuablin) of Côte d'Ivoire. Place and duration of study: In this study 375 samples of maize (grains, cobs, spathes) were taken from February 2016 to January 2017 and the aflatoxin analyses were carried out at the Biotechnology Laboratory, Agriculture and Development of Biological Resources of the Félix Houphouët-Boigny University. Methodology: The aflatoxins (B1, B2, G1 and G2) were extracted and assayed by High Performance Liquid Chromatography (HPLC) according to the AOAC method. Results: The results indicate the presence of aflatoxins B1, B2, G1 and G2 in all forms of maize (grains, cobs, spathes) and in the five regions chosen for the study. The mean concentrations of aflatoxins B1 (AFB1) and the total aflatoxins (AFT) varied from 0.79 ± 0.04 µg/kg to 130.31 ± 22.56 µg/kg and from 2.63 ± 2,35 µg/kg to 169.13 ± 40.39 µg/kg respectively. samples from Indénié-Djuablin, Hambol and Gountougo regions showed the highest proportions of non-compliance with the european Union limit of 5 μg/kg and 10 μg/kg. Regarding AFB1, these proportions vary from 0% to 46% in the regions of Gbêkê, Poro and Hambol, while they are between 54% and 96% in the regions of Indénié-Djuablin and Gontougo. For total aflatoxins, the proportions of non-compliant samples were between 0% and 40% (Gbêkê and Poro), 12% and 56% (Hambol), 56% and 96% (Indénié-Djuablin and Gontougo). Conclusion: These results demonstrate a need for monitoring of maize production by stakeholders in the sector who should adopt good agricultural practices.


2020 ◽  
Vol 152 ◽  
pp. 15605-15620
Author(s):  
Alix Amenan Djoué ◽  
N’Golo Abdoulaye Koné ◽  
Koua Serge Béranger N’Goran ◽  
Louyounan Linda ◽  
Patricia Vanié-Léabo ◽  
...  

Objective: Macrofungi are essential to the structure, functioning and dynamics of ecosystems. In Côte d'Ivoire, studies on macrofungi and the impact of habitats in “Centre National de Floristique (CNF)” on macrofungi diversity are little explored. This study aims to assess macrofungi diversity found in some habitats in the botanical garden. Methodology and results: A mycological inventory was carried out in three habitat types that differ in the level of anthropisation. The surveys were conducted from 2016 to 2018 during rainy seasons, from MayJuly and October- November and sporophores of 151 morpho-species were collected. They belonged to 5 classes, 12 orders, 26 families and 33 genera. The most abundant species belonging to the Agaricaceae (19.87%) followed by Marasmiaceae (10.60%) and Polyporaceae (9.27%) families. The dominant ecological groups were saprotrophic fungi (82.00%). Specific richness was more important in the least visited habitat with 70 species inventoried, followed by the non-visited and the most visited habitat with 54 and 50 species respectively. Conclusion and application of results: This first investigation on macrofungi showed that CNF is rich in saprotrophic fungi belonging at the Agaricaceae, Marasmiaceae and Polyporaceae family. These saprotrophic fungi have the ecological importance in the forest ecosystem management and conservation. Ours finding constitute a database for the future studies on the mycoflora of the botanical garden. These initial data show the importance of botanical garden in the conservation and biodiversity of mycological resources. Keywords: Macrofungi, Diversity, Botanical garden, Centre National de Floristique (CNF), Côte d'Ivoire


2021 ◽  
Vol 15 (1) ◽  
pp. e0009038
Author(s):  
Moritz Seitzer ◽  
Sylvia Klapper ◽  
Humphrey D. Mazigo ◽  
Ulrike Holzgrabe ◽  
Andreas Mueller

Background Even though the international combat against Neglected Tropical Diseases such as schistosomiasis or soil-transmitted helminthiases depends on reliable therapeutics, anthelminthic pharmacovigilance has been neglected on many national African drug markets. Therefore, quality and composition of Albendazole, Mebendazole and Praziquantel locally collected in Burkina Faso, Côte d’Ivoire, Ghana and Tanzania were analysed. Methods Samples of 88 different batches were obtained from randomly selected facilities. Sampling took place in Northwest Tanzania, Western Burkina Faso, Southeast Côte d’Ivoire and Southwest Ghana. Visual examination of both packaging and samples was performed according to the WHO ‘Be Aware’ tool. Products were then screened with the GPHF Minilab, consisting of tests of mass uniformity, disintegration times and thin-layer chromatography (TLC). Confirmatory tests were performed according to international pharmacopoeiae, applying assays for dissolution profiles and high-performance liquid chromatography (HPLC). Findings Despite minor irregularities, appearance of the products did not hint at falsified medicines. However, 19.6% of the brands collected in Ghana and Tanzania were not officially licensed for sale. Mass uniformity was confirmed in 53 out of 58 brands of tablets. 41 out of 56 products passed disintegration times; 10 out of the 15 failing products did not disintegrate at all. Evaluating TLC results, only 4 out of 83 batches narrowly missed specification limits, 18 batches slightly exceeded them. Not more than 46.3% (31 / 67) of the tablets assayed passed the respective pharmaceutical criteria for dissolution. HPLC findings confirmed TLC results despite shifted specification limits: 10 out of 83 tested batches contained less than 90%, none exceeded 110%. Conclusion In the four study countries, no falsified anthelminthic medicine was encountered. The active pharmaceutical ingredient was not found to either exceed or fall below specification limits. Galenic characteristics however, especially dissolution profiles, revealed great deficits.


2019 ◽  
Vol 13 (4) ◽  
pp. 369-374
Author(s):  
A. Yao ◽  
A. Hué ◽  
J. Danho ◽  
P. Koffi-Dago ◽  
M. Sanogo ◽  
...  

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