scholarly journals Short-term Storage of Seeds and Cryopreservation of Embryonic Axes of Lepisanthes fruticosa

2021 ◽  
pp. 112-123
Author(s):  
B. Suryanti ◽  
M. A. Mohd Shukri ◽  
U. R. Sinniah ◽  
S. Nur Atisha ◽  
A. K. Abdul Muhaimin
Keyword(s):  
Liquid Nitrogen ◽  
Research Centre ◽  
Short Term ◽  
Embryonic Axes ◽  
Randomized Design ◽  
Short Term Storage ◽  
Completely Randomized Design ◽  
Vitrification Solution ◽  
Term Storage

Aims: This work highlights short-term storage of recalcitrant Lepisanthes fruticosa seeds and long-term conservation attempts of its embryonic axes (EAs) through cryopreservation. Study design: This study adopted the Completely Randomized Design (CRD). Ten samples were used for each experiment and replicated for 3 – 5 times. Place and Duration of Study: Cryopreservation Laboratory, Agrobiodiversity and Environment Research Centre, MARDI Headquarters, Malaysia, in 2017 and 2018. Methodology: Short-term storage was carried out using fresh seeds at 54% moisture content and stored at 8±1 °C and 25±2 °C for 7 weeks. Three variations to sterilization were attempted to optimize survival while keeping contamination low. Cryopreservation using two different methods were tested, namely vitrification and the encapsulation vitrification method. Vitrification technique involved the pre-culturing of EAs overnight in different sucrose pre-culture concentrations (0, 0.2, 0.4 and 0.6 M) prior to, loading, dehydration with vitrification solution (PVS2), rapid immersion into liquid nitrogen (-196°C), rapid warming, unloading and recovery. While, encapsulation vitrification involved encapsulation of the EAs using 3% sodium alginate followed by exposure to different duration (0, 10, 20, 30, 40 and 50 minutes) of vitrification solution (PVS2) prior to cryopreservation. Results: L. fruticosa seeds can be safely stored for short-term up to 7 weeks of storage either at 8±1 °C or 25±2 °C with no loss in germination. This study also showed that EA was amenable to cryopreservation and 13.33 – 66.67% of viability was obtained when the EAs were cryopreserved using the vitrification technique. The best result was obtained with 66.67% viability, when the EAs were pre-cultured with 0.4M sucrose prior to exposure to PVS2 and liquid nitrogen. Cryopreservation of EAs using the encapsulation-vitrification method was unsuccessful. Conclusion: Seeds of L. fruticosa can be stored for short-term (up to 7 weeks) using hydrated/non-dried seeds where they can be successfully stored at 8±1 °C and 25±2 °C for up to 7 weeks. For long-term (cryopreservation), EAs can be cryopreserved upon pre-culture with 0.4M sucrose prior to exposure to PVS2 and liquid nitrogen through vitrification technique.

Short-term storage of seeds and cryopreservation of embryonic axes of Lepisanthes fruticosa

2021 ◽  
Author(s):  
Suryanti Bustam ◽  
Mohd Shukri Mat Ali ◽  
Uma Rani Sinniah ◽  
Nur Atisha Shamsuddin ◽  
Abdul Muhaimin Abdul Kadir
Keyword(s):  
Moisture Content ◽  
Sodium Alginate ◽  
Liquid Nitrogen ◽  
Short Term ◽  
Embryonic Axes ◽  
Short Term Storage ◽  
Vitrification Solution ◽  
Term Storage

Abstract This work highlights short-term storage of recalcitrant Lepisanthes fruticosa seeds and long-term conservation attempts of its embryonic axes (EAs) through cryopreservation. Short-term storage was carried out using fresh seeds at 54 % moisture content and stored at 8 ±1 °C and 25 ±2 °C for 7 weeks. Three variations to sterilization were attempted to optimize survival while keeping contamination low for cryopreservation. Cryopreservation using two different methods were tested, namely vitrification and the encapsulation vitrification method. Vitrification technique involved the pre-culturing of EAs overnight in different sucrose pre-culture concentrations (0, 0.2, 0.4 and 0.6 M) prior to, loading, dehydration with plant vitrification solution (PVS2), rapid immersion into liquid nitrogen (-196 °C), rapid warming, unloading and recovery. While, encapsulation vitrification involved encapsulation of the EAs using 3 % sodium alginate followed by exposure to different duration (0, 10, 20, 30, 40 and 50 minutes) of PVS2 prior to cryopreservation. L. fruticosa seeds can be safely stored for short-term with no loss in germination up to 7 weeks of storage either at 8 ±1 °C or 25 ±2 °C. This study also showed that EA of L. fruticosa was amenable to cryopreservation, 13.0 – 66.67% of viability was obtained when the EAs were cryopreserved using the vitrification technique while the best result was obtained (66.67 % viability) when the EAs were pre-cultured with 0.4 M sucrose prior to exposure to PVS2 and liquid nitrogen. Cryopreservation of EAs using the encapsulation-vitrification method was unsuccessful.

scholarly journals Management of Apple Maturity and Postharvest Storage Conditions to Increase Polyphenols in Cider

HortScience ◽  
2019 ◽  
Vol 54 (1) ◽  
pp. 143-148 ◽  
Author(s):  
Brianna L. Ewing ◽  
Gregory M. Peck ◽  
Sihui Ma ◽  
Andrew P. Neilson ◽  
Amanda C. Stewart
Keyword(s):  
The United States ◽  
Storage Conditions ◽  
Short Term ◽  
Postharvest Storage ◽  
Total Polyphenols ◽  
Fruit Storage ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

Hard cider production in the United States has increased dramatically during the past decade, but there is little information on how harvest and postharvest practices affect the chemistry of the resulting cider, including concentrations of organoleptically important flavanols. For 2 years we assessed fruit, juice, and cider from a total of five apple (Malus ×domestica Borkh.) cultivars in two experiments: sequential harvests and postharvest storage. Different cultivars were used in 2015 and 2016 with the exception of ‘Dabinett’, which was assessed in both years. There were no differences in polyphenol concentrations in cider made from fruit that was harvested on three separate occasions over a 4-week period in either 2015 or 2016. Fruit storage durations and temperatures had little influence on the chemistry when the experiment was conducted in 2015, but polyphenol concentration was greater after storage in the 2016 experiment. In 2016, total polyphenols in ‘Dabinett’ ciders were 51% greater after short-term storage at 10 °C and 67% greater after long-term storage at 1 °C than the control, which was not subjected to a storage treatment. In 2016, total polyphenols in ‘Binet Rouge’ ciders were 67% greater after short-term storage at 10 °C and 94% greater after long-term storage at 1 °C than the control. Although results varied among cultivars and harvest years, storing apples for longer periods of time and at warmer temperatures may be a strategy to increase polyphenol, particularly flavanol, concentrations in hard cider.

scholarly journals Integrating Whole Blood Transcriptomic Collection Procedures Into the Current Anti-Doping Testing System, Including Long-Term Storage and Re-Testing of Anti-Doping Samples

2021 ◽  
Vol 8 ◽  
Author(s):  
Giscard Lima ◽  
Alexander Kolliari-Turner ◽  
Fernanda Rossell Malinsky ◽  
Fergus M. Guppy ◽  
Renan Paulo Martin ◽  
...  
Keyword(s):  
Whole Blood ◽  
Differentially Expressed ◽  
Rna Seq ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Short And Long Term ◽  
Rna Preservation ◽  
Term Storage

Introduction: Recombinant human erythropoietin (rHuEPO) administration studies involving transcriptomic approaches have demonstrated a gene expression signature that could aid blood doping detection. However, current anti-doping testing does not involve collecting whole blood into tubes with RNA preservative. This study investigated if whole blood in long-term storage and whole blood left over from standard hematological testing in short-term storage could be used for transcriptomic analysis despite lacking RNA preservation.Methods: Whole blood samples were collected from twelve and fourteen healthy nonathletic males, for long-term and short-term storage experiments. Long-term storage involved whole blood collected into Tempus™ tubes and K2EDTA tubes and subjected to long-term (i.e., ‒80°C) storage and RNA extracted. Short-term storage involved whole blood collected into K2EDTA tubes and stored at 4°C for 6‒48 h and then incubated at room temperature for 1 and 2 h prior to addition of RNA preservative. RNA quantity, purity, and integrity were analyzed in addition to RNA-Seq using the MGI DNBSEQ-G400 on RNA from both the short- and long-term storage studies. Genes presenting a fold change (FC) of >1.1 or < ‒1.1 with p ≤ 0.05 for each comparison were considered differentially expressed. Microarray analysis using the Affymetrix GeneChip® Human Transcriptome 2.0 Array was additionally conducted on RNA from the short-term study with a false discovery ratio (FDR) of ≤0.05 and an FC of >1.1 or < ‒1.1 applied to identify differentially expressed genes.Results: RNA quantity, purity, and integrity from whole blood subjected to short- and long-term storage were sufficient for gene expression analysis. Long-term storage: when comparing blood tubes with and without RNA preservation 4,058 transcripts (6% of coding and non-coding transcripts) were differentially expressed using microarray and 658 genes (3.4% of mapped genes) were differentially expressed using RNA-Seq. Short-term storage: mean RNA integrity and yield were not significantly different at any of the time points. RNA-Seq analysis revealed a very small number of differentially expressed genes (70 or 1.37% of mapped genes) when comparing samples stored between 6 and 48 h without RNA preservative. None of the genes previously identified in rHuEPO administration studies were differently expressed in either long- or short-term storage experiments.Conclusion: RNA quantity, purity, and integrity were not significantly compromised from short- or long-term storage in blood storage tubes lacking RNA stabilization, indicating that transcriptomic analysis could be conducted using anti-doping samples collected or biobanked without RNA preservation.

scholarly journals Age of Red Cells for Transfusion and Outcomes in Patients with ARDS

2022 ◽  
Vol 11 (1) ◽  
pp. 245
Author(s):  
Jan A. Graw ◽  
Victoria Bünger ◽  
Lorenz A. Materne ◽  
Alexander Krannich ◽  
Felix Balzer ◽  
...  
Keyword(s):  
Critically Ill ◽  
Critically Ill Patients ◽  
Hazard Ratio ◽  
Short Term ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Successful Weaning ◽  
The Mean ◽  
Term Storage

Packed red blood cells (PRBCs), stored for prolonged intervals, might contribute to adverse clinical outcomes in critically ill patients. In this study, short-term outcome after transfusion of PRBCs of two storage duration periods was analyzed in patients with Acute Respiratory Distress Syndrome (ARDS). Patients who received transfusions of PRBCs were identified from a cohort of 1044 ARDS patients. Patients were grouped according to the mean storage age of all transfused units. Patients transfused with PRBCs of a mean storage age ≤ 28 days were compared to patients transfused with PRBCs of a mean storage age > 28 days. The primary endpoint was 28-day mortality. Secondary endpoints included failure-free days composites. Two hundred and eighty-three patients were eligible for analysis. Patients in the short-term storage group had similar baseline characteristics and received a similar amount of PRBC units compared with patients in the long-term storage group (five units (IQR, 3–10) vs. four units (2–8), p = 0.14). The mean storage age in the short-term storage group was 20 (±5.4) days compared with 32 (±3.1) days in the long-term storage group (mean difference 12 days (95%-CI, 11–13)). There was no difference in 28-day mortality between the short-term storage group compared with the long-term storage group (hazard ratio, 1.36 (95%-CI, 0.84–2.21), p = 0.21). While there were no differences in ventilator-free, sedation-free, and vasopressor-free days composites, patients in the long-term storage group compared with patients in the short-term storage group had a 75% lower chance for successful weaning from renal replacement therapy (RRT) within 28 days after ARDS onset (subdistribution hazard ratio, 0.24 (95%-CI, 0.1–0.55), p < 0.001). Further analysis indicated that even a single PRBC unit stored for more than 28 days decreased the chance for successful weaning from RRT. Prolonged storage of PRBCs was not associated with a higher mortality in adults with ARDS. However, transfusion of long-term stored PRBCs was associated with prolonged dependence of RRT in critically ill patients with an ARDS.

When long-term learning depends on short-term storage

2017 ◽  
pp. 150-163
Author(s):  
A. D. Baddeley ◽  
C. Papagno ◽  
G. Vallar
Keyword(s):  
Short Term ◽  
Short Term Storage ◽  
Term Storage

Toward the Minimization of the Levelized Energy Costs of Microgrids Using Both Long-Term and Short-Term Storage Devices

Smart Grid ◽  
2017 ◽  
pp. 295-319
Author(s):  
Vincent François-Lavet ◽  
Quentin Gemine ◽  
Damien Ernst ◽  
Raphael Fonteneau
Keyword(s):  
Energy Costs ◽  
Short Term ◽  
Storage Devices ◽  
Short Term Storage ◽  
Term Storage

scholarly journals Profiles of 67Cu in blood, bile, urine and faeces from 67Cu-primed lambs: effect of 99Mo-labelled tetrathiomolybdate on the metabolism of 67Cu after long-term storage

1989 ◽  
Vol 61 (2) ◽  
pp. 373-385 ◽  
Author(s):  
S. R. Gooneratne ◽  
B. Laarveld ◽  
R. K. Chaplin ◽  
D. A. Christensen
Keyword(s):  
Dry Matter ◽  
Route Of Administration ◽  
Short Term ◽  
Storage Compartment ◽  
Long Term Storage ◽  
Short Term Storage ◽  
Term Storage

1. The effectiveness of tetrathiomolybdate (TTM) in the removal of 67Cu from the long-term storage compartment in liver was studied. Lambs receiving 5 mg Cu/kg dry matter (DM) or 35 mg Cu/kg DM were primed intravenously (iv) with 67Cu and challenged 10 d later with 99Mo-labelled TTM given either iv or intraduodenally (id). The profiles of 67Cu and 99Mo and of Cu and Mo with time were measured in blood, bile, urine and faeces.2. The level of dietary Cu affected the amplitude of profiles of 67Cu and Cu in blood, bile and urine after administration of 99Mo-labelled TTM. TTM administration increased liver Cu removal and this was most marked in sheep given TTM iv. The liver Cu removal from the long-term storage Cu compartment was low and was not affected by the route of administration of TTM. Endogenous Cu excretion was higher in lambs given TTM id.3. Excretion of 67Cu in bile through the transhepatocellular pathway after TTM administration appeared absent, while the transbiliary and hepatolysosomal pathways were operative. The potential reasons for this change are discussed.4. TTM predominantly enhances the removal of Cu from the short-term storage compartment, but effects on the long-term storage compartment may still be of significance.

scholarly journals PENGARUH TORSIO TESTIS TERHADAP GANGGUAN PROSES SPERMATOGENESIS PADA TESTIS TIKUS (Rattus norvegicus)

2017 ◽  
Vol 17 (2) ◽  
pp. 84-92
Author(s):  
Nora Adliyanti Basar ◽  
Dahril Dahril ◽  
Tita Menawati ◽  
Fajriah Fajriah
Keyword(s):  
Sertoli Cells ◽  
Rattus Norvegicus ◽  
Testicular Torsion ◽  
Round Spermatid ◽  
Term Effect ◽  
Short Term ◽  
Randomized Design ◽  
Long Term Effect ◽  
Completely Randomized Design

Abstrak. Torsio testis adalah terputarnya atau melilitnya korda spermatika, yang menyebabkan terputusnya aliran darah ke testis dan struktur jaringan di dalam skrotum. Penelitian yang bertujuan untuk mengidentifikasi efek variasi durasi waktu torsio testis terhadap proses spermatogenesis yang meliputi: jumlah sel Sertoli, spermatogonia, spermatosit primer, dan round spermatid testis Rattus norvegicus dilakukan dengan menggunakan rancangan acak lengkap (RAL) dan dibagi dalam 3 kelompok yaitu kontrol (KO) dan perlakuan (P1 dan P2). Kelompok perlakuan diinduksi torsio 360o pada testis kiri selama 4 dan 8 jam. Masing-masing kelompok terdiri dari 5 ekor tikus yang diamati langsung (efek cepat) dan 5 ekor tikus yang diamati setelah 30 hari dilakukan detorsi (efek lambat). Data dianalisa dengan metode ANOVA multifaktorial dan dilanjutkan dengan uji Tuckey HSD. Hasil penelitian menunjukkan bahwa rerata jumlah sel Sertoli pada KO: 142,00 ± 12,36; P1: 48,40 ± 8,00; P2: 35,70 ± 16,28, rerata jumlah spermatogonia KO: 975,30 ± 95,12; P1: 563,70 ± 170,44; P2: 321,10 ± 181,20, rerata jumlah spermatosit primer KO: 1307,50 ± 87,57; P1: 881,50 ± 253,65 ; P2: 505,80 ± 163,69, rerata round spermatid KO: 1237,20 ± 148,75; P1: 766,10 ± 277,68; P2: 473,00 ± 133,25. Berdasarkan hal tersebut, dapat disimpulkan bahwa torsio testis selama 4 dan 8 jam mengakibatkan perubahan jumlah sel Sertoli, spermatogonia, spermatosit primer, dan round spermatid (P0,05). Diharapkan, penanganan segera kasus torsio testis dapat menurunkan angka kerusakan testis.  (JKS 2017; 2: 83-93)Kata kunci : torsio testis, proses spermatogenesis, jumlah sel SertoliAbstract. Testicular torsion is the spermatic cord twisted, which causes the interruption of blood flow to the testicles and structures within the scrotum. The research was subjected to identify the effect of various duration of testicular torsion to spermatogenesis process include on the amount of Sertoli cells, spermatogonia, primary spermatocytes, and spermatids round of Rattus norvegicus testes. This experimental research using completely randomized design (CRD) and contains of 3 groups: control (KO) and experimental groups (P1 and P2). The experimental groups which contains 20 rats underwent 360o unilateral left testicular torsion for 4 hours (P1) and 8 hours (P2). Five rats for each groups were examined after testicular torsion induced (short term effect) besides five other remained were examined after 30 days testicular tortion repaired (long term effect). Data were analyzed using ANOVA multifactorial followed by Tuckey’s HSD test. The result showed significant differences (P0,05). Between KO: 142.00 ± 12.36; P1: 48.40 ± 8.00; and P2: 35.70 ± 16.28 in the amount of Sertoli cells, between KO: 975.30 ± 95, 12: P1: 563.70 ± 170.44; P2: 321.10 ± 181.20 in the amount of spermatogonia, between KO: 1307.50 ± 87.57; P1: 881.50 ± 253.65; P2: 505, 80 ± 163.69 in the amount of primary spermatocytes, between KO: 1237.20 ± 148.75; P1: 766.10 ± 277.68; P2: 473.00 ± 133,25 in the amount of spermatids round. Therefore, testicular torsion for 4 and 8 hours resulted in changes in the amount of Sertoli cells, spermatogonia, primary spermatocytes, and spermatids round (P0,05). Expected, the immediate treatment of cases of testicular torsion may reduce the number of testicular damage. (JKS 2017; 2: 83-93)Keywords: testicular torsion, spermatogenesis process, the amount of Sertoli cell

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