Antibacterial Activities of Essential Oils from Three Medicinal Plants in Combination with EDTA against Methicillin-resistant Staphylococcus aureus

2016 ◽  
Vol 17 (4) ◽  
pp. 1-10 ◽  
Author(s):  
Juan Armas ◽  
Julio Quiroz ◽  
Robert Roman ◽  
Jose Sanchez ◽  
Miriam Pacheco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plants ◽  
Essential Oils ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Methicillin Resistant

scholarly journals Antibacterial activity of endophytic fungi in Pometia pinnata against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Ratna Setyaningsih ◽  
Ari Susilowati ◽  
Dina Prasetyani
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Endophytic Fungi ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Its Region ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Bacterial Cells ◽  
Methicillin Resistant

Abstract. Endophytic fungi associated with medicinal plants are able to produce bioactive compounds which potentially contain medicinal ingredients. Matoa (Pometia pinnata J. R. Forst & G. Forst) is one of Indonesian medicinal plants. This research aims to test antibacterial activities of ethyl acetate extract of matoa’s endophytic fungi to Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) and also identify isolates having high antibacterial activity. Initial test on antibacterial activity was conducted by using paper disc diffusion, while advanced test was conducted by determining minimum inhibitory concentration (MIC). The fungi were identified in morphological and molecular manner with amplification and sequencing of ITS region. Bacterial cells exposed to the extract were observed by using scanning electron microscope (SEM). Isolate AM72 was found out to have the highest antibacterial activity compared to other 12 isolates tested, with inhibition zone diameter of 31.57 mm to S. aureus and 22.87 mm to MRSA. Isolate AM72 was identified as Lasiodiplodia theobromae. Isolate BMB identified as Aspergillus oryzae produced inhibition zone with 21.96 mm diameter to S. aureus and 21.50 mm diameter to MRSA. Advanced test to isolate AM72 gave the same MIC results to S. aureus and MRSA of 1.56%. Antibacterial compound produced by isolate AM72 were found out to be able to damage bacterial cell walls. Keywords: Antibacterial, Endohpytic Fungi, Matoa, S. aureus, MRSA  

Essential oils from unexplored aromatic plants quench biofilm formation and virulence of Methicillin resistant Staphylococcus aureus

2018 ◽  
Vol 122 ◽  
pp. 162-173 ◽  
Author(s):  
Durairajan Rubini ◽  
Sanaulla Farisa Banu ◽  
Prakash Nisha ◽  
Ramar Murugan ◽  
Subbiah Thamotharan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Aromatic Plants ◽  
Methicillin Resistant

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oils from Ferula L. Species against Methicillin-Resistant Staphylococcus aureus

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1679 ◽  
Author(s):  
Gulzhakhan Utegenova ◽  
Kyler Pallister ◽  
Svetlana Kushnarenko ◽  
Gulmira Özek ◽  
Temel Özek ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant

Antibacterial Activity of Thai Medicinal Plants against Methicillin-resistant Staphylococcus aureus

Fitoterapia ◽  
2009 ◽  
Vol 80 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Mullika Traidej Chomnawang ◽  
Suvimol Surassmo ◽  
Karn Wongsariya ◽  
Nuntavan Bunyapraphatsara
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistant ◽  
Thai Medicinal Plants

scholarly journals Antibacterial of Cinnamon Bark (Cinnamomum burmannii) Essential Oil Against Methicillin-Resistant Staphylococcus aureus

2021 ◽  
Vol 4 (1) ◽  
pp. 56
Author(s):  
Shinta Levea Ni'matul Fadlilah ◽  
Mustofa Helmi Effendi ◽  
Wiwiek Tyasningsih ◽  
Lucia Tri Suwanti ◽  
Jola Rahmahani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Methicillin Resistant ◽  
Cinnamon Bark ◽  
Cinnamomum Burmannii

This study aimed to determine the in vitro antibacterial activities of essential oil from cinnamon bark (Cinnamomum burmannii) on Methicillin-resistant Staphylococcus aureus (MRSA) isolated from raw milk. Essential oil from cinnamon bark obtained from the steam distillation method and essential oil was made in a series dilution with a concentration of 1%, 2%, 4%, and 8%. The antibacterial activities were tested using the disk diffusion method. Results showed from five isolates of MRSA, one isolate was sensitive of essential oil with a concentration 2% and all of the isolate was sensitive with a concentration 4% and 8%. The antibacterial activity showed by inhibition zones on  MRSA. The results suggest that the activity of the essential oils of cinnamon bark has an antibacterial effect on MRSA and it is developed as phytopharmaca.

scholarly journals Biofilm Inhibition and Eradication Properties of Medicinal Plant Essential Oils against Methicillin-Resistant Staphylococcus aureus Clinical Isolates

2020 ◽  
Vol 13 (11) ◽  
pp. 369
Author(s):  
Fethi Ben Abdallah ◽  
Rihab Lagha ◽  
Ahmed Gaber
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Isolates ◽  
Mass Spectrometry Analysis ◽  
Biofilm Inhibition ◽  
Methicillin Resistant ◽  
Plant Essential Oils

Methicillin-resistant Staphylococcus aureus is a major human pathogen that poses a high risk to patients due to the development of biofilm. Biofilms, are complex biological systems difficult to treat by conventional antibiotic therapy, which contributes to >80% of humans infections. In this report, we examined the antibacterial activity of Origanum majorana, Rosmarinus officinalis, and Thymus zygis medicinal plant essential oils against MRSA clinical isolates using disc diffusion and MIC methods. Moreover, biofilm inhibition and eradication activities of oils were evaluated by crystal violet. Gas chromatography–mass spectrometry analysis revealed variations between oils in terms of component numbers in addition to their percentages. Antibacterial activity testing showed a strong effect of these oils against MRSA isolates, and T. zygis had the highest activity succeeded by O. majorana and R. officinalis. Investigated oils demonstrated high biofilm inhibition and eradication actions, with the percentage of inhibition ranging from 10.20 to 95.91%, and the percentage of eradication ranging from 12.65 to 98.01%. O. majorana oil had the highest biofilm inhibition and eradication activities. Accordingly, oils revealed powerful antibacterial and antibiofilm activities against MRSA isolates and could be a good alternative for antibiotics substitution.

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