Determination of Lactic Acid Content during Embryonic Development of the Silkworm under Imposed Photoperiodic Regimes

2021 ◽  
pp. 76-80
Author(s):  
Jyoti Prasad Baruah ◽  
T. S. Jagadeesh Kumar ◽  
Chinmoyee Kalita ◽  
K. M. Arun Kumar ◽  
G. Impana
2006 ◽  
Vol 29 (13) ◽  
pp. 1905-1916 ◽  
Author(s):  
John McHugh ◽  
Suzanne N. Dumont ◽  
Julie Paradis ◽  
Anh Loan Nguyen ◽  
Suzanne Levesque ◽  
...  

1980 ◽  
Vol 43 (7) ◽  
pp. 558-560 ◽  
Author(s):  
B. MORENO ◽  
A. CALLES ◽  
V. DIEZ

Occurrence of non-specific inhibition zones with concentrated chicken muscle extracts depends on the pH of the extract being assayed. Buffering of the extraction residues with a phosphate buffer of pH 7.0 greatly reduced but did not completely prevent appearance of non-specific inhibition zones. Haloes appearing with buffered muscle extracts were more frequent and intense as post mortem time increased. Inhibition was not dependent on pH per senor on lactic acid content, at least within the physiological range; neither was it influenced in this range by the combined pH-lactic acid content. The residue of the extraction solvents (oxalate-EDTA-acetone) was not responsible for this inhibition at physiological pH values. From the results obtained, it seems reasonable to associate the non-specific inhibition zones with substances produced in the muscle post-mortem.


2019 ◽  
Vol 15 (4) ◽  
pp. 511-521 ◽  
Author(s):  
Merve Eylul Kiymaci ◽  
Mehmet Gumustas ◽  
Nurten Altanlar ◽  
Ahmet Akin ◽  
Aysegul Zenciroglu ◽  
...  

Background:Probiotics are living microorganisms that have a healthy influence on a host.Objective:The aim of this study was to isolate a probiotic Pediococcus acidilactici strain from newborn faeces and develop and optimize a selective high-performance liquid chromatography method for the determination and validation of its lactic acid content and also evaluate some probiotic characteristics.Methods:Isolated strains were identified by the API 50 CH system and 16S rDNA gene sequence analysis and tested for antibiotic susceptibility, bile salt tolerance, low pH resistance, proteolytic, haemolytic activity, as well as the production of bacteriocin, hydrogen peroxide, and lactic acid. Antimicrobial activity of selected strain against standard test microorganisms was determined by the spot lawn method and the quantitation of lactic acid was carried out by high-performance liquid chromatography on a Rezex ROA organic acid (300x7.8 mm) analytical column.Results:P. acidilactici M7 strain was evaluated as a potential probiotic due to its ability to survive at low pH values or in the presence of pepsin, pancreatin, and bile salts. The lactic acid amount of strain was found in the range between 5.59-5.94 mg mL-1 by HPLC. M7 strain was also found to be resistant to vancomycin, had no bacteriocin, and hydrogen peroxide production and was able to inhibit the growth of P. aeruginosa and E. faecalis by its lactic acid content.Conclusion:This study explains a simple, selective, and fully validated procedure for the determination of lactic acid from probiotic bacteria.


2011 ◽  
Vol 24 (1) ◽  
pp. 158-164 ◽  
Author(s):  
Zsuzsanna Bori ◽  
Gábor Csiffáry ◽  
Diána Virág ◽  
Marianna Tóth-Markus ◽  
Attila Kiss ◽  
...  

1935 ◽  
Vol 110 (3) ◽  
pp. 637-642
Author(s):  
Bennett F. Avery ◽  
Stanley E. Kerr ◽  
Musa Ghantus

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Yoshimura K ◽  
Inoue Y ◽  
Koizumi A ◽  
Suzuki M ◽  
Itakura S ◽  
...  

Purpose: The aims of this study were to prepare a 0.1% Miconazole (MCZ) eye-drop solution and to evaluate the stability and physical properties of the preparation.


Rangifer ◽  
1982 ◽  
Vol 2 (1) ◽  
pp. 31
Author(s):  
Ulla-Britt Bøe ◽  
Nicolai Gundersen ◽  
Harald Sletten ◽  
Endre Jacobsen

<p>Feed intake, general condition, rumenal pH and lactic acid content was followed during feeding of starved reindeer on a coventional diet (RF-71). The result was a dramatic decrease of rumen pH (pH=5.1 in experiment no. Ill) a few hours after feeding of the coventional diet, but this could be counteracted significantly by addition of buffer (pH=6.4 in experiment no. Ill) of hydrogenated marine oil (pH=6.1 in experiment no. Ill) to the ration. The highest content of the lactic acid in the rumen was 40 mM in animals fed RF-71, whereas addition of buffer or hydrogenated marine oil completely supressed accumulation of this acid.</p><p>Foropptak, pH og melkesyre i vomma hos rein under overgangsforing med kraftfor tilsatt buffer og fett.</p><p>Abstract in Norwegian&nbsp;/ Sammendrag: Forinntak, almentilstand, pH og melkesyreinnhold i vom ble registrert under foring av sultede rein med konvensjonelt kraftfor (reinfor 71 = RF-71). Det ble funnet et sterkt pH-fall i vomma (pH=5.1, fors&oslash;k III) f&aring; timer etter at dyrene startet &aring; spise RF-71. Denne effekten ble redusert ved tilsetting av buffer (pH=6.4, fors&oslash;k III) eller herdet marint fett (pH=6.1, fors&oslash;k III) til dietten. De dyr som ble foret med RF-71 hadde et melkesyreinnhold i vomma p&aring; opp til 40 mM. Tilsetting av buffer eller herdet marint fett til dietten forhindret akkumulering av melkesyre.</p><p>Poron rehunk&auml;ytt&ouml;, p&ouml;tsin pH ja maitohapposis&auml;lt&ouml; siirtym&auml;vaiheen ruokinnassa k&auml;ytett&auml;ess&auml; v&auml;kirehua, johon on lis&auml;tty puskuria ja rasvaa.</p><p>Abstract in Finnish / Yhteenveto: Rehunk&auml;ytto&auml;, yleistilaa, p&ouml;tsin pH:ta ja maitohapposis&auml;lt&ouml;&auml; tutkittiin ruokittaessa n&auml;lkiintyneit&auml; poroja tavanomaisella v&auml;kirehulla (poron 71 = RF-71). Joitakin tuntejaruokinnan aloittamisen j&auml;lkeen havaittiin voimakas p&ouml;tsin pH:n lasku (pH 5.1, koe III). T&auml;m&auml; vaikutus v&auml;heni kun poron nauttiman ravinnon joukkoon lis&auml;ttiin puskuria (pH 6.4, koe III) tai merikaloista valmistettua kovetettua rasvaa (pH 6.1, koe III). RF-71 rehua nauttineiden el&auml;inten p&ouml;tsin maitohapposis&auml;lt&ouml; oli 40 nM. Puskurin tai kovetetun merellisen el&auml;inper&auml;isen rasvan lis&auml;&auml;minen ravintoon ehk&auml;isi maitohapon kasaantumista.</p>


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