Kaurenoic Acid Isolated from the Root Bark of Annona senegalensis Induces Cytotoxic and Antiproliferative Effects against PANC-1 and HeLa cells

The antiproliferative activities of 12-oxoheteronemin and heteronemin were evaluated in six cancer cell lines and IC50 values ranging from 0.66 to 1.35 μM were obtained. In four of the cell lines, 12-oxoheteronemin and heteronemin were equipotent; however, in two estrogenic receptor-positive cell lines, heteronemin showed a stronger potency. Both compounds had no overt effects on cell cycle distribution in HeLa cells, but did rapidly initiate apoptosis as evidenced by increased sub-G1 populations of cells and caspase-dependent PARP cleavage.

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Cytotoxic Prenylated Polyphenolic Compounds from Maackia amurensis Root Bark

Natural Product Communications ◽

10.1177/1934578x1701200709 ◽

2017 ◽

Vol 12 (7) ◽

pp. 1934578X1701200

Author(s):

Marina V. Veselova ◽

Sergey A. Fedoreyev ◽

Darya V. Tarbeeva ◽

Nadezda I. Kulesh ◽

Anatoliy I. Kalinovskiy ◽

...

Keyword(s):

Cytotoxic Activity ◽

Cell Lines ◽

Hela Cells ◽

Human Cancer ◽

Polyphenolic Compounds ◽

Root Bark ◽

Human Cancer Cell ◽

Human Cancer Cell Lines ◽

Maackia Amurensis ◽

New Compounds

Two new prenylated flavanones, isomaackiaflavanone A (1), isomaackiaflavanone B (2) and a new prenylated stilbene maackiastilbene (3), along with five known flavanones were isolated from the root bark of Maackia amurensis using repeated column chromatography on silica gel, Sephadex LH-20 and octadecyl silica (ODS-A) sorbents. The structures of the new compounds were elucidated by HPLC–PDA–MS, HR-ESI-MS, 1H, 13C, 1H–1H COSY, HSQC, ROESY and HMBC NMR analyses. The cytotoxicity of compounds 1–2, 4–8 against two human cancer cell lines HeLa and SK-MEL-5 was tested using the MTS method. Compounds 4, 6 and 8 showed the strongest cytotoxic activity among the compounds tested with IC50 values of 6.5, 8.8 and 7.7 μM, against SK-MEL-5 cells and 8.2, 18.8 and 12 μM against HeLa cells, respectively.

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Review of "Anticonvulsant effect of kaurenoic acid isolated from the root bark of Annona senegalensis"

Publons reviews and discussion ◽

10.14322/publons.r236505 ◽

2016 ◽

Author(s):

Krishnadas Nandakumar

Keyword(s):

Anticonvulsant Effect ◽

Root Bark ◽

Kaurenoic Acid

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Antiproliferative effects of some medicinal plants on HeLa cells

Archives of Biological Sciences ◽

10.2298/abs1301065m ◽

2013 ◽

Vol 65 (1) ◽

pp. 65-70 ◽

Cited By ~ 2

Author(s):

Desanka Cenic-Milosevic ◽

Z. Tambur ◽

D. Bokonjic ◽

S. Ivancajic ◽

Tatjana Stanojkovic ◽

...

Keyword(s):

Medicinal Plants ◽

Hela Cell ◽

Cytotoxic Activity ◽

Cell Lines ◽

Hela Cells ◽

Melissa Officinalis ◽

Echinacea Angustifolia ◽

Antiproliferative Effects ◽

Hela Cell Lines ◽

Ic50 Value

Medicinal plants maintain the health and vitality of individuals, and also have potential curative effect on various diseases, including cancer. In this study were investigated the antiproliferative effects of water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Echinacea angustifolia, Salvia officinalis and Melissa officinalis) on cell lines derived from human cervix adenocarcinoma (HeLa cells). The best cytotoxic activity (IC50 = 43.52 ?g/ml) on HeLa cell lines was exhibited by Echinacea angustifolia. The extract of Salvia officinalis also showed a good cytotoxic activity against HeLa cell lines; the IC50 value was 70.41 ?g/ml. Melissa officinalis manifested a slightly weaker cytotoxic activity and an IC50 value of 122.22 ?g/ml.

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Antiproliferative Effects of AAV-Delivered CRISPR/Cas9-Based Degradation of the HPV18-E6 Gene in HeLa Cells

10.21203/rs.3.rs-602201/v1 ◽

2021 ◽

Author(s):

Zahra Noroozi ◽

Mehdi Shamsara ◽

Elahe Valipour ◽

Sahar Esfandyari ◽

Alireza Ehghaghi ◽

...

Keyword(s):

Hela Cells ◽

P53 Protein ◽

Annexin V ◽

Antiproliferative Effects ◽

Cervical Cancers ◽

Hpv E6 ◽

E6 Gene ◽

Therapeutic Benefits ◽

Hpv18 E6 ◽

Infected Cells

Abstract Human papillomavirus infections are associated with most cervical cancers, which are the fourth most common cancer in women. HPV-E6 protein binds to protein p53 and inhibits its function, leading to the switching of normal cells toward cancer cells. Here, we disrupted the HPV-E6 gene and investigated its effects on the proliferation and apoptosis of HeLa cells. The HPV18-E6 gene was targeted with two designed sgRNAs cloned into an AAV-CRISPR-based plasmid. The AAV-E6-CRISPR/Cas9 virions were prepared and titrated in HEK293t cells. The cleavage created in the HPV-E6 gene was detected using the T7E1 assay. Cell cycle profiling, MTT assay, and annexin V/PI staining were performed. Also, the p53 protein level was measured by Western blotting. Our data showed that disruption of the HPV-E6 gene led to increased cell apoptosis and decreased cell proliferation. A significant accumulation of infected cells in sub-G1 phase was observed in the cell profiling assay. Also, HPV-E6 gene disruption resulted in a significant increase in the level of P53 protein. Our findings indicated that AAV-mediated delivery of CRISPR/Cas9 can effectively target the HPV-E6 gene in HeLa cells, and its antiproliferative effects may provide therapeutic benefits of local administration of this gene-editing system for HPV-related cervical cancers.

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