Antibacterial and anticancer activity of kaurenoic acid from root bark extract of Annona senegalensis

3,5-disubstituted-1,2,4-tiadiazoles with substituted-styril and heterocycle-vinyl were obtained by extending the method which implies the use of 3,5-disubstituted-1,2,4-ditiazolium salts as precursors [1-4]. A comparative study of the reaction process in the case of perchlorates, diacide phosphates, tribromides and 3,5-distyrile-dithiazolium triiodides, taken as etalon, was perfomed. Good yields were obtained when using perchlorates, phosphates and triiodides. The reaction was made by heating the reaction mixture and by ultrasound exposure. The structure of the compounds was confirmed by chemical and physical analysis and the data obtained were identical to those of 3,5-disubstituted-1,2,4-tiadiazoles obtained by another methods [5,6]. The biological (antibacterial and anticancer) activity of the synthesized compounds was tested and the results indicated a medium activity.

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Synthesis, Spectral Characterization, Antibacterial and Anticancer Activity of some Titanium Complexes

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520618666171219122431 ◽

2018 ◽

Vol 18 (5) ◽

pp. 739-746 ◽

Cited By ~ 2

Author(s):

Raj Kaushal ◽

Nitesh Kumar ◽

Archana Thakur ◽

Kiran Nehra ◽

Pamita Awasthi ◽

...

Keyword(s):

Cell Cycle ◽

Cell Death ◽

Anticancer Activity ◽

Cell Lines ◽

Spectral Characterization ◽

Mechanistic Study ◽

Titanium Complexes ◽

G0 Phase ◽

Antibacterial And Anticancer Activity ◽

Dose Dependent

Abstract: Background: After the discovery of cisplatin, first non platinum anticancer drugs having excellent efficacy were budotitane and TiCl2(cp)2 but action mechanism is not clear. Therefore, we hereby reporting synthesis and biological activities novel titanium complexes to explore their mode of action. Objectives: Synthesis, spectral characterization, antibacterial and anticancer activity of some titanium complexes. Antibacterial studies on various bacterial strains and anticancer studies on HeLa, C6, CHO cancerous cell lines have been performed. Further, the cell death mechanistic study was done on CHO cell lines. Method: Titanium complexes with and without labile groups have been synthesized by reacting of TiCl4 with nitrogen containing ligands viz. 1,2-diaminocyclohexane, 1,10-Phenanthroline, adamantylamine, 2,2'-bipyridine, 4,4'-dimethyl-2,2'-bipyridine in predetermined molar ratios. Antibacterial and anticancer studies were performed by agar well diffusion method and MTT assay respectively. Cell cycle analysis is done by using flow cytometry. Results: Complex 2 i.e TiCl2(Phen)2 showed better activity than other complexes as an antibacterial as well as anticancer agent. Phase contrast imaging indicates that observed morphological changes of cells was dose dependent. Cell death mechanistic study have shown the increase in sub G0 phase population as well as formation of blebbing and fragmentation of chromatin material which is an indicative measure of apoptosis. Conclusion: Complex 2 proved to be more effective bactericide and cytotoxic agent. Cell cycle analysis showed cell arrest in G0 phase. Apoptosis percentage was found to increase in a dose dependent manner. So, prepared titanium complexes can be put to use as an important chemotherapeutic agents.

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A novel herbometallic nanodrug has the potential for antibacterial and anticancer activity through oxidative damage

Nanomedicine ◽

10.2217/nnm-2018-0187 ◽

2019 ◽

Vol 14 (9) ◽

pp. 1173-1189 ◽

Cited By ~ 4

Author(s):

Bhuban Ruidas ◽

Sutapa Som Chaudhury ◽

Kunal Pal ◽

Prashanta Kumar Sarkar ◽

Chitrangada Das Mukhopadhyay

Keyword(s):

Oxidative Damage ◽

Anticancer Activity ◽

Antibacterial And Anticancer Activity

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Vascular Protection by Ethanol Extract of Morus alba Root Bark: Endothelium-Dependent Relaxation of Rat Aorta and Decrease of Smooth Muscle Cell Migration and Proliferation

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2018/7905763 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 4

Author(s):

Nisha Panth ◽

Keshav Raj Paudel ◽

Dal-Seong Gong ◽

Min-Ho Oak

Keyword(s):

Nitric Oxide ◽

Smooth Muscle ◽

Morus Alba ◽

Guanosine Monophosphate ◽

Current Evidence ◽

Bark Extract ◽

Root Bark ◽

Natural Medicine ◽

And Migration ◽

Proliferation And Migration

Morus alba (white mulberry) is native to the northern part of Korea and popularly used as a traditional medicine due to its numerous health benefits against human’s disease. However, the possibility that M. alba may also affect the cardiovascular system remains unexplored. This study sought to investigate the vascular protective effects of the root bark extract of M. alba (MAE). Vascular reactivity was performed in organ baths using isolated rat thoracic aorta, while platelet derived growth factor (PDGF) induced proliferation and migration of vascular smooth muscle cells (VSMCs) were studied by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) and wound healing assay, respectively. MAE evoked a concentration dependent vasorelaxation following endothelium-dependent pathway. However, vessel relaxations in response to MAE were markedly reduced after endothelium removal; treatment of endothelial nitric oxide synthase inhibitor, guanylyl cyclase inhibitor, and nonspecific potassium channel inhibitor, however, was not altered by cyclooxygenase inhibitor. Furthermore, MAE also significantly blunted contractile response to vasoconstrictor agent, phenylephrine. Taken together, the current evidence revealed that MAE is a potent endothelium-dependent vasodilator and this effect was involved in, at least in part, nitric oxide cyclic-guanosine monophosphate (NO-cGMP) pathway in combination with potassium (K+) channel activation. Moreover, MAE inhibited proliferation and migration of VSMCs induced by PDGF. Therefore, MAE could be a promising candidate of natural medicine for preventing and controlling cardiovascular diseases linked with endothelial dysfunction.

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Antidepressant activity of methanol root bark extract of Securinega virosa (Ex Willd.) Baill in albino mice

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v10i2.45 ◽

2018 ◽

Vol 10 (2) ◽

pp. 277

Author(s):

A Shehu ◽

M.G. Magaji ◽

B Sanni ◽

S.N. Abdu-Aguye

Keyword(s):

Antidepressant Activity ◽

Bark Extract ◽

Root Bark ◽

Albino Mice ◽

Securinega Virosa

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Bacteriostatic and Bactericidal effects of ethyl acetate root bark extract of Terminalia avicennioides on Methicillin-resistant Staphylococcus aureus

African Journal of Biochemistry Research ◽

10.5897/ajbr2017.0981 ◽

2018 ◽

Vol 12 (5) ◽

pp. 45-54

Author(s):

Ekene Ugochukwu Adim ◽

Emeka John Dingwoke ◽

Fatima Amin Adamude ◽

Chidi Edenta ◽

Ndubuisi Nwobodo Nwobodo ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Ethyl Acetate ◽

Methicillin Resistant Staphylococcus Aureus ◽

Bark Extract ◽

Root Bark ◽

Methicillin Resistant ◽

Bactericidal Effects

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Effect of Morus alba root bark extract on gene-level expression of inflammatory markers in rats subjected to ethanol and cerulein induced pancreatitis– influence of heat shock protein 70

Journal of Complementary and Integrative Medicine ◽

10.1515/jcim-2017-0149 ◽

2018 ◽

Vol 16 (2) ◽

Author(s):

Kavitha Yuvaraj ◽

Arumugam Geetha

Keyword(s):

Oxidative Stress ◽

Heat Shock ◽

Morus Alba ◽

Bark Extract ◽

Root Extract ◽

Root Bark ◽

Nf Kappa B ◽

Serum Lipase ◽

Hsp70 Mrna ◽

Caspase 1

Abstract Background Chronic pancreatitis (CP) is a persistent inflammation of the pancreas clinically presented with severe abdominal pain, progressive fibrosis, and loss of exocrine and endocrine functions. Inflammasomes, cytosolic multiprotein complexes which regulate the formation of proinflammatory cytokines, are influenced by various factors including heat shock proteins (HSPs). Morus alba L., or white mulberry root bark is a valued traditional Asian medicine with a diverse array of phytochemicals. The aim of this investigation was to define the modulatory action of methanolic extract of Morus alba root bark (MEMARB) on NLRP3 inflammasome, and HSPs in pancreas subjected to inflammatory insult. Methods Pancreatitis was induced in male albino Wistar rats by ethanol (0–36%) and cerulein (20 µg/kg b.wt., i.p.) for 5 weeks with or without MEMARB administration. Serum lipase/amylase (L/A) ratio, oxidative stress index (OSI) and reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio in the pancreas were evaluated. Levels of serum HSP70 was quantified by ELISA. NF-kappa B, NLRP3-ASC, caspase-1, IL-1β, IL-18, and HSP70 gene expression was quantified by quantitative real-time polymerase chain reaction (qPCR). Results L/A ratio and oxidative stress determined in terms of OSI and GSH/GSSG ratio were elevated in pancreatitis-induced rats. The levels were restored in MEMARB co-administered animals. Serum level of HSP70 was increased in pancreatitis-induced animals and dropped significantly in MEMARB co-administrated rats. Pancreatitis-induced group showed increased expression of NF-kappa B, IL-1β, IL-18, caspase-1, NLRP3-ASC and HSP70 mRNA than in MEMARB treated group. Conclusions It can be concluded that the M. alba root extract modulates the expression of HSP70 and NLRP3-ASC which might be attributed to its pancreato-protective effect.

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