scholarly journals Mono-anionic phosphopeptides produced by unexpected histidine alkylation exhibit high plk1 polo-box domain-binding affinities and enhanced antiproliferative effects in hela cells

Biopolymers ◽  
2014 ◽  
Vol 102 (6) ◽  
pp. 444-455 ◽  
Author(s):  
Wen-Jian Qian ◽  
Jung-Eun Park ◽  
Dan Lim ◽  
Christopher C. Lai ◽  
James A. Kelley ◽  
...  
Keyword(s):  
Hela Cells ◽  
Binding Affinities ◽  
Antiproliferative Effects

scholarly journals Antiproliferative Effects of 12-Oxoheteronemin vs Heteronemin

2014 ◽  
Vol 9 (3) ◽  
pp. 1934578X1400900
Author(s):  
Siriporn Kittiwisut ◽  
Cristina C. Rohena ◽  
Supreeya Yuenyongsawad ◽  
Susan L. Mooberry ◽  
Anuchit Plubrukarn
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Cancer Cell ◽  
Hela Cells ◽  
Cancer Cell Lines ◽  
Parp Cleavage ◽  
Cell Cycle Distribution ◽  
Antiproliferative Effects ◽  
Antiproliferative Activities

The antiproliferative activities of 12-oxoheteronemin and heteronemin were evaluated in six cancer cell lines and IC50 values ranging from 0.66 to 1.35 μM were obtained. In four of the cell lines, 12-oxoheteronemin and heteronemin were equipotent; however, in two estrogenic receptor-positive cell lines, heteronemin showed a stronger potency. Both compounds had no overt effects on cell cycle distribution in HeLa cells, but did rapidly initiate apoptosis as evidenced by increased sub-G1 populations of cells and caspase-dependent PARP cleavage.

scholarly journals Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Carla Oliveira ◽  
Ana Nicolau ◽  
José A. Teixeira ◽  
Lucília Domingues
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Hela Cells ◽  
Carbohydrate Binding ◽  
Binding Affinities ◽  
Proliferation And Apoptosis ◽  
Cervical Cancer Cells ◽  
Different Sources ◽  
Binding Lectin ◽  
Galactose Binding Lectin

Frutalin is theα-D-galactose-binding lectin isolated from breadfruit seeds. Frutalin was obtained from two different sources: native frutalin was purified from its natural origin, and recombinant frutalin was produced and purified fromPichia pastoris. This work aimed to study and compare the effect of native and recombinant frutalin on HeLa cervical cancer cells proliferation and apoptosis. Furthermore, the interaction between frutalin and the HeLa cells was investigated by confocal microscopy. Despite having different carbohydrate-binding affinities, native and recombinant frutalin showed an identical magnitude of cytotoxicity on HeLa cells growth (IC50~100 μg/mL) and equally induced cell apoptosis. The interaction studies showed that both lectins were rapidly internalised and targeted to HeLa cell's nucleus. Altogether, these results indicate that frutalin action is not dependent on its sugar-binding properties. This study provides important information about the bioactivity of frutalin and contributes to the understanding of the plant lectins cytotoxic activity.

scholarly journals Antiproliferative effects of some medicinal plants on HeLa cells

2013 ◽  
Vol 65 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Z. Tambur ◽  
D. Bokonjic ◽  
S. Ivancajic ◽  
Tatjana Stanojkovic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Hela Cells ◽  
Melissa Officinalis ◽  
Echinacea Angustifolia ◽  
Antiproliferative Effects ◽  
Hela Cell Lines ◽  
Ic50 Value

Medicinal plants maintain the health and vitality of individuals, and also have potential curative effect on various diseases, including cancer. In this study were investigated the antiproliferative effects of water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Echinacea angustifolia, Salvia officinalis and Melissa officinalis) on cell lines derived from human cervix adenocarcinoma (HeLa cells). The best cytotoxic activity (IC50 = 43.52 ?g/ml) on HeLa cell lines was exhibited by Echinacea angustifolia. The extract of Salvia officinalis also showed a good cytotoxic activity against HeLa cell lines; the IC50 value was 70.41 ?g/ml. Melissa officinalis manifested a slightly weaker cytotoxic activity and an IC50 value of 122.22 ?g/ml.

Antiproliferative Effects of AAV-Delivered CRISPR/Cas9-Based Degradation of the HPV18-E6 Gene in HeLa Cells

2021 ◽  
Author(s):  
Zahra Noroozi ◽  
Mehdi Shamsara ◽  
Elahe Valipour ◽  
Sahar Esfandyari ◽  
Alireza Ehghaghi ◽  
...  
Keyword(s):  
Hela Cells ◽  
P53 Protein ◽  
Annexin V ◽  
Antiproliferative Effects ◽  
Cervical Cancers ◽  
Hpv E6 ◽  
E6 Gene ◽  
Therapeutic Benefits ◽  
Hpv18 E6 ◽  
Infected Cells

Abstract Human papillomavirus infections are associated with most cervical cancers, which are the fourth most common cancer in women. HPV-E6 protein binds to protein p53 and inhibits its function, leading to the switching of normal cells toward cancer cells. Here, we disrupted the HPV-E6 gene and investigated its effects on the proliferation and apoptosis of HeLa cells. The HPV18-E6 gene was targeted with two designed sgRNAs cloned into an AAV-CRISPR-based plasmid. The AAV-E6-CRISPR/Cas9 virions were prepared and titrated in HEK293t cells. The cleavage created in the HPV-E6 gene was detected using the T7E1 assay. Cell cycle profiling, MTT assay, and annexin V/PI staining were performed. Also, the p53 protein level was measured by Western blotting. Our data showed that disruption of the HPV-E6 gene led to increased cell apoptosis and decreased cell proliferation. A significant accumulation of infected cells in sub-G1 phase was observed in the cell profiling assay. Also, HPV-E6 gene disruption resulted in a significant increase in the level of P53 protein. Our findings indicated that AAV-mediated delivery of CRISPR/Cas9 can effectively target the HPV-E6 gene in HeLa cells, and its antiproliferative effects may provide therapeutic benefits of local administration of this gene-editing system for HPV-related cervical cancers.

scholarly journals Antiproliferative effects of Camellia sinensis, Frangula alnus and Rosmarinus officinalis

2013 ◽  
Vol 65 (3) ◽  
pp. 885-891 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Z. Tambur ◽  
S. Ivancajic ◽  
D. Bokonjic ◽  
Anika Cukovic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Camellia Sinensis ◽  
Hela Cells ◽  
Cytotoxic Effect ◽  
Geographic Origin ◽  
Rosmarinus Officinalis ◽  
Cancer Growth ◽  
Antiproliferative Effects ◽  
Frangula Alnus ◽  
Human Cervix

The use of medicinal plants is becoming increasingly appreciated in suppressing cancer growth and cancer prevention. In this study the antiproliferative effects of the water extracts of previously obtained ethanolic dry extracts of three different medicinal plants (Camellia sinensis, Frangula alnus from two different places and Rosmarinus officinalis) using cell lines derived from human cervix adenocarcinoma (Hela cells) were investigated. The extract of Camellia sinensis exhibited significant cytotoxic effect against Hela cells (IC50 40.88 ?g/ml). Screening in Hela cells revealed a moderate cytotoxic effect (IC50 80.26?g/ml) of the extract of Rosmarinus officinalis, a mild cytotoxic effect (IC50 148.05 ?g/ml) of the extract of Frangula alnus (originating in Bosnia), and the extract of Frangula alnus (originating in Serbia) did not show active cytotoxicity (IC50 > 200 ?g/ml). The best antiproliferative properties are those of Camellia sinensis, followed by Rosmarinus officinalis, and the least effective was Frangula alnus. As regards geographic origin, the Frangula alnus from Bosnia possessed better antiproliferative effects than Frangula alnus from Serbia.

scholarly journals Antiproliferative effects of Tanaceti partheni, Hypericum perforatum and propolis on HeLa cells

2014 ◽  
Vol 66 (2) ◽  
pp. 705-712 ◽  
Author(s):  
Desanka Cenic-Milosevic ◽  
Zoran Tambur ◽  
S. Ivancajic ◽  
Tatjana Stanojkovic ◽  
Nadja Grozdanic ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cytotoxic Activity ◽  
Hela Cells ◽  
Hypericum Perforatum ◽  
Human Cancer ◽  
Human Cancer Cell ◽  
Polar Solvents ◽  
Antiproliferative Effects ◽  
Human Cancer Cell Lines ◽  
Hypericum Species

Tanaceti partheni, Hypericum perforatum and propolis have been widely used for centuries and are well-documented medicinal plants and natural product. In this study, we investigated the antiproliferative effects of water extracts of ethanolic dry extracts of two different medicinal plants (Tanaceti partheni and Hypericum perforatum) and propolis on HeLa cells. The Tanaceti partheni extract exhibited mild cytotoxic activity. The IC50 was 153.71 ?g/mL. The extract of Hypericum perforatum did not show active cytotoxic activity against HeLa cells (IC50 >200 ?g/mL). Regarding the antiproliferative effects of Hypericum perforatum, our results are not in correlation with the results of other authors, probably because different Hypericum species and different human cancer cell lines were used. The extract of propolis did not show active cytotoxic activity against HeLa cells (IC50 = 1.08 ? 0.01 mg/mL). The weak antiproliferative effect of propolis on HeLa cells is either due to the use of a low concentration of propolis extracted in weakly polar solvents, or the use of propolis collected in the autumn.

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Electron Microscopy of Mycoplasma Pneumoniae

1971 ◽  
Vol 29 ◽  
pp. 258-259 ◽  
Author(s):  
E. S. Boatman ◽  
G. E. Kenny
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Growth Phase ◽  
Hela Cells ◽  
Sulfonic Acid ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Morphological Aspects ◽  
The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

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