scholarly journals Physiological Adaptation and Plant Growth Promoting Functional Traits of Bacillus altitudinis FD48 under In vitro Osmotic Stress

2020 ◽  
pp. 92-98
Author(s):  
Shobana Narayanasamy ◽  
Sugitha Thankappan ◽  
Sivakumar Uthandi
Keyword(s):  
Plant Growth ◽  
Osmotic Stress ◽  
Functional Traits ◽  
Physiological Adaptation ◽  
Bacillus Altitudinis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Impact ◽  
Plant Growth Promoting Traits

To develop an osmotolerant microbe, as a bioinoculant to mitigate drought it is vital to understand the impact of osmotic stress on their growth and plant growth promoting functional traits. The present study was aimed to evaluate the physiological adaptations and plant growth-promoting traits of a phyllosphere bacterium Bacillus altitudinis FD48 under osmotic stress conditions. The FD48 strain isolated from rice (cultivar ADT43) phyllosphere obtained from Biocatalysts laboratory, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore. In vitro bioassay was conducted to evaluate the osmotolerant potentials of FD48. B. altitudinis FD48 grown in LB supplemented with PEG 6000 and grown for 48 hrs. Physiological adaptation to osmotic stress was observed by assessing the osmolytes and free amino acids content produced by FD48 under induced stress. Further the plant growth promoting traits under osmotic stress also asceratined. The growth pattern of FD48 strain decreased with the increase in PEG concentrations. The lower level of osmotic stress enhanced the growth of FD48 but at higher concentration exhibited a decline in growth. Enhanced levels of IAA (25 µg g-1 of protein) and EPS (9.76 mg mg-1 protein) production were recorded in the FD48 strain at lower levels of osmotic stress. Furthermore, an increase in osmotic stress had a deleterious effect on IAA production and ACC deaminase activity while the exopolysaccharide production was enhanced. Growth of FD48 under osmotic stress also increased the accumulation of proline and compatible sugars that will protect the FD48 strain by maintaining the turgor potential of cells and stabilizes the membrane proteins. Hence, the results of our study suggesting that, B. altitudinis FD48 strain has the potential to tolerate osmotic stress and might be used as a newer bio-inoculant for triggering moisture deficit stress resilience in plants.

scholarly journals A COMPARATIVE ANALYSIS OF PLANT GROWTH-PROMOTING TRAITS OF Pseudomonas AND Bacillus STRAINS ISOLATED FROM Lolium perenne RHIZOSPHERIC SOIL IN VOJVODINA (SERBIA) AND THEIR EFFECT ON THE PLANT YIELD

2020 ◽  
Vol 19 (3) ◽  
pp. 37-45
Author(s):  
Dragana Stamenov ◽  
Timea I. Hajnal-Jafari ◽  
Biljana Najvirt ◽  
Snežana Anđelković ◽  
Jelena Tomić ◽  
...  
Keyword(s):  
Plant Growth ◽  
Lolium Perenne ◽  
Biochemical Characterization ◽  
Total Yield ◽  
Forage Crops ◽  
Vitro Assay ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
The Impact

The objective of this work was to do a comparative study of Pseudomonas and Bacillus isolates for their plant growth-promoting (PGP) potential, monitoring the impact of selected isolates on the yield of English ryegrass (Lolium perenne). Isolation, physiological and biochemical characterization, in vitro assay of enzymatic and plant-growth promoting activities of isolates were done. Pseudomonas isolates have been shown to have the ability to use different sources of carbon, to live in the condition of low pH as well as temperature and to produce siderophore. On the other hand, Bacillus isolates have the ability to solubilize phosphate, to produce a greater amount of indol-3-acetic acid (IAA) than Pseudomonas isolates and have an inhibitory effect on the growth of phytopathogenic fungi. In other investigated traits, isolates were similar. The use of Pseudomonas sp. P12 and Bacillus sp. B1 isolates had a positive effect on the plant mass and total yield, which indicate that the use of these isolates can result in a better yield of forage crops.

scholarly journals Isolation and screening of multifunctional rhizobacteria from the selected sites of Madhupur, Narshingdi and Mymensingh, Bangladesh

2015 ◽  
Vol 2 (1) ◽  
pp. 1-8
Author(s):  
Moonmoon Nahar Asha ◽  
Atiqur Rahman ◽  
Quazi Forhad Quadir ◽  
Md Shahinur Islam
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Plant Growth Promoting Rhizobacteria ◽  
N Fixation ◽  
National Botanical Research Institute ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Botanical Research ◽  
Plant Growth Promoting Traits

A laboratory experiment was performed to isolate some native rhizobacteria that could be used as bioinoculants for sustainable crop production. A total of 43 rhizobacteria were isolated from undisturbed plant rhizosphere soils of three different locations of Bangladesh and evaluated their plant growth promoting traits, both direct and indirect. The study has screened out isolates on the basis of their phosphorous solubilization and nitrogen (N) fixation. The phosphate solubilization assay in National Botanical Research Institute of Phosphate (NBRIP) medium revealed that 12 bacterial isolates were able to solubilize tricalcium phosphate and the rhizobacteria M25 showed best performance with a PSI of 3.33 at 5 day. Exactly 47% (20 isolates) of the isolated rhizobacteria were able to grow in N-free Winogradsky’s medium, which is an indication of potential N2-fixers. Among the 20 potential N-fixers, 15 were able to grow within 24 hours of incubation indicating that they are more efficient in Nfixation. The present study successfully isolated and characterized 43 rhizobacteria. Some of these isolated rhizobacteria have potential plant growth promoting traits and are potential plant growth promoting rhizobacteria (PGPR) candidate. Considering all plant growth promoting traits, the isolate F37 was the best followed by M6. However, further experiments are needed to determine the effectiveness of these isolates under in vitro and different field conditions to understand the nature of interaction with the plant and environment.Res. Agric., Livest. Fish.2(1): 1-8, April 2015

scholarly journals De novo genome assembly of Bacillus altitudinis 19RS3 and Bacillus altitudinis T5S-T4, two plant growth-promoting bacteria isolated from Ilex paraguariensis St. Hil. (yerba mate)

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0248274
Author(s):  
Iliana Julieta Cortese ◽  
María Lorena Castrillo ◽  
Andrea Liliana Onetto ◽  
Gustavo Ángel Bich ◽  
Pedro Darío Zapata ◽  
...  
Keyword(s):  
Plant Growth ◽  
De Novo ◽  
Draft Genome ◽  
Ilex Paraguariensis ◽  
Plant Growth Promoting Bacteria ◽  
De Novo Genome Assembly ◽  
Bacillus Altitudinis ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth-promoting bacteria (PGPB) are a heterogeneous group of bacteria that can exert beneficial effects on plant growth directly or indirectly by different mechanisms. PGPB-based inoculant formulation has been used to replace chemical fertilizers and pesticides. In our previous studies, two endophytic endospore-forming bacteria identified as Bacillus altitudinis were isolated from roots of Ilex paraguariensis St. Hil. seedlings and selected for their plant growth-promoting (PGP) properties shown in vitro and in vivo. The purposes of this work were to assemble the genomes of B. altitudinis 19RS3 and T5S-T4, using different assemblers available for Windows and Linux and to select the best assembly for each strain. Both genomes were also automatically annotated to detect PGP genes and compare sequences with other genomes reported. Library construction and draft genome sequencing were performed by Macrogen services. Raw reads were filtered using the Trimmomatic tool. Genomes were assembled using SPAdes, ABySS, Velvet, and SOAPdenovo2 assemblers for Linux, and Geneious and CLC Genomics Workbench assemblers for Windows. Assembly evaluation was done by the QUAST tool. The parameters evaluated were the number of contigs ≥ 500 bp and ≥ 1000 bp, the length of the longest contig, and the N50 value. For genome annotation PROKKA, RAST, and KAAS tools were used. The best assembly for both genomes was obtained using Velvet. The B. altitudinis 19RS3 genome was assembled into 15 contigs with an N50 value of 1,943,801 bp. The B. altitudinis T5S-T4 genome was assembled into 24 contigs with an N50 of 344,151 bp. Both genomes comprise several genes related to PGP mechanisms, such as those for nitrogen fixation, iron metabolism, phosphate metabolism, and auxin biosynthesis. The results obtained offer the basis for a better understanding of B. altitudinis 19RS3 and T5S-T4 and make them promissory for bioinoculant development.

scholarly journals Biocontrol of Sclerotium rolfsii Using Antagonistic Activities of Pseudomonads

2019 ◽  
pp. 1-9
Author(s):  
Sangita Sahni ◽  
Bishun Deo Prasad ◽  
Tushar Ranjan
Keyword(s):  
Calcium Phosphate ◽  
Plant Growth ◽  
Sclerotium Rolfsii ◽  
Antagonistic Activity ◽  
Biocontrol Agents ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Unique Ability ◽  
Plant Growth Promoting Traits

Thirty well-characterized pseudomonad isolates for plant growth-promoting traits were screened for their antagonistic activities against 20 isolates of Sclerotium rolfsii. Out of the 30 pseudomonad isolates, PUR46 was found to be best against all 20 isolates of Sclerotium rolfsii, because of its unique ability to suppress the growth of mycelia as well as the sclerotia formation of most of the S. rolfsii isolates in vitro conditions. In our previous study, PUR46 was also found to be positive for growth promoting traits like phosphorus solubilization and ammonification. The results suggested that expression of one or more of the traits like antagonistic activity against S. rolfsii and solubilization of tri-calcium phosphate may help in controlling the pathogen besides enhancement of plant growth. In this study, our investigations clearly indicate that PGPR isolates PUR 46 may be exploited to be used as potential biocontrol agents against S. rolfsii in agriculture system.

scholarly journals Survey of plant growth promoting and antagonistic traits in winter wheat grain endophytic bacteria

2021 ◽  
pp. 66-72
Author(s):  
Alina Pastoshchuk ◽  
Yuliia Yumyna ◽  
Pavlyna Zelena ◽  
Larysa Skivka
Keyword(s):  
Plant Growth ◽  
Winter Wheat ◽  
Endophytic Bacteria ◽  
Wheat Variety ◽  
Wheat Grain ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

The aim of this work was to isolate endophytic bacteria from wheat grains and to evaluate their plant growth promoting traits (PGPT) as well as an inhibitory effect on P. syringae pv. atrofaciens (McCulloch) growth. Endophytic bacteria were isolated by a culture-dependent protocol from the grains of winter wheat variety of Ukrainian selection Podolyanka with high resistance to syringae. Totally 2.7±0.09 CFU/1 g of dry wheat grain were isolated, ten cultivable bacterial isolates were obtained. Spore-forming bacilli predominated in the wheat grain endophytic community. Gram-negative fermenting and non-fermenting rod-shaped bacteria and Gram-positive cocci were also present. Seven out of ten isolates possessed numerous plant growth promoting traits including phosphate solubilization, oligonitrotrophy, and indolic compound producing. Two isolates possessed antagoniscic activity against syringae in vitro along with plant growth promoting features. According to biochemical profiling and mass-spectrophotometric identification, these two isolates were assigned to Paenibacillus and Brevibacillus genera. These endophytic bacteria can be considered as promising objects for agrobiotechnology. However, more research is needed to confirm their biotechnological potential in planta experiments

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