Evaluation of Nephroprotective Properties of Aqueous and Hydroethanolic Extracts of Crinum scillifolium against Gentamicin Induced Renal Dysfunction in the Albino Rats

Aims: Gentamicin, a widely used aminoglycoside antibiotic, is recognized as possessing significant nephrotoxic potential in human beings. The aim of this study was to determine the protective effect of aqueous and hydroethanolic extracts of Crinum scillifolium on gentamicin induced nephrotoxicity using biochemical approaches and determined the most active extract in rat. Study Design: The leaves of Crinum scillifolium were collected in the district of Me (Côte d’Ivoire). The plant was identified and authenticated by the Department of Botany, Felix Houphouet Boigny University of Abidjan (Côte d’Ivoire). Place and Duration of Study: Analysis of the plant samples was done in pharmacodynamics Biochemistry Laboratory, Felix Houphouet Boigny University and the Laboratory of Organic Chemistry and Natural Substances, Felix Houphouet Boigny University between July and August 2018. Methodology: The extractions were execcuted by macerating 100 g of plant dry powder in 1 liter of distilled water (aqueous extraction) or in 2 liters of water / ethanol mixture (30/70 V / (ethanol extraction). The animals were divided according to weight in seven groups each of six rats. The nephroprotective effects were estimated by comparing the effects of the extracts (100 and 200 mg / kg) to that of vitamin E (250 mg / kg) against gentamicin-induced renal failure by (80 mg / kg). Gentamicin is administered to the animals one hour after treatment with the extracts for seven days. Results: The administration of gentamicin through intraperitoneal route to rats for seven days, resulted in an increase in urea and creatinine concentrations as well as decrease of total protein concentration in the serum. The level of total protein and albumin increased in urine after administration of gentamicin. The treatment of animals suffering from nephrotoxicity with Hydroethanolic and aqueous extracts of Crinum scillifolium would have significantly reduced (P < 0.001 and P < 0.01) biochemical parameters considered as markers of nephrotoxicity. Conclusion: This study proved that the aqueous extract of Crinum scillifolium possesses a nephroprotective activity against gentamicin-induced kidney failure in rats. So aqueous extract can be useful for preventives applications.

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Principes Actifs Et Mise En Evidence Des Sucres Des Aubergines Solanum Anguivi Lam Et Solanum Torvum Récoltées En Côte d’Ivoire

European Scientific Journal ESJ ◽

10.19044/esj.2021.v17n21p33 ◽

2021 ◽

Vol 17 (21) ◽

pp. 33

Author(s):

Dan Chépo Ghislaine ◽

Yao Kouadio ◽

Louis Ban Koffi

Keyword(s):

Cote D'ivoire ◽

Côte D’Ivoire ◽

Solanum Torvum ◽

Cote D’Ivoire ◽

Natural Substances ◽

Côte D'ivoire ◽

Medicinal Value ◽

Couche Mince ◽

Toxic Alkaloids ◽

Valeur Nutritionnelle

En Afrique, de nombreuses espèces végétales ne sont pas suffisamment exploitées, malgré leurs valeurs comestibles indéniables. Parmi ces plantes figurent les aubergines amères Solanum anguivi Lam et Solanum torvum communément appelées "gnagnan" en Côte d'Ivoire. Les baies de ces légumes-fruits sont séchées et analysées selon des méthodes standard et conventionnelles. Le tri phytochimique a révélé que S. torvum contient plus de stérols, polyphénols, quinones et saponines par rapport à S. anguivi Lam. Aucune des deux aubergines ne contient d'alcaloïdes toxiques pour l'alimentation. En chromatographie sur couche mince, les sucres détectés sont le glucose, le xylose, l'arabinose et le saccharose. Cependant, le saccharose est remplacé par le fructose dans les baies de S. torvum. Ainsi, ces deux espèces pourraient être de bonnes substances naturelles avec une valeur nutritionnelle et médicinale intéressante. In Africa, many plant species are not sufficiently exploited, despite their undeniable edible values. Among these plants are the bitter eggplants Solanum anguivi Lam and S. torvum commonly called "gnagnan" in Côte d’Ivoire. Berries of these fruit vegetables are dried and analyzed according to standard and conventional methods. Phytochemical sorting revealed that S. torvum contains more sterols, polyphenols, quinones and saponins compared to S. anguivi Lam. Neither eggplant contains toxic alkaloids in the diet. In thin layer chromatography, the sugars detected are glucose, xylose, arabinose and sucrose. However, sucrose is replaced by fructose in the berries of S. torvum. Thus, these two species could be good natural substances with interesting nutritional and medicinal value.

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Comparative Study of the Chemical Composition and Antioxidant Capacity of Leaves, Stems and Roots of Alchornea cordifolia (Schumach. & Thonn.) Müll. Arg.

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i430385 ◽

2021 ◽

pp. 65-75

Author(s):

Emmanuel N’Dri Koffi ◽

Olivier Hugues Alain N’Guessan ◽

Philippe Kessé N’Da ◽

Ismael Sanga Ouattara ◽

Séraphin Kouakou Konan ◽

...

Keyword(s):

Chemical Composition ◽

Antioxidant Capacity ◽

Aqueous Extract ◽

Cote D'ivoire ◽

Côte D’Ivoire ◽

Industrial Processes ◽

Root Extract ◽

Phytochemical Screening ◽

Cote D’Ivoire ◽

Côte D'ivoire

Aims: This work aims to compare the chemical composition and the antioxidant capacity of the leaves, stems and roots of Alchornea cordifolia. Study design: The leaves, stems and roots of Alchornea cordifolia were harvested during the month of August 2020 in the botanical garden of the University Jean Lorougnon Guede in Daloa. After that, they were dried in the shade for 17 days. Subsequently, they were crushed and kept in jars in order to be sent to the Laboratory of Industrial Processes, Synthesis, Environment and New Energies (LAPISEN) of Yamoussoukro (Côte d'Ivoire), for analyzes. Place and duration of study: This study was carried out during september to december 2020 in Laboratory of Industrial Processes, Synthesis, Environment and New Energies (LAPISEN) of Yamoussoukro (Côte d'Ivoire) Methodology: Physicochemical parameters (pH, ° Brix, MS) and the mineral contents of leave, stem and root aqueous extracts were determined by classic methods. Then, phytochemical screening are carried out by TLC analysis followed by spectrophotometric assay of total polyphenols, total flavonoids and antioxidant activity. Results: The results showed that the aqueous extract from A. cordifolia leaves, stems and roots are all acidic (pH ≤ 5.33). However, these extracts contain a low level of dry matter. The measured minerals (Na, Ca, Mg, Mn, K, Fe; Cu and Zn) are present in these extracts at varying concentrations. The leaf extract is richer in Fe (8.28 ppm), Ca (1.71 ppm), Mn (1.17 ppm) and K (0.55 ppm). In contrast, the root extract contains more Na (18.38 ppm), Cu (0.75 ppm) and Mg (0.34 ppm). As for the phytochemical screening revealed the presence of coumarins, flavonoids, sterols and terpenes, in the aqueous extract of all organs, except tannins, absent in the roots. However, the leaves have the highest content of polyphenols (256.67 mg.g-1 GAE) and flavonoids (92.75 mg.g-1 QE), as well as the best antioxidant capacity (204.23 µmol.L -1 TE). Conclusion: The leaves, stems and roots of A. cordifolia contain practically the same chemical compounds with a few exceptions. However, the difference lies in the concentration of these compounds in the different extracts.

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