Immunohistochemical Characterization of Genes Expressed in Leiomyoma using Ki67 and P53 in Patient Attending Niger Delta University Teaching Hospital

Objectives: This study aimed to investigate the expressions of p53 and ki67 genes in the characterization of leiomyomas. Materials: Fifty (50) Paraffin embedded tissue blocks were selected from the department of Histopathology, Niger Delta University Teaching Hospital, Okolobri from January 2011 to August 2019. The histological characterization and morphological evaluation were defined using the routine Hematoxylin and Eosin staining method. Immunostaining was done for p53 and ki67 using the manufacturers antibodies. Results: A direct correlation between age and the expression of the p53 gene was also made in this study. It was observed that in patients within the ages of 50-59 and 60-69, there was markedly no expression of the p53 gene whereas in patients within the ages of 20-29, there was still some level of expression (20%) and ages 30-39 (80%) and finally 40-49 (10%). There was no expression (0.0%) of Ki-67 in uterine leiomyomas across all age groups. Conclusion; This study therefore has suggested that based on the percentage of expression, it can rightly be inferred that the p53 gene is a good indicator in the immune-histochemical characterization of leiomyomas as it has good sensitivity and good prognosis as opposed to Ki67.

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Experience working with Nurse Anesthetists’ as Non-Physician Anesthesia Providers in a temporary Semi-Urban Niger Delta University Teaching Hospital, Okolobiri and review of the literature.

IOSR Journal of Dental and Medical Sciences ◽

10.9790/0853-1233640 ◽

2013 ◽

Vol 12 (3) ◽

pp. 36-40

Author(s):

Dr. Fente BG ◽

Keyword(s):

Teaching Hospital ◽

Niger Delta ◽

Nurse Anesthetists ◽

University Teaching ◽

Review Of The Literature ◽

Anesthesia Providers

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A Longitudinal Analysis of Methicillin-ResistantStaphylococcus aureusin a Hong Kong Teaching Hospital

Infection Control and Hospital Epidemiology ◽

10.1086/502362 ◽

2004 ◽

Vol 25 (2) ◽

pp. 126-129 ◽

Cited By ~ 10

Author(s):

Margaret Ip ◽

D. J. Lyon ◽

F. Chio ◽

A. F. Cheng

Keyword(s):

Hong Kong ◽

Teaching Hospital ◽

Dna Typing ◽

Type A ◽

University Teaching ◽

Pfge Type ◽

Type B ◽

Isolation And Characterization ◽

Surgical Units

AbstractObjectives:To review the incidence and trends of MRSA during a 12-year (1989-2000) period at a university teaching hospital and the relationship between strain distribution by antibiogram and molecular typing.Design:Retrospective review of laboratory-based surveillance records on MRSA isolation and characterization of strains by antimicrobial susceptibility and PFGE. A patient episode was counted at the time when MRSA was first isolated.Setting:A 1,350-bed university teaching hospital in Hong Kong.Patients:Those with clinical isolates of MRSA.Results:During 1989 to 2000, the hospital recorded 1,203,175 deaths and discharges (D&D) and encountered 5,707 patient episodes of new MRSA isolation. The overall incidence of patient episodes of MRSA was 0.47/100 D&D. In 1989, the incidence was 0.81/100 D&D and fell to a low of 0.33/100 D&D in 1995, but then rose to 0.50/100 D&D in 2000. Antibiogram and DNA typing identified 5 major types. PFGE type A constituted 68% (211/312) of isolates and was present throughout the 12-year period. PFGE type B constituted 13% (40/312) of isolates and was only present from 1995 to 2000. These isolates form a distinct clone and had unique antibiotic resistance profiles.Conclusions:The study showed the establishment of a dominant MRSA clone (PFGE type A group) in the intensive care, medical, and surgical units and the appearance of a new MRSA strain in 1995 (PFGE type B), which partly explained the rise in incidence of MRSA cases and a disproportionate rise in MRSA bacteremia from 1995 to 2000.

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The Histopathologic Patterns of Intracranial Neoplasms in the Lagos University Teaching Hospital (LUTH), Idi-Araba, Lagos, Nigeria: A 10-Year Retrospective Study, January 2008 to December 2017

American Journal of Clinical Pathology ◽

10.1093/ajcp/aqz122.009 ◽

2019 ◽

Vol 152 (Supplement_1) ◽

pp. S120-S121

Author(s):

Kasiemobi Uchime ◽

Luqman Adebayo ◽

Charles Anunobi

Keyword(s):

Teaching Hospital ◽

Pituitary Adenomas ◽

Age Groups ◽

University Teaching ◽

Geographical Differences ◽

Intracranial Neoplasms ◽

Female Ratio ◽

Intracranial Neoplasm ◽

Male To Female ◽

Age And Sex

Abstract Objectives Intracranial neoplasms have distinct diagnostic histologic features and some are common in certain gender and age groups. The most common intracranial neoplasm worldwide is meningioma, followed by gliomas, most especially astrocytic tumors, and then pituitary adenomas. There are geographical differences in the pattern of occurrence of intracranial neoplasms. Few studies have been done in Nigeria to demonstrate the pattern of occurrence, age, and sex distributions of these neoplasms. The aim for this study is to establish the pattern of occurrence with age and sex distribution of different histologic types of intracranial neoplasm in our environment. This study may help portray the health burden of these tumors and aid in epidemiological studies. Methods A total of 296 patients (165 females, 131 males) diagnosed with intracranial neoplasms between January 2008 and December 2017 at Lagos University Teaching Hospital (LUTH), Idi-Araba, Lagos, Nigeria, were retrospectively analyzed. Patients’ data were retrieved from the archives of the Department of Anatomic and Molecular Pathology, LUTH, Idi-Araba, Lagos. Histologic patterns with age and gender distribution were noted. The data obtained were analyzed with SPSS version 23. Results Majority of the patients diagnosed with intracranial neoplasm were between 41 and 50 years of age. The most frequently diagnosed intracranial neoplasm at LUTH within the study period was meningioma (105 cases, median age of 42 years, male to female ratio of 3:7), followed by pituitary adenoma (78 cases, median age of 47 years, male to female ratio of 3:2), and then gliomas (66 case), most especially the astrocytic and oligodendroglial tumors (median age of 37 years, male to female ratio of 2:3). Conclusion The result of the study shows that the pattern of occurrence of primary intracranial neoplasms in our environment is different from that in Caucasians, with meningiomas being the most common, followed by pituitary adenomas and then gliomas.

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OVERVIEW OF ULTRASONOGRAPHIC OCULAR FINDINGS IN JOS UNIVERSITY TEACHING HOSPITAL

10.36106/1426571 ◽

2019 ◽

pp. 1-3

Author(s):

Salaam Aj ◽

Danjem Sm* ◽

Salaam Aa ◽

Iyua Ko ◽

Kolade-yunusa Ho

Keyword(s):

Teaching Hospital ◽

Structural Integrity ◽

Age Groups ◽

Vitreous Hemorrhage ◽

University Teaching ◽

Common Finding ◽

Direct Visualization ◽

Non Invasive ◽

Ocular Ultrasound ◽

One Year

CONTEXT: Ocular ultrasound, also known as ocular echography or B-Scan, is a quick, non-invasive test routinely used in clinical practice to assess the structural integrity and pathologies of the eye. It can provide additional information not readily obtained by direct visualization of ocular tissues, and it is particularly useful in patients with pathologies that prevents or obscures ophthalmoscopy, e.g. large corneal opacities, dense cataracts, or vitreous hemorrhage. The gelatinous composition of the eye makes it an ideal organ for ultrasound. While direct visualization with fundoscopy is limited due to variable provider skill, ultrasound provides reproducible, non-invasive visualization of the anterior chamber, globe, and posterior elements of the eye. AIM: The aim of the study is to audit all the ocular scans done in the Department of Radiology over a one year period, to ascertain indications and findings with respect to age groups and sex. METHODOLOGY: This was a retrospective study carried out in the Department of Radiology, Jos University Teaching Hospital, over a period of one year, between January, 2013 and January 2014. RESULTS: A total of 99 patients were scanned over the period, 53 of which were males and 46 females. Traumatic cataract and poor vision were the most common indications for ocular B-Scan. The most common finding was normal scan, while intra-ocular foreign body was the least common. CONCLUSION: Ocular ultrasonography is a safe imaging tool in evaluating patients with ocular diseases. Its utilization can be of great help in the management of ophthalmology cases.

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