scholarly journals Application of Platelet Rich Fibrin, Platelet Rich Plasma, Plasma Rich Growth Factors in Different Fields of Dentistry: An Overview

2020 ◽  
pp. 12-23
Author(s):  
Geetanjali Singh ◽  
Bhawna Kumari ◽  
Harangad Singh Grover ◽  
Akriti Mahajan ◽  
Farhat Jabeen ◽  
...  
Keyword(s):  
Growth Factors ◽  
Soft Tissue ◽  
Plasma Proteins ◽  
Bone Growth ◽  
Platelet Rich Plasma ◽  
Platelet Rich Fibrin ◽  
Soft Tissue Regeneration ◽  
Tissue Healing ◽  
Transversal Field ◽  
Potential Use

A huge number of studies have demonstrated that platelet-rich preparations applied to surgical sites, injuries, or wounds are effective way to promote soft tissue healing and bone growth. The potential use of  platelet rich preparations like Platelet - rich plasma and Platelet - rich fibrin are new boon to dentistry termed under the general acronyms PRP (Platelet-Rich Plasma) or PRF (Platelet-Rich Fibrin), is an important current transversal field of research across many fundamental and clinical disciplines.The third important is PRGF.  Plasma Rich in Growth Factor (or PRGF) is a type of plasma enriched of proteins and circulating growth factors able to aid the bone and soft tissue regeneration. PRGF includes plasma proteins and coagulative factors and is then more valuable compared to PRP. There are many scientific literature which cover one or other concise aspect of platelet rich preparation. This review aims at covering all basic definitions, key element, history, preparation, differences between PRF and PRP and applications in a simplified manner.

scholarly journals Changes in the activity of ovine blood-derived macrophages stimulated with antimicrobial peptide extract (AMP) or platelet-rich plasma (PRP)

2019 ◽  
Vol 63 (2) ◽  
pp. 235-242
Author(s):  
Joanna Wessely-Szponder ◽  
Tomasz Szponder ◽  
Ryszard Bobowiec ◽  
Joanna Michalska
Keyword(s):  
Antimicrobial Activity ◽  
Growth Factors ◽  
Soft Tissue ◽  
Platelet Rich Plasma ◽  
Innate Immune ◽  
Ros Generation ◽  
Soft Tissue Healing ◽  
Tissue Healing ◽  
Functional Features ◽  
Stimulation Of

AbstractIntroduction: Antimicrobial peptides (AMP) are a large group of innate immune effectors, which apart from antimicrobial activity show immunomodulative properties. Platelet-rich plasma (PRP) is a source of autologous growth factors and is used for stimulation of bone and soft tissue healing. The purpose of this study was to assess the influence of PRP and AMP extract on ovine monocyte-derived macrophage cultures. Material and Methods: The study was conducted on ovine macrophages (Mfs) previously stimulated with LPS or dexamethasone and then with preparations of PRP or AMP. Following activation of the Mfs their morphological and functional features were assessed. Results: The study revealed pro-inflammatory influence of both examined preparations on Mfs cultures on the basis of morphology, ROS generation and arginase activity. Both preparations enhanced the pro-inflammatory response of cultured Mfs. Conclusion: This activity may intensify the antimicrobial action of Mfs, however, in cases of excessive and prolonged inflammation the use of these preparations should be limited.

scholarly journals Effects of Leukocyte-Platelet-Rich Fibrin (L–PRF) on Pain, Soft Tissue Healing, Growth Factors, and Cytokines after Third Molar Extraction: A Randomized, Split-Mouth, Double-Blinded Clinical Trial

2021 ◽  
Vol 11 (4) ◽  
pp. 1666
Author(s):  
Madelaine Torres da Silva ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
Rafael Coutinho Mello-Machado ◽  
Pietro Montemezzi ◽  
Renata de Lima Barbosa ◽  
...  
Keyword(s):  
Growth Factors ◽  
Postoperative Pain ◽  
Soft Tissue ◽  
Third Molar ◽  
Local Concentration ◽  
Third Molars ◽  
Soft Tissue Healing ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Double Blinded

This study assessed the effects of leukocyte-platelet-rich fibrin (L–PRF) on soft tissue healing and the correlation with the local concentration of growth factors (GF) and cytokines in the dental socket of lower third molars. Forty lower-third molars (20 participants) were included in this randomized, double-blinded, split-mouth study. After extractions, randomized sides received alveolar filling with L–PRF on one side and a natural blood clot on the other side. The pain was assessed for up to seven days and soft tissue healing (Landry index) for 14 days post-extraction. Swabs were collected from the surgical sites for GF and cytokine assessment by flow luminometry. Participants reported lower postoperative pain on the sides grafted with L–PRF, which also presented increased tissue healing scores (p < 0.05). There were increased levels of all GFs and several cytokines at the L–PRF site at day one, while vascular endothelial growth factor (VEGF), IL–10, and IL1–RA remained higher throughout for seven days (p < 0.05). VEGF concentration at L–PRF sites correlated positively with the participants’ blood platelet content (ρ = 0.769). PDGF correlated negatively with pain experience on days 2 and 3, and positively with soft tissue healing scores, while FGFb presented a weak correlation with a reduction of pain on day 3. The use of L–PRF improves the soft tissue healing process and decreases postoperative pain after the third molar extractions, which correlates with an increase in the local concentration of growth factors such as PDGF and FGFb.

scholarly journals Obtention of injectable platelets rich-fibrin (i-PRF) and its polymerization with bone graft: technical note

2015 ◽  
Vol 42 (6) ◽  
pp. 421-423 ◽  
Author(s):  
Carlos Fernando de Almeida Barros Mourão ◽  
Helder Valiense ◽  
Elias Rodrigues Melo ◽  
Natália Belmock Mascarenhas Freitas Mourão ◽  
Mônica Diuana-Calasans Maia
Keyword(s):  
Bone Graft ◽  
Platelet Rich Plasma ◽  
Technical Note ◽  
Platelet Concentrates ◽  
Soft Tissue Healing ◽  
Liquid Form ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Autologous Platelet ◽  
Injectable Form

The use of autologous platelet concentrates, represent a promising and innovator tools in the medicine and dentistry today. The goal is to accelerate hard and soft tissue healing. Among them, the platelet-rich plasma (PRP) is the main alternative for use in liquid form (injectable). These injectable form ofplatelet concentrates are often used in regenerative procedures and demonstrate good results. The aim of this study is to present an alternative to these platelet concentrates using the platelet-rich fibrin in liquid form (injectable) and its use with particulated bone graft materials in the polymerized form.

scholarly journals Effect of Platelet-Rich Plasma and Amniotic Membrane in Patients with Rotator Cuff Repair

2018 ◽  
Vol 39 ◽  
pp. 98-102
Author(s):  
Heri Suroto ◽  
Ameria Pribadi ◽  
Dwikora Novembri Utomo ◽  
Ferdiansyah Mahyudin ◽  
Lukas Widhiyanto
Keyword(s):  
Soft Tissue ◽  
Rotator Cuff ◽  
Pain Score ◽  
Amniotic Membrane ◽  
Rotator Cuff Repair ◽  
Platelet Rich Plasma ◽  
Soft Tissue Healing ◽  
Disability Score ◽  
Tissue Healing ◽  
Cuff Repair

Rotator cuff disorders are the most common source of shoulder problems, ranging from mild strain to massive tears. Platelet-rich plasma (PRP), an autologous blood with platelets concentration above baseline values represents a source of multiple growth factors that promotes tissue repair. This review examines the potential of using PRP to augment rotator cuff repair. Reporting 4 patients with impingement syndrome and supraspinatus tear who underwent decompression acromioplasty and supraspinatus repair augmented with platelet-rich plasma and amniotic membrane. An evaluation was made 3-24 months postoperative using Shoulder Pain and Disability Index (SPADI). Average preoperative pain score is 64%, disability score 54.58%, and total score 58.19%. Average postoperative pain score is 0%, disability score 0.42%, and total score 0.26% (Minimum Detectable Change at 90% confidence for pain score is 18%, disability score 13%, and total score 11%). This result is consequent with research by Luoay Fallouh, stating that improvement is caused by growth factor effects in platelet-rich plasma which promotes soft tissue healing. It can be concluded that platelet-rich plasma and amniotic membrane have promising effects to enhance soft tissue healing in patients with rotator cuff syndrome. Shoulder function is restored with no limitation on daily activity and pain is no longer present.

scholarly journals Concentrated Growth Factors vs. Leukocyte-and-Platelet-Rich Fibrin for Enhancing Postextraction Socket Healing. A Longitudinal Comparative Study

2020 ◽  
Vol 10 (22) ◽  
pp. 8256
Author(s):  
Marco Mozzati ◽  
Giorgia Gallesio ◽  
Margherita Tumedei ◽  
Massimo Del Fabbro
Keyword(s):  
Growth Factors ◽  
Oral Surgery ◽  
Platelet Rich Fibrin ◽  
Tissue Healing ◽  
Significant Difference ◽  
Postoperative Symptoms ◽  
Concentrated Growth Factors ◽  
Socket Healing ◽  
And Control ◽  
Tooth Extractions

Platelet concentrates (PCs) have been used for over 20 years in dentistry, as an adjunct to oral surgery procedures, to improve hard and soft tissue healing and control postoperative symptoms. Among various PCs, Leukocyte and Platelet-Rich Fibrin (L-PRF) has become very popular due to its excellent cost-effectiveness ratio, and to the simple preparation protocol, but comparative clinical studies with other PCs are lacking. The aim of this split-mouth cohort study was to evaluate the effect of Concentrated Growth Factors (CGF), a recently introduced PC, as compared to L-PRF for enhancing post-extraction socket healing. Methods: Patients in need of bilateral tooth extractions were included. Each side was treated with either CGF or L-PRF. Pain, socket closure and healing index were the main outcomes. Results: Forty-five patients (24 women), aged 60.52 ± 11.75 years (range 37–87 years) were treated. No significant difference in outcomes was found, except for Pain at day 1 (p < 0.001) and socket closure in the vestibulo-palatal/lingual dimension at day 7 post-extraction (p = 0.04), both in favor of CGF. Conclusions: based on the present results, CGF proved to be as effective and safe as L-PRF, representing a valid alternative option for improving alveolar socket healing and reducing postoperative discomfort.

scholarly journals The effectiveness of platelet concentrations in periodontal surgeries

2018 ◽  
Vol 6 (2) ◽  
pp. 61 ◽  
Author(s):  
Ronad Al-Azem ◽  
Neveen Ali ◽  
Diana Mostafa
Keyword(s):  
Case Report ◽  
Growth Factors ◽  
Bone Graft ◽  
Tissue Regeneration ◽  
Platelet Rich Plasma ◽  
Research Process ◽  
Platelet Concentrates ◽  
Platelet Rich Fibrin ◽  
Barrier Membrane ◽  
Concentrated Growth Factors

Platelets release several growth factors which stimulate tissue regeneration. Several techniques for platelet concentrates such as platelet rich plasma (PRP), plasma rich in growth factors (PRGF), platelet rich fibrin (PRF) and concentrated growth factors (CGF) have been introduced in dental surgeries for the prevention of hemorrhage and acceleration of tissue regeneration. However, a fabricating growth factors-enriched bone graft matrix which is called “sticky bone” has been demonstrated to provide stabilization in bony defects. In this article, we presented the method of preparing and utilizing CGF and sticky bone and evaluate the effect of CGF mixed with bone graft and CGF barrier membrane in periodontal surgeries.Methodology: We used websites such as PubMed, Scopus, and ISI Web of knowledge to get related articles about this subject. The research process involved specific key words " concentrated growth factor”- “Platelet rich fibrin”- growth factors-enriched bone graft” - “sticky bone” to find more articles which published from 2007 to March 2018.Results: We reviewed 48 articles, 43 articles were excluded. Only five articles have been conducted. Original human studies and case report were included.Conclusion: We concluded that the use of sticky bone and CGF is effective in bone grafting and implant.  

Spontaneous Bilateral Ankle and Midfoot Sepsis in a Nonimmunocompromised Patient

2006 ◽  
Vol 96 (2) ◽  
pp. 139-147
Author(s):  
Thomas S. Roukis ◽  
Jeffrey R. Baker ◽  
Breck Tiernan
Keyword(s):  
Soft Tissue ◽  
Bone Growth ◽  
Platelet Rich Plasma ◽  
Growth Stimulation ◽  
Lower Leg ◽  
Split Thickness Skin Graft ◽  
Allogeneic Bone ◽  
Thickness Skin ◽  
Autologous Platelet Rich Plasma ◽  
The Right

We describe a 70-year-old nonimmunocompromised woman with spontaneous bilateral ankle and midfoot sepsis and a deep-space abscess of the right lower leg. Salvage of both limbs was achieved by aggressive bilateral soft-tissue and osseous debridement, including a four-compartment fasciotomy of the right lower leg, antibiotic-loaded polymethyl methacrylate bone cement implantation, delayed allogeneic bone grafting of the osseous defects impregnated with autologous platelet-rich plasma bilaterally, and external fixation immobilization, implantable bone growth stimulation, and split-thickness skin graft coverage of the right lower leg, ankle, and foot. Osseous incorporation of the bone grafts bilaterally occurred 8 weeks after surgery. No soft-tissue or osseous complications occurred during the postoperative period or at 18-month follow-up except for arthrofibrosis in the right ankle; there was no evidence of recurrent abscesses, sequestrum, or wound-related problems. A review of the literature regarding bilateral pedal sepsis and the techniques used for limb salvage in this patient are presented in detail. (J Am Podiatr Med Assoc 96(2): 139–147, 2006)

Extraction Socket Management Utilizing Platelet Rich Fibrin: A Proof-of-Principle Study of the “Accelerated-Early Implant Placement” Concept

2016 ◽  
Vol 42 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Georgios A. Kotsakis ◽  
Foteini Boufidou ◽  
James E. Hinrichs ◽  
Hari S. Prasad ◽  
Michael Rohrer ◽  
...  
Keyword(s):  
Growth Factors ◽  
Treatment Time ◽  
Primary Stability ◽  
Clinical Results ◽  
Recipient Site ◽  
Platelet Rich Fibrin ◽  
Osteoblastic Activity ◽  
Implant Placement ◽  
Tissue Healing ◽  
Proof Of Principle

Dental implants are widely accepted as the golden standard for the rehabilitation of an edentulous site following the extraction of a tooth. The ideal time for implant placement is dependent on the time required for partial or complete tissue healing and the adequacy of socket dimensions. The use of autologous growth factors is a promising new concept that aids clinicians in minimizing treatment time and increasing patient satisfaction. The purpose of this paper is to introduce a protocol for “accelerated-early” implant placement. In this protocol, platelet rich fibrin is employed to accelerate soft and hard tissue healing and to provide a better-healed recipient site for accelerated, early implant placement. Histological analysis revealed that at 6 weeks postextraction, the application of our approach resulted in delicate newly formed bone showing intense osteoblastic activity surrounded by connective tissue as well as areas of mineralized tissue. The present study is a proof-of-principle study of the acceleration of the physiologic postextraction healing sequelae with the use of autologous growth factors. The accelerated-early implant placement concept is a bioengineered protocol that may aid clinicians to achieve increased primary stability, by placing implants in ridges in an advanced stage of bone healing, while offering patients the benefits associated with early implant placement. Controlled studies are warranted to verify the reproducibility of this treatment concept and identify specific indications where the use of the presented technique can lead to significant clinical results.

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