scholarly journals Phytochemical Screening and In-vitro Free Radical Scavenging Activity of Unani Formulation Habb-e-Asgand

2021 ◽  
pp. 1-7
Author(s):  
Ansari Imtiyaz Ahmed Tufail Ahemad ◽  
Qazi Majaz Ahamad Aejazuddin ◽  
G. J. Khan
Keyword(s):  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Hydroxyl Radical ◽  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Hydroxyl Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Aim: In the present work we aimed to perform phytochemical Screening and In-vitro free radical scavenging activity of Unani formulation Habb-e-asgand. Study Design: The present work was designed to determine the total antioxidant capacity (TAC), Ferrous reducing antioxidant capacity assay (FRAC), DPPH radical scavenging assay, hydroxyl radical scavenging activity, and nitric oxide scavenging assay. Place and Duration of the Study: The present work has been carried out at Ali-allana College of Pharmacy, Akkalkuwa, from February-2020 to November-2020. Methodology: Habb-e-asgand (HEA), a Unani polyherbal drug used in arthritis, gout, and joint pain, is a blend of many herbal medicinal plants. Scientific attempts to test and validate its effectiveness are scarce. The selected Unani formulation was evaluated for DPPH radical scavenging activity, hydroxyl radical scavenging activity, and nitric oxide scavenging assay. The given formulation also evaluated the total antioxidant capacity and the reducing antioxidant capacity of ferrous metals. Results: It was found that DPPH radical scavenging activity of HEA and BHT at a concentration of 100 μg/ml was 95±0.74 and 59±0.94 μg/ml. Hydroxyl radical scavenging activity of HEA, at the concentration of 100μg/ml, was 55±0.64, whereas at the same concentration catechin was 63±0.84 μg/ml. The IC50 value for nitric acid scavenging activity was found to be 49.60±1.57 for HEA, and 186.34 ± 3.28 µg/ml for ascorbic acid. The result obtained from TAC and FRAC activity demonstrates that HEA possessed the highest TAC and FRAC values in comparison to standards catechin and ascorbic acid. Conclusion: From the above results it was concluded that HEA has a potent antioxidant activity. HEA showed moderate to high TAC and FRAC in dose-dependent manner compared to standards catechin and ascorbic acid. HEA shows dose-dependent DPPH and hydroxyl radical scavenging activity. Nitric oxide generation was observed after the incubation of solutions of sodium nitroprusside in PBS at 25°C for 150 min. HEA effectively reduced the generation of nitric oxide.

IN VITRO ANTIOXIDANT AND ALPHA-AMYLASE, ALPHA-GLUCOSIDASE INHIBITORY ACTIVITY OF FLOWERS OF CALOTROPIS GIGANTEA

INDIAN DRUGS ◽  
2014 ◽  
Vol 51 (10) ◽  
pp. 38-42
Author(s):  
N. K Choudhary ◽  
◽  
J Dwivedi ◽  
S Sharma
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Antidiabetic Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The present investigations were carried out to evaluate the in vitro antioxidant as well as antidiabetic activity of flowers of Calotropis gigantea. Different extracts (petroleum ether, chloroform and ethanolic extract) were prepared using successive solvent extraction method (soxhlet) and screened for its in vitro antioxidant activity using Diphenyl picryl hydrazyl (DPPH) radical scavenging activity, ABT S radical cation decolorization assay and nitric oxide (NO) radical scavenging activity and IC50 were calculated. In vitro antidiabetic activity was studied using α – amylase and α – glucosidase inhibitory assay. Chloroform extract, among the three extracts (i.e. petroleum ether, chloroform and ethanolic), showed maximum antioxidant activity with IC50 value of 151.23µg/ml, 73.56 µg/ml and 158.92µg/ml against DPPH radical scavenging activity, ABTS radical cation decolorization assay and nitric oxide (NO) radical scavenging activity respectively. The chloroform extract produced a significant in vitro antidiabetic activity with IC50 of 52.3µg/ml 18.2µg/ml against α – amylase and α – glucosidase enzymes but less inhibitory effect than standard acarbose.

Chemical Composition of Essential Oil and Antioxidant Activities of Curcuma petiolata Roxb. Rhizomes

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan
Keyword(s):  
Ascorbic Acid ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Etoac Extract ◽  
Dpph Radical Scavenging

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction

scholarly journals Improving the Berry Quality and Antioxidant Potential of Flame Seedless Grapes by Foliar Application of Chitosan–Phenylalanine Nanocomposites (CS–Phe NCs)

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2287
Author(s):  
Gholamreza Gohari ◽  
Elnaz Zareei ◽  
Muhittin Kulak ◽  
Parisa Labib ◽  
Roghayeh Mahmoudi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Grape Berries ◽  
Pal Activity ◽  
Dpph Radical Scavenging

The production and sustainability of grape berries with high quality and health-promoting properties is a major goal. In this regard, nano-engineered materials are being used for improving the quality and marketability of berries. In this study, we investigated the potential role of chitosan–phenylalanine nanocomposites (CS–Phe NCs) in improving the quality of Flame Seedless (Vitis vinifera L.) grape berries, such as titratable acidity (TA), pH, total soluble solids (TSS), ascorbic acid, total phenolics, total flavonoids, anthocyanin, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, and phenylalanine ammonia-lyase (PAL) activity. In this context, grape berries collected in two growing seasons (2018–2019) were screened. Regarding the experimental design, the treatments included chitosan at a 0.5% concentration (CS 0.5%), phenylalanine at 5 mM and 10 mM concentrations (Phe 5 mM and Phe 10 mM), and chitosan–phenylalanine nanocomposites (CS–Phe NCs) at 5 mM and 10 mM concentrations. The lowest TA was recorded in grape berries treated with CS–Phe NCs with a 10 mM concentration. However, treatments enhanced with TSS, which reached the highest value with 10 mM of CS–Phe NCs, were reflected as the highest ratio of TSS/TA with 10 mM of CS–Phe NC treatment. Nanocomposites (NCs) also increased pH values in both study years compared to the control. Similarly, the ascorbic acid and total phenolic content increased in response to NP treatment, reaching the highest value with 5 mM and 10 mM of CS–Phe NCs in 2018 and 2019, respectively. The highest flavonoid content was observed with 5 mM of CS–Phe NCs in both study years. In addition, the anthocyanin content increased with 5 and 10 mM of CS–Phe NCs. PAL activity was found to be the highest with 5 mM of CS–Phe NCs in both study years. In addition, in accordance with the increase in PAL activity, increased total phenolics and anthocyanin, and higher DPPH radical scavenging activity of the grapes were recorded with the treatments compared to the control. As deduced from the findings, the coating substantially influenced the metabolic pathway, and the subsequent alterations induced by the treatments were notably appreciated due to there being no adverse impacts perceived.

scholarly journals Variability in Anthocyanins, Phenolic Compounds and Antioxidant Capacity in the Tassels of Collected Waxy Corn Germplasm

Agronomy ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 158 ◽  
Author(s):  
Prakasit Duangpapeng ◽  
Kamol Lertrat ◽  
Khomsorn Lomthaisong ◽  
Marvin Paul Scott ◽  
Bhalang Suriharn
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Variance ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Bioactive Properties ◽  
Waxy Corn ◽  
Dpph Radical Scavenging

Corn tassel is a valuable co-product and an excellent source of phytochemicals with bioactive properties. The information on the genetic diversity in the tassel properties of waxy corn germplasm is important for creating new varieties that can have the potential for the commercial production of tassels as a co-product. Therefore, the objective of this study was to evaluate the potential of corn tassels in a set of waxy corn germplasm for the extraction of phenolic compounds with an antioxidant activity. The experiment was carried out under field conditions in the rainy season 2017 and the dry season 2017/2018. Fifty waxy corn genotypes were evaluated. Data were collected for the total anthocyanin content (TAC), total phenolic content (TPC) and the antioxidant activity was determined by the 2,2-diphenyl−1-picrylhydrazyl (DPPH) radical scavenging activity and Trolox equivalent antioxidant capacity (TEAC) assays. The season (S) had small effect on all of the parameters, accounting for 0.2–8.7% of the total variance. The genotype (G) was the largest variance component in the TAC and DPPH radical scavenging activity, accounting for 83.5–97.5% of the total variance. The G and S × G interaction contributed approximately equally to the total variance in the TPC and TEAC. Based on the TAC, TPC and antioxidant capacity variation, the genotypes were classified into seven groups. The tassels of corn genotypes belonging to three of these clusters (clusters E, F and G) had high levels of phytochemicals along with an antioxidant capacity. A significant correlation coefficient was found between the TAC and DPPH (r = 0.70 **). The TPC showed a moderate relationship with the DPPH and TEAC assays (r = 0.60 ** and 0.76 **, respectively). The information obtained from this study can be used for germplasm management and waxy corn breeding for enhancing levels of bioactive properties in waxy corn tassels.

scholarly journals Chemical and Antioxidant Properties of Broccoli Growing in Bangladesh

1970 ◽  
Vol 9 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Ismet Ara Jahan ◽  
M Mostafa ◽  
Ishrat Nimmi ◽  
M Hemayet Hossain ◽  
Moinul Ahsan ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ferrous Ion ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Chelating Ability ◽  
Dpph Radical Scavenging

Broccoli (Brassica oleracea var. italica) a cruciferous vegetable growing in Bangladesh was investigated to determine the fatty acid components, elemental concentrations, ascorbic acid content and antioxidant activity. A total of fifteen compounds were identified from the methylated esters of the fatty acids of flower and stem of broccoli when analyzed by GC-MS. The major compounds were linoleic acid, palmitic acid. linolenic acid, oleic acid, stearic acid and myrestic acid. Elemental analysis was done by XRF method which showed that both the flower and stem contained a significant amount of Na, K, Ca, Mg, Fe, P and Cl. However, heavy metals were not found in any of the broccoli samples. The broccoli flower and stem contained 42.20±0.263 mg and 120±0.254 mg of ascorbic acid per 100g of fresh samples. The methanol extract of broccoli exhibited the highest DPPH radical scavenging activity among methanol, acetone and water extracts. The indegenous broccoli was found to have highest DPPH radical scavenging activity (> 90%) which was comparable to standard ascorbic acid (98.22±1.122%) and BHA (96.01±0.983 %). Methanol extract of flower and stem exhibited highest ferrous ion chelating ability ((91.85±0.951 and 97.38±1.241) respectively whereas the ascorbic acid and BHA hardly demonstrated any ferrous ion chelating ability. These results clearly indicated that Bangladeshi broccoli has a significant potential for uses as nutrients and antioxidant suppliments. Key words: Broccoli; Fatty acids; Minerals; Vitamin C; Free radical scavenging power; Reducing power; Ferrous ion chelating ability. DOI: 10.3329/dujps.v9i1.7427 Dhaka Univ. J. Pharm. Sci. 9(1): 31-37 2010 (June)

Phytochemical Analysis, Antibacterial and Antioxidant Potential of Marine Red Seaweed Caulacanthusustulatus

2019 ◽  
Vol 12 (6) ◽  
pp. 4701-4708
Author(s):  
Mohideen Askar Nawas P ◽  
Sujatha Ravi
Keyword(s):  
Antibacterial Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Red Seaweed ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Hydroxyl Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Marine algae are known to produce a wide variety of bioactive secondary metabolites and several compounds have been derived from them for prospective development of novel drugs by the pharmaceutical industries. The marine red seaweed Caulacanthusustulatus, collected from the Kilakarai region located between (9.23135° N, 78.7844° E) Ramanathapuram District, Tamil Nadu, India. The seaweed extract was prepared from various solvent extracts namely aqueous, ethanol, methanol and acetone was tested for their phytochemical analysis, antibacterial activity against human pathogens viz., Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus and aquatic pathogens viz., Aeromonas hydrophila and Vibrio vulnificus using disc diffusion methods and in vitro antioxidant activity such as DPPH radical scavenging activity, ABTS radical scavenging activity and Hydroxyl radical scavenging activity of selected solvent extracts. Phytochemical analysis of sixteen different chemical compounds was carried out. The maximum nine phytochemical compounds were present in the methanol and ethanol extracts and the minimum six compounds were present in aqueous extract. The highest antibacterial activity was present in human pathogen P. aeruginosa (20.66 ± 1.1mm) likewise the aquatic pathogen the antibacterial activity was increase in Aeromonas hydrophila (20.33 ± 1.5mm). The methanol extract was significantly higher in DPPH radical scavenging activity (67.07 ± 0.5μg/ml), ABTS radical scavenging activity (72.4 ± 0.6 μg/ml) and Hydroxyl radical scavenging activity (54.06 ± 0.58μg/ml). This study indicates the potential use of red seaweed; in particularC.ustulatus extracts are treating human and aquatic bacterial pathogens and it could be a potential candidate for the natural compounds as antioxidant.

scholarly journals Structural Features of Sulfated Glucuronomannan Oligosaccharides and Their Antioxidant Activity

Marine Drugs ◽  
2018 ◽  
Vol 16 (9) ◽  
pp. 291 ◽  
Author(s):  
Weihua Jin ◽  
Langlang Ren ◽  
Bing Liu ◽  
Quanbin Zhang ◽  
Weihong Zhong
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Hydroxyl Radical Scavenging Activity ◽  
Dpph Radical Scavenging

Glucuronomannan oligosaccharides (Gs) were derived from fucoidan, which was extracted from the brown alga Sargassum thunbergii. Sulfated glucuronomannan oligosaccharides (SGs) were obtained by the sulfation of Gs. NMR techniques were used to reveal that the order of sulfation was Man-C6 > Man-C4 > Man-C1R > GlcA-C3 > Man-C3 > GlcA-C2. Finally, the antioxidant activities (hydroxyl radical scavenging activity, superoxide radical scavenging activity, reducing power and DPPH radical scavenging activity) of Gs and SGs were determined. The findings showed that the higher the degree of polymerization, the better the activity, except for the hydroxyl radical scavenging activity. In addition, the higher the sulfate content, the lower the activities for the reducing power and DPPH radical scavenging activity. Opposite results were found for the superoxide radical scavenging activity. Finally, compared with fucoidan, most Gs and SGs had higher antioxidant activity, suggesting that they might be good candidates for antioxidants.

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

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