scholarly journals Improving the Berry Quality and Antioxidant Potential of Flame Seedless Grapes by Foliar Application of Chitosan–Phenylalanine Nanocomposites (CS–Phe NCs)

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2287
Author(s):  
Gholamreza Gohari ◽  
Elnaz Zareei ◽  
Muhittin Kulak ◽  
Parisa Labib ◽  
Roghayeh Mahmoudi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Grape Berries ◽  
Pal Activity ◽  
Dpph Radical Scavenging

The production and sustainability of grape berries with high quality and health-promoting properties is a major goal. In this regard, nano-engineered materials are being used for improving the quality and marketability of berries. In this study, we investigated the potential role of chitosan–phenylalanine nanocomposites (CS–Phe NCs) in improving the quality of Flame Seedless (Vitis vinifera L.) grape berries, such as titratable acidity (TA), pH, total soluble solids (TSS), ascorbic acid, total phenolics, total flavonoids, anthocyanin, 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activity, and phenylalanine ammonia-lyase (PAL) activity. In this context, grape berries collected in two growing seasons (2018–2019) were screened. Regarding the experimental design, the treatments included chitosan at a 0.5% concentration (CS 0.5%), phenylalanine at 5 mM and 10 mM concentrations (Phe 5 mM and Phe 10 mM), and chitosan–phenylalanine nanocomposites (CS–Phe NCs) at 5 mM and 10 mM concentrations. The lowest TA was recorded in grape berries treated with CS–Phe NCs with a 10 mM concentration. However, treatments enhanced with TSS, which reached the highest value with 10 mM of CS–Phe NCs, were reflected as the highest ratio of TSS/TA with 10 mM of CS–Phe NC treatment. Nanocomposites (NCs) also increased pH values in both study years compared to the control. Similarly, the ascorbic acid and total phenolic content increased in response to NP treatment, reaching the highest value with 5 mM and 10 mM of CS–Phe NCs in 2018 and 2019, respectively. The highest flavonoid content was observed with 5 mM of CS–Phe NCs in both study years. In addition, the anthocyanin content increased with 5 and 10 mM of CS–Phe NCs. PAL activity was found to be the highest with 5 mM of CS–Phe NCs in both study years. In addition, in accordance with the increase in PAL activity, increased total phenolics and anthocyanin, and higher DPPH radical scavenging activity of the grapes were recorded with the treatments compared to the control. As deduced from the findings, the coating substantially influenced the metabolic pathway, and the subsequent alterations induced by the treatments were notably appreciated due to there being no adverse impacts perceived.

scholarly journals Chemical and Antioxidant Properties of Broccoli Growing in Bangladesh

1970 ◽  
Vol 9 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Ismet Ara Jahan ◽  
M Mostafa ◽  
Ishrat Nimmi ◽  
M Hemayet Hossain ◽  
Moinul Ahsan ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ferrous Ion ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Chelating Ability ◽  
Dpph Radical Scavenging

Broccoli (Brassica oleracea var. italica) a cruciferous vegetable growing in Bangladesh was investigated to determine the fatty acid components, elemental concentrations, ascorbic acid content and antioxidant activity. A total of fifteen compounds were identified from the methylated esters of the fatty acids of flower and stem of broccoli when analyzed by GC-MS. The major compounds were linoleic acid, palmitic acid. linolenic acid, oleic acid, stearic acid and myrestic acid. Elemental analysis was done by XRF method which showed that both the flower and stem contained a significant amount of Na, K, Ca, Mg, Fe, P and Cl. However, heavy metals were not found in any of the broccoli samples. The broccoli flower and stem contained 42.20±0.263 mg and 120±0.254 mg of ascorbic acid per 100g of fresh samples. The methanol extract of broccoli exhibited the highest DPPH radical scavenging activity among methanol, acetone and water extracts. The indegenous broccoli was found to have highest DPPH radical scavenging activity (> 90%) which was comparable to standard ascorbic acid (98.22±1.122%) and BHA (96.01±0.983 %). Methanol extract of flower and stem exhibited highest ferrous ion chelating ability ((91.85±0.951 and 97.38±1.241) respectively whereas the ascorbic acid and BHA hardly demonstrated any ferrous ion chelating ability. These results clearly indicated that Bangladeshi broccoli has a significant potential for uses as nutrients and antioxidant suppliments. Key words: Broccoli; Fatty acids; Minerals; Vitamin C; Free radical scavenging power; Reducing power; Ferrous ion chelating ability. DOI: 10.3329/dujps.v9i1.7427 Dhaka Univ. J. Pharm. Sci. 9(1): 31-37 2010 (June)

scholarly journals Antiglycation and Antioxidant Properties of Ficus deltoidea Varieties

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Nur Sumirah Mohd Dom ◽  
Nurshieren Yahaya ◽  
Zainah Adam ◽  
Nik Mohd Afizan Nik Abd. Rahman ◽  
Muhajir Hamid
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Carbonyl ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Ficus Deltoidea ◽  
Dpph Radical Scavenging

The present study aimed to evaluate the potential of standardized methanolic extracts from seven Ficus deltoidea varieties in inhibiting the formation of AGEs, protein oxidation, and their antioxidant effects. The antiglycation activity was analyzed based on the inhibition of AGEs, fructosamine, and thiol groups level followed by the inhibition of protein carbonyl formation. The antioxidant activity (DPPH radical scavenging activity and reducing power assay) and total phenolic contents were evaluated. After 28 days of induction, all varieties of Ficus deltoidea extracts significantly restrained the formation of fluorescence AGEs by 4.55–5.14 fold. The extracts also reduced the fructosamine levels by 47.0–86.5%, increased the thiol group levels by 64.3–83.7%, and inhibited the formation of protein carbonyl by 1.36–1.76 fold. DPPH radical scavenging activity showed an IC50 value of 66.81–288.04 μg/ml and reducing power activity depicted at 0.02–0.24 μg/ml. The extent of phenolic compounds present in the extracts ranged from 70.90 to 299.78 mg·GAE/g. Apart from that, correlation studies between the activities were observed. This study revealed that seven varieties of Ficus deltoidea have the potential to inhibit AGEs formation and possess antioxidant activity that might be attributed to the presence of phenolic compounds.

DPPH Radical Scavenging Activity of Kaempferol

2013 ◽  
Vol 781-784 ◽  
pp. 1294-1297 ◽  
Author(s):  
Ping Liu ◽  
Yun Tao Gao ◽  
Jiao Jiao Yu ◽  
Jia Wei Cha ◽  
Dong Mei Zhao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Absorption Spectrum ◽  
Reaction Time ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

The UV-Vis absorption spectrum of kaempferol and DPPH was investigated, the optimum determination wavelength and reaction time for determining the DPPH radical scavenging activity of kaempferol was 517 nm and 30 min, respectively. Kaempferol exhibited strong DPPH radical scavenging activity with a IC50 value of 0.004349 mg·mL-1, which is smaller than that of rutin, indicating that kaempferol has a stronger antioxidant activity than rutin.

scholarly journals IMPROVED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROSCOPY (HPLC/MS) METHOD FOR DETECTION OF ANTHRAQUINONES AND ANTIOXIDANT POTENTIAL DETERMINATION IN ALOE SINKATANA

2020 ◽  
Author(s):  
Asim Halfawi Shargi ◽  
Mohammed Aboied ◽  
Ibrahim ME ◽  
Fatehalrahman F Magbool
Keyword(s):  
Peer Review ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
E Mail ◽  
Dpph Radical Scavenging

Objectives:  Medicinal plants, either as an extract, pure compound or as a derivative, offer limitless opportunities for the discovery of new drugs. Sudan is a very rich source of medicinal plants which are used in the treatment of a wide range of diseases. Aloe sinkatana, has great potential to be developed as drug by pharmaceutical industries. The present study is undertaken to investigate the antioxidant potential of Aloe sinkatana by DPPH radical scavenging activity. In addition, the study also performed to explore the possibility of using HPLC-MS technique for the determination and analysis of Aloe sinkatana. Methods:  The extracts of Aloe sinkatana were analyzed for antioxidant activity by using DPPH free radical scavenging activity. The results indicated that the extracts showed a high effective free radical scavenging in the DPPH assay, also these extracts exhibited a noticeable antioxidant effect at low concentrations. Results:  During in vitro evaluation the antioxidant potential of methanolic extract was the highest, followed by aqueous extract  in DPPH radical scavenging activity. So the methanolic extract of the plant, exhibited a great antioxidant effect at 50 μg/ml which may be attributed to high phenolic content. Therefore, methanolic extract to be a more active radical scavenger than aqueous extract. The HPLC-MS analysis had shown the methanolic extract of Aloe sinkatana to be rich in the major anthraquinones and their glucosides, which revealed 9 compounds, and also UV spectroscopy detected the presence of two flavonoids. Conclusion:  The results indicated that the extracts of Aloe sinkatana  is a potential source of natural antioxidants or nutraceuticals with potential application to reduce oxidative stress with consequent health benefits. Due to stronger antioxidant potential and phytochemical composition, Aloe sinkatana could be proved as a valuable prospect in pharmaceutical formulations by taking part in the antioxidant defense system against generation of free radicals. Peer Review History: Received 26 March 2019; Revised 15 April; Accepted 4 May, Available online 15 May 2020 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file Average Peer review marks at initial stage: 4.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewer(s) detail: Name: Prof Cyprian Ogbonna ONYEJI Affiliation: Obafemi Awolowo University, Ile-Ife, Nigeria E-mail: [email protected]   Name: Dr. Gehan Fawzy Abdel Raoof Kandeel Affiliation: Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt E-mail: [email protected]   Name: Dr. Nyunaï Nyemb Affiliation: Ministry of Scientific Research and Innovation of Cameroon E-mail: [email protected]   Comments of reviewer(s): Similar Articles: ANTIHYPERGLYCEMIC AND ANTI-OXIDANT POTENTIAL OF ETHANOL EXTRACT OF VITEX THYRSIFLORA LEAVES ON DIABETIC RATS

scholarly journals Evaluation of antioxidant and antiinflammatory activity of ethanolic extracts of Polygonum senticosum in lipopolysaccharide-induced RAW 264.7 macrophages

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Chul Hwan Kim ◽  
Buyng Su Hwang ◽  
Yong Hwang ◽  
Young Taek Oh ◽  
Jin-Woo Jeong
Keyword(s):  
Antiinflammatory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Raw 264.7 ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Dpph Radical Scavenging Activity ◽  
Raw 264.7 Macrophages ◽  
Tnf Α ◽  
Dpph Radical Scavenging

Abstract Objectives This study aimed to examine the antioxidant activity and antiinflammatory effects of ethanol extract of Polygonum senticosum (EPS) on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Methods Antioxidant activity of EPS was assessed by radical-scavenging effects on ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. Proinflammatory markers produced by LPS-induced RAW 264.7 macrophages were quantified to assess the antiinflammatory activity of EPS. Results Our results showed that EPS significantly increased FRAP and DPPH radical-scavenging activity. Additionally, EPS reduced LPS-induced proinflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), along with proinflammatory cytokines, including tumor necrosis factor (TNF)-α and interleukin (IL)-1β, without significant cytotoxicity. EPS significantly downregulated the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), TNF-α, and IL-1β in LPS-stimulated RAW 264.7 macrophages. Positive correlations were noted between FRAP and DPPH radical-scavenging activity and antiinflammatory capacity. Conclusions Our results indicate that EPS downregulates the expression of pro-inflammatory mediators such as NO, PGE2, and cytokines (IL-1β and TNF-α) in LPS-stimulated RAW 264.7 macrophage cells. Further research is needed for its use as a treatment for inflammation and related diseases.

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