scholarly journals Determination of Various Antibiotic Residues in Milk Samples Sold in Ankara Markets by Thin Layer Chromotography and Bioautographic Method

2009 ◽  
Author(s):  
Füsun TEMAMOĞULLARI ◽  
Sezai KAYA
Keyword(s):  
Thin Layer ◽  
Antibiotic Residues ◽  
Milk Samples ◽  
Bioautographic Method

scholarly journals HPLC-DAD multi-residue method for determination of florfenicol, penicillin and tetracycline residues in raw cow milk

2021 ◽  
Vol 2 (3) ◽  
pp. 01-08
Author(s):  
Ali Jaber ◽  
Zeinab Zahreddine ◽  
Simon Abou Haidar ◽  
Chadi Hosri ◽  
Ghassan Ibrahim ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Raw Milk ◽  
Reversed Phase ◽  
Cow Milk ◽  
Antibiotic Residues ◽  
Milk Samples ◽  
Antibiotics Residues ◽  
Tetracycline Residues

The existence of antibiotic residues in edible products constitutes a health problem to the consumers. Reversed-phase high-performance liquid chromatography with diode array detection (HPLC–DAD) was optimized and validated for the simultaneous determination of florfenicol (FF), penicillin (PE), and tetracycline (TC) residues in dairy raw milk samples. The determination of these antibiotics was carried out on HP-ODS Hypersil C18 (5μm, 125*4 mm) column at a flow rate (1mL/min) and temperature (35 ⁰C). The extraction method includes deproteinization of the milk sample followed by a solid-phase extraction (SPE) clean-up. The method was validated according to the European Commission Decision 2002/657/EC and the International Conference of Harmonization Guidelines. The recoveries for the studied antibiotics ranged from 82–111.54 % making the method suitable for performing routine analysis. The proposed method was applied for the analysis of antibiotic residues in 50 dairy raw milk samples collected from many regions in Lebanon. The results showed the occurrence of these antibiotics residues in milk collected from different Lebanese regions. The numbers indicate that 22 % of milk samples were found to be positive for FF, 42 % for PE, and 28 % for TC residues.

scholarly journals Determination of antibiotics sensitivity profiles of bacteria isolated from raw milk

2016 ◽  
Vol 2 (3) ◽  
pp. 396-401
Author(s):  
Mukta Talukder ◽  
HM Manir Ahmed
Keyword(s):  
Food Contamination ◽  
Raw Milk ◽  
Animal Origin ◽  
Resistance Pattern ◽  
Antibiotic Residues ◽  
E Coli ◽  
Milk Samples ◽  
Resistant Strains ◽  
Antibiotics Sensitivity

The extensive progress of dairy sectors in a developing country like Bangladesh, led to widespread use of antibiotics to improve the health and productivity of animals. Prolonged usage may lead to antibiotic residues in foods of animal origin; hence, the emergence of antimicrobial resistant microorganisms. Accurate data on the antibiotic usage in livestock treatment, antibiotic residues and antimicrobial resistances in raw milk in Bangladesh are lacking. This study aimed to investigate the types and usages of antibiotics in cattle, their potential microbial resistances in raw milk samples. To do so, a total of 54 raw milk samples were evaluated and the bacterial isolates were identified and measured for resistance to 4 antibiotics most commonly used during bacterial infection Bangladesh. Amongst all 54 (100%) isolates were positive to S. aureus and 36 (66.67%) isolates were positive to E coli. Determination of the antibiotic resistance pattern of isolates showed that all isolates of S. aureus were resistant to cefoxitin (81.48%), ampicillin (64.81%), ciprofloxacin (51.85%), and gentamycin (70.37%). E. coli showed resistance to cefoxitin (69.44%), ampicillin (83.33%), ciprofloxacin (77.78%), and gentamycin (86.11%). However multidrug resistance pattern was also found. The obtained results provide evidence that antimicrobial resistant strains of the above pathogens have become remarkably widespread in raw milk. This requires better management for antibiotic usages among livestock farmers to control sources of food contamination and reduce the health risks associated with the development of resistant microbial strainsAsian J. Med. Biol. Res. September 2016, 2(3): 396-401

scholarly journals Validation of Thin-Layer Chromatography-Bioautographic Method for Determination of Streptomycin

2018 ◽  
Vol 4 (1) ◽  
pp. 34
Author(s):  
Isnaeni Isnaeni ◽  
Andri Astuti ◽  
Muhammad Yuwono
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Limit Of Detection ◽  
System Optimization ◽  
Detection Accuracy ◽  
Nutrient Agar Medium ◽  
Bioautographic Method ◽  
Layer Chromatography ◽  
Bio Assay

Background: A simple bio-assay for determination of streptomycin hyphenated with planar chromatography techniques was developed. Objective: This study aims to validate the method for identification and determination of streptomycin in injection preparations with TLC-bioautography. Methods: Thin Layer Chromatography (TLC) was performed on the silica Gel GF-254 using KH2PO4 solution as mobile solvent. The visualization was performed by spraying 2% resorcinol. Direct bi autography was developed using Escherichia coli ATCC 25922 as a bacterial test, grown on the nutrient agar medium at 37oC for 24 hours. The method was validated corresponding to linearity, limit of detection (LOD), intra day precision, and accuracy parameters. The accuracy was measured using streptomycin injection as a sample. Results: The Results showed that the KH2PO4 solution at 7.5% concentration was found to be the optimized solvent with Rf value of 0.5. The linear equation was y = 10.176x + 4.046 at 150 - 350 µg/mL concentration range with the linearity coefficient, Limit of Detection, accuracy, and variation coefficient were 0.9907; 40 ppm; 96.37 + 2.22% (with an RSD value of 2.31%); and 1.63 respectively. Conclusion: The prospective TLC-bioautographic method was applied for the identification and determination of streptomycin in a preparation using a single eluent KH2PO4. The eluent system optimization remains necessary for the identification and determination of the mixture of streptomycin with other antibiotics, such as aminoglycoside groups.

Thin Layer Chromatographic/Bioautographic Method for Identification of Antibiotic Residues in Animal Tissues

1987 ◽  
Vol 70 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Eli Neidert ◽  
Peter W Saschenbrecker ◽  
Frank Tittiger
Keyword(s):  
Thin Layer ◽  
Methanol Extract ◽  
Antibiotic Residues ◽  
The Matrix ◽  
Zones Of Inhibition ◽  
Tlc Plates ◽  
Solvent Systems ◽  
Bioautographic Method ◽  
Regulatory Analysis ◽  
Layer Chromatography

Abstract An analytical method for the identification of the residues from 14 commonly used antibiotics is presented. The technique is based on selective tissue extraction followed by thin layer chromatography (TLC)/bioautography. Antibiotic residues are extracted from the tissues with methanol and methanol-HCl (98 + 2). The methanol extract is further extracted with chloroform to isolate groups of antibiotics. The extracts are spotted onto TLC plates and developed in suitable solvent systems. Developed plates are placed on set medium seeded with Bacillus subtilis and a bioautograph is produced. The locations of zones of inhibition are used to identify antibiotic residues. Recoveries of antibiotics were quantitative, while the effect of naturally inhibiting components of the matrix was minimized. The sensitivity of the method can be adjusted through minor modifications, which allows its use in routine regulatory analysis.

scholarly journals Comparison of detection sensitivity of five microbial inhibition tests for the screening of aminoglycoside residues in fortified milk

2012 ◽  
Vol 30 (No. 4) ◽  
pp. 314-320 ◽  
Author(s):  
Z. Sýkorová Goffová ◽  
I. Kožárová ◽  
D. Máté ◽  
S. Marcinčák ◽  
Z. Gondová ◽  
...  
Keyword(s):  
Bacillus Stearothermophilus ◽  
Test Strain ◽  
Detection Sensitivity ◽  
Antibiotic Residues ◽  
Milk Samples ◽  
Microbial Inhibition ◽  
Fortified Milk ◽  
Maximum Residue Limits ◽  
Commission Regulation

The assessment of detection sensitivity of five microbial inhibition tests (MITs), STAR (screening test for antibiotic residues) with the test strain Bacillus subtilis BGA, Delvotest<sup>&reg; </sup>S&nbsp; P-NT, Total Antibiotics, Kalidos TB, and Kalidos MP with the test strain Bacillus stearothermophilus var. calidolactis to five aminoglycosides (AMGs), gentamicin, neomycin, streptomycin, kanamycin, and spectinomycin in fortified milk samples were studied. The sensitivity of MITs to AMGs was evaluated on the basis of experimental determination of detection limits (LODs) of MITs for AMGs. The LODs of these tests were compared with the maximum residue limits (MRLs) established for milk by the Commission Regulation (EU) No. 37/2010. LODs of STAR for AMGs in fortified milk samples were at the levels of MRL for neomycin (1.50 &micro;g/g), gentamicin (0.10&nbsp;&micro;g/g), streptomycin (0.20 &micro;g/g) and kanamycin (0.15 &micro;g/g). Spectinomycin (0.20 &micro;g/g) was not detected at the level of MRL. The LODs determined by Delvotest<sup>&reg; </sup>SP-NT, Total Antibiotics and Kalidos MP were comparable, but only gentamicin and neomycin were reliably detected at the levels of MRL. Kalidos TB was more sensitive to AMGs than Delvotest<sup>&reg; </sup>SP-NT, Total Antibiotics and Kalidos MP. Gentamicin, neomycin and streptomycin were detected at the levels of MRL. &nbsp;

Development of a method for multielemental determination in water by EDXRF with radioisotopic source of 238Pu.

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Camilo Fuentes Serrano ◽  
Juan Reinaldo Estevez Alvares ◽  
Alfredo Montero Alvarez ◽  
Ivan Pupo Gonzales ◽  
Zahily Herrero Fernandez ◽  
...  
Keyword(s):  
Thin Layer ◽  
Expanded Uncertainty ◽  
Considerable Decrease ◽  
X Ray ◽  
Precipitation Efficiency ◽  
Method Of Determination ◽  
Sensitivity Curves ◽  
Order Of Magnitude ◽  
Metrological Parameters

A method for determination of Cr, Fe, Co, Ni, Cu, Zn, Hg and Pb in waters by Energy Dispersive X Ray Fluorescence (EDXRF) was implemented, using a radioisotopic source of 238Pu. For previous concentration was employed a procedure including a coprecipitation step with ammonium pyrrolidinedithiocarbamate (APDC) as quelant agent, the separation of the phases by filtration, the measurement of filter by EDXRF and quantification by a thin layer absolute method. Sensitivity curves for K and L lines were obtained respectively. The sensitivity for most elements was greater by an order of magnitude in the case of measurement with a source of 238Pu instead of 109Cd, which means a considerable decrease in measurement times. The influence of the concentration in the precipitation efficiency was evaluated for each element. In all cases the recoveries are close to 100%, for this reason it can be affirmed that the method of determination of the studied elements is quantitative. Metrological parameters of the method such as trueness, precision, detection limit and uncertainty were calculated. A procedure to calculate the uncertainty of the method was elaborated; the most significant source of uncertainty for the thin layer EDXRF method is associated with the determination of instrumental sensitivities. The error associated with the determination, expressed as expanded uncertainty (in %), varied from 15.4% for low element concentrations (2.5-5 μg/L) to 5.4% for the higher concentration range (20-25 μg/L).

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