Intra-colony channel morphology in Escherichia coli biofilms is governed by nutrient availability and substrate stiffness

Nutrient-transporting channels are found throughout mature Escherichia coli biofilms, however the influence of environmental conditions on intra-colony channel formation is poorly understood. We report the effect of different substrate nutrient concentrations and agar stiffness on the structure and distribution of intra-colony channels in mature E. coli colony biofilms using fluorescence mesoscopy and quantitative image analysis. Intra-colony channel width was observed to increase non-linearly with radial distance from the centre of the biofilm and channels were, on average, 50% wider at the centre of carbon-limited biofilms compared to nitrogen-limited biofilms. Channel density also differed in colonies grown on rich and minimal medium substrates, with the former creating a network of tightly packed channels and the latter leading to well-separated, wider channels with easily identifiable edges. We conclude that intra-colony channel morphology in E. coli biofilms is influenced by both substrate composition and nutrient availability.

Channel morphology and prediction of mid-line channel migration in the reach of Ganga River using GIS and ARIMA modeling during 1975–2020

The dynamic nature of meandering poses several challenges in a river. The river Ganga shows severe bank erosion in many of its stretches which creates insecurity to the habitats. In the present study, channel morphology and lateral mid-line migration for 1975 to 2020 in 5 years intervals have been studied. The prediction of lateral mid-line migration from 2020 to 2050 by using multi-temporal Landsat satellite images was made by using the ARIMA model. The river reach was divided into 8 bends and 48 cross-sections were identified. The channel length was observed as 224.35 km in 1975 which reduces to 199.96 km in 2020. A decreasing trend was observed for the mean of channel length and meander ratio, and an increasing trend was noted in the mean of sinuosity ratio and tortuosity ratio. A total of 11 cross-sections showed the rightward shifting and 36 cross-sections showed the leftward shifting. Observed and predicted values showed a good R2 value of 0.90 and 0.89 at CS-24 and CS-25, respectively. The results may be used for planning and management of various river training work and understanding the river system dynamics.

Human impact on fluvial systems in Europe with special regard to today’s river restorations

Climate, geology, geomorphology, soil, vegetation, geomorphology, hydrology, and human impact affect river–floodplain systems, especially their sediment load and channel morphology. Since the beginning of the Holocene, human activity is present at different scales from the catchment to the channel and has had an increasing influence on fluvial systems. Today, many river–floodplain systems are transformed in course of river restorations to “natural” hydrodynamic and morphodynamic conditions without human impacts. Information is missing for the historical or rather “natural” as well as for the present-day situation. Changes of the “natural” sediment fluxes in the last centuries result in changes of the fluvial morphology. The success of river restorations depends on substantial knowledge about historical as well as present-day fluvial morphodynamics. Therefore, it is necessary to analyze the consequences of historical impacts on fluvial morphodynamics and additionally the future implications of present-day human impacts in course of river restorations. The objective of this review is to summarize catchment impacts and river channel impacts since the beginning of the Holocene in Europe on the fluvial morphodynamics, to critically investigate their consequences on the environment, and to evaluate the possibility to return to a “natural” morphological river state.