Questions about Johns Hopkins, his family, and their relationship to slavery have been raised by Johns Hopkins University, most directly in the announcement made by President Ronald Daniels on December 9, 2020, that, based on newly discovered records, the university’s founder owned five enslaved people who lived and worked at his properties in Baltimore in the mid-1800s. This announcement and other statements by the university have caused many people to doubt long-held beliefs about Johns Hopkins’ character and his support for anti-slavery causes. This paper investigates three of the primary assertions made in December 2020 by examining: (1) whether or not Johns Hopkins’ parents were slaveholders, (2) whether or not Johns Hopkins opposed slavery, and (3) whether or not census and other records prove that he owned enslaved people. After a careful review of the evidence uncovered to date, we argue that (a) Johns Hopkins’ parents and grandparents were devout Quakers who liberated the family’s enslaved laborers prior to 1800, (b) Johns Hopkins was an emancipationist who supported the movement to end slavery within the limits of the laws governing Maryland, and (c) the available documentation, including relevant tax records, does not support the university’s claim that Johns Hopkins was a slaveholder. We conclude with a discussion of the complex nature of Johns Hopkins’ economic relationship with the institution of slavery and a call for further research on his business networks, investment practices, and membership in the Religious Society of Friends.