Sintesis antigen AFB1-BSA dan konjugasi antibodi anti AFB1-BSA dengan nanopartikel emas sebagai pereaksi imunostrip

Aflatoksin B1 (AFB1) often contaminates a great variety of foods and animal feeds that will be dangerous if con-sumed by humans or animals. Rapid detection techniques that can be used in the field is really needed to monitor AFB1 contamination. The aims of this study were to perform synthesis of AFB1-BSA antigen and conjugation of antibody against AFB1-BSA to gold nanoparticle as immunostrip-test reagents. The AFB1-CMO was identified on TLC and AFB1-BSA was characterized using SDS PAGE. The AFB1-CMO formation indicated as a blue spot at 0.45 retention factor (Rf) on TLC and the AFB1-BSA antigen revealed as a single band protein at about 72 kDa molecular weight on the SDS PAGE. Conjugation of antibody against AFB1-BSA to gold nanoparticle resulted in the formation of red-dish purple compound which can be used for the detection of AFB1 on immunostrip. The optimum composition achieved in concentration of AFB1-BSA 1-1.5 mg/ml, IgG anti rabbit 0.1 mg/ml, and antibody against AFB1-BSA-gold nanoparticle conjugate in 0.5x0.5 cm² area characterized by the establishment of two reddish purple lines in the test and control zone.

Analysis and improvement of the “red/blue spot” of TFT-LCD

“Red/blue spot” is a common undesirable phenomenon in small and medium-sized TFT-LCD products, which directly affects the display quality of the product. It has always been a difficult problem in the display industry, which greatly reduces the market competitiveness of corresponding products. In this work, pressure tests are carried out on liquid crystal panels with different sizes and resolutions. The effects of different factors such as the flatness of the photo space, the distribution density of the spacers, and the glass thickness on the “red/blue spot” are compared. By increasing the flatness of the PS station, the septum distribution density, and the thickness of the glass, the sample’s anti-extrusion ability can be increased by 46.1%, 30%, and 23.1%, respectively. The experimental results can provide the basis for industry to further improve quality of products.

Jupiter's Interior as Revealed by Juno

Jupiter is in the class of planets that we call gas giants, not because they consist of gas but because they were primarily made from hydrogen-helium gas, which upon gravitational compression becomes a metallic fluid. Juno, in orbit about Jupiter since 2016, has changed our view: The gravity data are much improved, and the simplest interpretation of the higher order even harmonics implies that the planet may have a diluted central concentration of heavy elements. Jupiter has strong winds extending to perhaps ∼3,000-km depth that are evident in the odd zonal harmonics of the gravity field. Jupiter's distinctive magnetic field displays some limited local structure, most notably the Great Blue Spot (a region of downward flux near the equator), and some evidence for secular variation, possibly arising from the winds. However, Juno is ongoing; it has not answered all questions and has posed new ones. ▪ Juno's mission reveals Jupiter's interior. ▪ A core exists but is diluted by hydrogen. ▪ The mission revealed wind depth and magnetic field.

Blue, grey, and black colouration

Normal ethnic skin colouration is due to melanin in the skin. However, in this short chapter abnormal blue, grey, or black changes in skin colour, both localized or generalized, are discussed and illustrated by a few clinical images. The Mongolian blue spot that occurs as a physiological change in the skin of the majority of Asian, Black, and Chinese infants is due to melanin. It is uncommon in Caucasian skin types and not always recognized. A good illustration of the relatively common blue naevus is provided and much rarer examples discussed such as naevus of Ota, naevus of Ito, and alkaptonuria. In addition to melanin, other chromophores deposited in the skin may appear blue or black, such as haemoglobin or rarely drug deposits. The popularity of tattoos and associated problems are also mentioned.

Analysis and test of the central-blue-spot infall hallmark

Aims. The infall of material onto a protostar, in the case of optically thick line emission, produces an asymmetry in the blue- and red-wing line emissions. For an angularly resolved emission, this translates in a blue central spot in the first-order moment (intensity weighted velocity) map. Methods. An analytical expression for the first-order moment intensity as a function of the projected distance was derived, for the cases of infinite and finite infall radius. The effect of a finite angular resolution, which requires the numerical convolution with the beam, was also studied. Results. This method was applied to existing data of several star-forming regions, namely G31.41+0.31 HMC, B335, and LDN 1287, obtaining good fits to the first-order moment intensity maps, and deriving values of the central masses onto which the infall is taking place (G31.41+0.31 HMC: 70–120 M⊙; B335: 0.1 M⊙; Guitar Core of LDN 1287: 4.8 M⊙). The central-blue-spot infall hallmark appears to be a robust and reliable indicator of infall.

IDENTIFIKASI SENYAWA GOLONGAN FENOL DARI EKSTRAK ETANOL DAUN SIRIH HIJAU (PIPER BETLE LINN.) DENGAN METODE KLT-SPEKTROFOTODENSITOMETRI

Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, antifungi and antioxidant. The pharmacology activities of a herbal drug are influenced by the phytochemistry content, so in order to do a quality determination that provides phytochemistry profile and consistent pharmacology activities, a standardization is required. Fingerprint is the main standard to perform quality control for herbal drug. TLC-spectrophotodensitometry was used as the method in order to provide fingerprint profile of phenol compound. In this experiment, Piper betle leaves samples were extracted by reflux method using ethanol 96% as the solvent. Identification of phenol compound was done using TLC-spectrophotodensitometry with Silica gel 60 F254 as the stationary phase, toluena: ethyl acetate (93:7 v/v) as the mobile phase, FeCl3 and Folin-Ciocalteau as the reagent. The fingerprint profile of phenol compund was shown in Rf value 0,19; 0,42; and 0,62. Positive results of phenol compound are black spot on FeCl3 colour test and dark blue spot on Folin-Ciocalteau colour test. Maximum wavelength of phenol compound was 283 nm.