Atmospheric Methane Consumption and Methanotroph Communities in West Siberian Boreal Upland Forest Ecosystems

Upland forest ecosystems are recognized as net sinks for atmospheric methane (CH4), one of the most impactful greenhouse gases. Biological methane uptake in these ecosystems occurs due to the activity of aerobic methanotrophic bacteria. Russia hosts one-fifth of the global forest area, with the most extensive forest landscapes located in West Siberia. Here, we report seasonal CH4 flux measurements conducted in 2018 in three types of stands in West Siberian middle taiga–Siberian pine, Aspen, and mixed forests. High rates of methane uptake of up to −0.184 mg CH4 m−2 h−1 were measured by a static chamber method, with an estimated total growing season consumption of 4.5 ± 0.5 kg CH4 ha−1. Forest type had little to no effect on methane fluxes within each season. Soil methane oxidation rate ranged from 0 to 8.1 ng CH4 gDW−1 h−1 and was negatively related to water-filled pore space. The microbial soil communities were dominated by the Alpha- and Gammaproteobacteria, Acidobacteriota and Actinobacteriota. The major group of 16S rRNA gene reads from methanotrophs belonged to uncultivated Beijerinckiaceae bacteria. Molecular identification of methanotrophs based on retrieval of the pmoA gene confirmed that Upland Soil Cluster Alpha was the major bacterial group responsible for CH4 oxidation.

New record of Allamanda blanchetti Kunth (Apocynaceae) for the Brazilian Atlantic Forest

This study report the first record of Allamanda blanchetii Kunth in the Atlantic Forest, Brazil. This species was collected from an upland forest in Semi-deciduous Seasonal Forest. This occurrence adds new information about the distribution of this species and expands its range to Brazil, which is important for its conservation. We provide taxonomic information, distribution maps, conservation status assessment, photograps, and an identification key for the Allamanda L. species in the Atlantic Forest.

Isolation and Characterization of Biosurfactant-Producing Bacteria from Amapaense Amazon Soils

The objective of this research was to perform screening of biosurfactant-producing bacteria from Amapaense Amazon soils. Floodplain- and upland-forest soils of three municipalities of the Amapá state were isolated and identified. The isolates were cultured in nutrient broth with olive oil, and their extracts were evaluated according to drop collapse, oil dispersion, emulsification, and surface tension tests. From three hundred and eighteen isolates, the 43 bacteria were selected and identified by 16S rDNA gene sequencing, indicating the presence of three different genera, Serratia, Paenibacillus, and Citrobacter. The extracellular biosurfactant production pointed out the 15 most efficient bacteria that presented high emulsification capacity (E24 > 48%) and stability (less than 10% of drop after 72 h) and great potential to reduce the surface tension (varying from 49.40 to 34.50 mN·m−1). Cluster analysis classified genetically related isolates in different groups, which can be connected to differences in the amount or the sort of biosurfactants. Isolates from Serratia genus presented better emulsification capacity and produced a more significant surface tension drop, indicating a promising potential for biotechnological applications.

Can Understory Plant Composition and Richness Help Designate Riparian Management Zones in Mesic Headwater Forests of the Northeastern United States?

Riparian buffers implemented to minimize sediment, nutrients, and disturbance impacts on streams during forest operations vary greatly in the degree to which ecological criteria are used in their design. Because most forest operations are concentrated around headwater streams, our primary research objective was to identify a floristically based riparian boundary for headwater streams using plant species composition and indicator species to classify riparian environments distinct from the surrounding upland forest. Within three forested regions of the Northeast US, understory vegetation plots were sampled along perpendicular transects extending from the stream bank into the upland forest. At all sites, species richness was highest adjacent to the stream, decreasing exponentially within 6–12 m from the channel. Species composition closest to the stream was significantly different from all other lateral distances, but identified riparian indicator species were of limited practical use across all sites. However, changes in species richness can serve to identify a riparian area extent up to 6–12 m from headwater streams. Study Implications Riparian areas around headwater streams can be sensitive to forest management activities, particularly harvesting. Riparian management zone (RMZ) buffers around these streams vary in the degree to which they are based on ecological criteria; for example, fixed-width buffers may or may not adequately protect the riparian area. Our study within three forests of the Northeast detected a significant exponential decreasing trend in understory plant species richness within 6–12 m (20–40 ft) from the stream bank. We believe this ecologically based floristic zone closest to the stream represents the most sensitive part of the RMZ. This study recommends a 12 m (40 ft) zone to maintain the majority of the forest cover and minimize the impact of logging equipment. Foresters should be cognizant of this 12 m zone when implementing silvicultural activities and planning harvest access systems.

Description of five new species of Epitola sensu lato (Lepidoptera: Lycaenidae: Poritiinae) from West and Central Africa

Five new species of Epitola s.l. (Lycaenidae: Poritiinae) are described in the genera Stempfferia Jackson, 1962 and Cephetola Libert, 1999. Stempfferia salonga sp. n. was found in the ‘Cuvette Centrale’ of the Democratic Republic of Congo and a single specimen was identified also from southern Cameroon. The similar S. buea sp. n. is known only from the unique holotype collected at the foothills of Mount Cameroon, while S. michelliberti sp. n. is distributed in lowland rainforest between southeast Ivory Coast and Guinea. The new species of Cephetola were found only in Liberia. Cephetola praecox sp. n. is known from a single upland forest locality in the Putu Range, whereas C. wologizi sp. n. was collected also in upland forest in the Wologizi Mountains.

Mucor minutus (Baijal & B.S. Mehrotra) Schipper: a rare mucoralean fungi isolated for the first time in northeastern Brazil

Mucor minutus (Baijal & B.S. Mehrotra) Schipper is described for the first time from northeastern Brazil. The specimen was isolated from the soil of Florestas do Jussará, an upland forest fragment in Pernambuco state. In Brazil, M. minutus has previously been isolated only from the state of São Paulo from the soil of the Atlantic Forest. The occurrence of M. minutus in Florestas do Jussará is presented and discussed, and a detailed description and illustration of the specimen are presented. This article contributes to the knowledge of mucoralean distribution.

Spatial patterns of vegetation structure and structural diversity across edges between forested wetlands and upland forest in Atlantic Canada

Forested wetlands are an integral but understudied part of heterogeneous landscapes in Atlantic Canada, although they are known to provide habitat for species at risk. Our objectives were to explore patterns of forest structure across edges between forested wetland and upland forest, to locate changes in vegetation structure and to assess multivariate relationships in vegetation structure. Our study sites were in temperate (Acadian) forested wetland landscapes. We sampled trees and recorded canopy cover every 20 m along 120 m long transects. We estimated the cover of trees, saplings, shrubs in three height classes, Sphagnum, other bryophytes, lichens, graminoids, ferns and forbs in contiguous 1 x 1 m quadrats. We calculated structural diversity using the Shannon index and used wavelet analysis to assess spatial patterns. We found few clear patterns except for lower tree structural diversity at the edge of forested wetlands. Structural diversity was not a reliable measure for distinguishing forested wetland from upland forest. Forested wetlands are an integral part of many forested landscapes in Atlantic Canada but their detection and differentiation from surrounding ecosystem can be difficult. Policy should err on the side of caution when mapping forested wetlands and include them in wetland protection.