Baseline surface- and groundwater monitoring prior to an onshore shale gas operation in the Vale of Pickering, UK

A comprehensive programme of baseline groundwater hydrochemical monitoring has been carried out in connection with the proposed hydraulic fracturing of a 2 to 3 km deep Bowland Shale gas reservoir in borehole KM8 at Kirby Misperton, North Yorkshire, UK. The monitoring infrastructure encompassed: five on-site boreholes with hydraulically open intervals ranging from shallow weathered cover to a c. 200 m deep Corallian limestone aquifer, six off-site wells (hydraulically open in superficial materials and/or Kimmeridge Clay) and four surface water monitoring stations. Groundwater chemistry was high stratified with depth, ranging from slightly acidic, fresh, very hard Ca-HCO3-SO4 waters in shallow weathered cover, to brackish, calcium-depleted, highly alkaline waters in the Corallian aquifer. Dissolved methane was detected in most boreholes, with 10 µg/L being typical of shallow boreholes and around 50 mg/L in the Corallian. Low ethane concentrations and isotopic evidence suggest that the methane was predominantly microbial in origin (carboxylate fermentation at shallow depth, natural methanogenic CO2 reduction at greater depth). Elevated dissolved ethane (20-30 µg/L) was found in one well of intermediate depth, suggesting admixture of a possible thermogenic component, although this could be derived directly from the Kimmeridge Clay penetrated by the well.

Application of Clustering and Stepwise Discriminant Analysis Based on Hydrochemical Characteristics in Determining the Source of Mine Water Inrush

In order to explore the law of groundwater evolution, the water source connection between faults and aquifers and the main sources of mine water inrush in the deep mining area of Yangcheng Coal Mine in Jining City, 40 groups of hydrochemical samples were collected and analyzed by Piper Diagram and Durov Diagram. The results showed that the fluidity of groundwater developing to the deep became weaker, the value of total dissolved solids (TDS) became larger. So, the roof and floor of coal seam were more similar in water quality types due to the conduction of faults. Using principal component analysis (PCA) to the raw data, two principal components were extracted, and the principal component scores were used as clustering variables for hierarchical cluster analysis (HCA), 5 groups of abnormal water samples were eliminated and 3 clustering groups M1, M2 and M3 were obtained from the other water samples on the tree diagram. The results showed that the combination of HCA and hydrochemical analysis was more effective in screening water samples, and the 3 clustering groups could be qualified samples to represent 3 major aquifers (Taiyuan Formation limestone aquifer, Shanxi Formation sandstone aquifer and Ordovician limestone aquifer). Finally, taking M1, M2 and M3 as grouping variables, the discriminant functions f 1 , f 2 and f 3 of the 3 aquifers were obtained, the results of stepwise discrimination analysis (SDA) showed that the discrimination model established by using 25 groups of standard water samples could discriminate the known water samples with the correct rate of 96%, 10 groups of unknown water samples collected at the fault are identified as Taiyuan Formation limestone water samples, which was consistent with the classification results of HCA, proving that the water inrush of fault DF53 was from Taiyuan Formation limestone aquifer, while the fault had little influence on Ordovician limestone aquifer.

Nitrate removal by a novel lithoautotrophic nitrate-reducing iron(II)-oxidizing culture enriched from a pyrite-rich limestone aquifer

Nitrate removal in oligotrophic environments is often limited by the availability of suitable organic electron donors. Chemolithoautotrophic bacteria may play a key role in denitrification in aquifers depleted in organic carbon. Under anoxic and circumneutral pH conditions, iron(II) was hypothesized to serve as an electron donor for microbially mediated nitrate reduction by Fe(II)-oxidizing (NRFeOx) microorganisms. However, lithoautotrophic NRFeOx cultures have never been enriched from any aquifer and as such there are no model cultures available to study the physiology and geochemistry of this potentially environmentally relevant process. Using iron(II) as an electron donor, we enriched a lithoautotrophic NRFeOx culture from nitrate-containing groundwater of a pyrite-rich limestone aquifer. In the enriched NRFeOx culture that does not require additional organic co-substrates for growth, within 7-11 days 0.3-0.5 mM of nitrate was reduced and 1.3-2 mM of iron(II) was oxidized leading to a stoichiometric NO 3 - /Fe(II) ratio of 0.2, with N 2 and N 2 O identified as the main nitrate reduction products. Short-range ordered Fe(III) (oxyhydr)oxides were the product of iron(II) oxidation. Microorganisms were observed to be closely associated with formed minerals but only few cells were encrusted, suggesting that most of the bacteria were able to avoid mineral precipitation at their surface. Analysis of the microbial community by long-read 16S rRNA gene sequencing revealed that the culture is dominated by members of the Gallionellaceae family that are known as autotrophic, neutrophilic, microaerophilic iron(II)-oxidizers. In summary, our study suggests that NRFeOx mediated by lithoautotrophic bacteria can lead to nitrate removal in anthropogenically impacted aquifers. Importance Removal of nitrate by microbial denitrification in groundwater is often limited by low concentrations of organic carbon. In these carbon-poor ecosystems, nitrate-reducing bacteria that can use inorganic compounds such as Fe(II) (NRFeOx) as electron donors could play a major role in nitrate removal. However, no lithoautotrophic NRFeOx culture has been successfully isolated or enriched from this type of environment and as such there are no model cultures available to study the rate-limiting factors of this potentially important process. Here we present the physiology and microbial community composition of a novel lithoautotrophic NRFeOx culture enriched from a fractured aquifer in southern Germany. The culture is dominated by a putative Fe(II)-oxidizer affiliated with the Gallionellaceae family and performs nitrate reduction coupled to Fe(II) oxidation leading to N 2 O and N 2 formation without the addition of organic substrates. Our analyses demonstrate that lithoautotrophic NRFeOx can potentially lead to nitrate removal in nitrate-contaminated aquifers.

Constraining probabilistic chloride mass-balance recharge estimates using baseflow and remotely sensed evapotranspiration: the Cambrian Limestone Aquifer in northern Australia

Regional-scale estimates of groundwater recharge are inherently uncertain, but this uncertainty is rarely quantified. Quantifying this uncertainty provides an understanding of the limitations of the estimates, and being able to reduce the uncertainty makes the recharge estimates more useful for water resources management. This paper describes the development of a method to constrain the uncertainty in upscaled recharge estimates using a rejection sampling procedure for baseflow and remotely sensed evapotranspiration data to constrain the lower and upper end of the recharge distribution, respectively. The recharge estimates come from probabilistic chloride mass-balance estimates from 3,575 points upscaled using regression kriging with rainfall, soils and vegetation as covariates. The method is successfully demonstrated for the 570,000-km2 Cambrian Limestone Aquifer in northern Australia. The method developed here is able to reduce the uncertainty in the upscaled chloride mass-balance estimates of recharge by nearly a third using data that are readily available. The difference between the 5th and 95th percentiles of unconstrained recharge across the aquifer was 31 mm/yr (range 5–36 mm/yr) which was reduced to 22 mm/yr for the constrained case (9–31 mm/yr). The spatial distribution of recharge was dominated by the spatial distribution of rainfall but was comparatively reduced in areas with denser vegetation or finer textured soils. Recharge was highest in the north-west in the Daly River catchment with a catchment average of 101 (61–192) mm/yr and lowest in the south-east Georgina River catchment with 6 (4–12) mm/yr.

An Influence Study of Face Length Effect on Floor Stability under Water-Rock Coupling Action

The Taiyuan Formation limestone aquifer and Ordovician limestone aquifer are widely distributed in the coal seam floor of coal measures in North China; the water hazard safety problem of the stope floor under the influence of mining is very prominent. The risk of the water inrush from the coal seam floor is closely related to the degree of full exploitation, so it is necessary to study the stability of the stope floor under aquifer conditions, especially the influence of the working face length effect on floor stability. Through numerical simulation of water-rock coupling action, the mine pressure behaviors of the water-resisting floor under different face lengths were analyzed based on the measured formation permeability coefficient. The Fish program was used to adjust rocks entering the plastic failure state into a strain softening model to investigate the influence of the face length effect, the damage degree of the water-resisting floor, and the morphology and deformation bearing capacity of the failure zone. The results show the following: (1) the face length effect is one of the main influence factors of the failure mode and failure degree of surrounding rocks in the stope; (2) as the face length increases, the obvious pressure relief zone of surrounding rocks presents a staged change, and the obvious pressure relief zone at the seam roof and floor is in an obvious “reverse saddle shape”; (3) the closer to the seam floor, the more remarkable the rock softening characteristic because of the compaction action of gangues caving from the roof; and (4) the rock mass close to the seam floor undergoes local tensile failure, and the water-resisting floor near the coal wall at two sides mainly bears compaction-shear action, leading to compression-shear failure of the rock mass at the floor and formation of water-conducting fractures. The study results can provide a reference for taking precautionary measures of safety mining above a confined aquifer.