Study on the Effects of 8 Strains on Growth Promotion and Disease Resistance of Tomato (Solanum lycopersicum)

2013 ◽  
Vol 807-809 ◽  
pp. 1042-1045
Author(s):  
Xiao Deng ◽  
En Hui Cao ◽  
Chun Yuan Wu ◽  
Jing Kun Liu ◽  
Qin Fen Li
Keyword(s):  
Bacillus Subtilis ◽  
Disease Resistance ◽  
Leaf Area ◽  
Bacillus Licheniformis ◽  
Bacterial Wilt ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Chlorophyll Contents ◽  
Bacillus Laterosporus ◽  
Tomato Bacterial Wilt

The pot experiment was carried out to study the effects of eight functional strains on growth promotion of tomato and resistance to tomato bacterial wilt. The results indicated that the two strains both Paenibacillus polymyxa and Bacillus megaterium could significantly increase the plant height of tomato; the two strains of Paenibacillus azotoformans and Bacillus laterosporus could significantly improve the stem diameter of tomato; the three strains of Paenibacillus polymyxa, Bacillus laterosporus and Paenibacillus azotoformans could significantly increase the average leaf area and chlorophyll contents of Tomato; the four strains of Bacillus subtilis, Bacillus licheniformis, Paenibacillus polymyxa and Streptomyces microflavus could obviously inhibit occurrence of tomato bacterial wilt. All above results indicated that Paenibacillus polymyxa could not only significantly increase the height, the average leaf area and chlorophyll content of tomato plant, but also could obviously reduce the incidence of tomato bacterial wilt. So this strain can be used as one of core strains to construct multifunctional composite inoculants.

scholarly journals The impact of Paenibacillus polymyxa HY96-2 luxS on biofilm formation and control of tomato bacterial wilt

2019 ◽  
Vol 103 (23-24) ◽  
pp. 9643-9657 ◽  
Author(s):  
Jincui Yi ◽  
Daojing Zhang ◽  
Yuejuan Cheng ◽  
Jingjing Tan ◽  
Yuanchan Luo
Keyword(s):  
Bacterial Wilt ◽  
Late Stage ◽  
Biofilm Formation ◽  
Early Stage ◽  
Regulatory Gene ◽  
Paenibacillus Polymyxa ◽  
Deletion Strain ◽  
Biocontrol Efficacy ◽  
The Impact ◽  
Tomato Bacterial Wilt

Abstract The focus of this study was to investigate the effects of luxS, a key regulatory gene of the autoinducer-2 (AI-2) quorum sensing (QS) system, on the biofilm formation and biocontrol efficacy against Ralstonia solanacearum by Paenibacillus polymyxa HY96-2. luxS mutants were constructed and assayed for biofilm formation of the wild-type (WT) strain and luxS mutants of P. polymyxa HY96-2 in vitro and in vivo. The results showed that luxS positively regulated the biofilm formation of HY96-2. Greenhouse experiments of tomato bacterial wilt found that from the early stage to late stage postinoculation, the biocontrol efficacy of the luxS deletion strain was the lowest with 50.70 ± 1.39% in the late stage. However, the luxS overexpression strain had the highest biocontrol efficacy with 75.66 ± 1.94% in the late stage. The complementation of luxS could restore the biocontrol efficacy of the luxS deletion strain with 69.84 ± 1.09% in the late stage, which was higher than that of the WT strain with 65.94 ± 2.73%. Therefore, we deduced that luxS could promote the biofilm formation of P. polymyxa HY96-2 and further promoted its biocontrol efficacy against R. solanacearum.

scholarly journals Bacillus amyloliquefaciens Strain PMB05 Intensifies Plant Immune Responses to Confer Resistance Against Bacterial Wilt of Tomato

2020 ◽  
Vol 110 (12) ◽  
pp. 1877-1885
Author(s):  
Ting-Hsin Ho ◽  
Chiao-Yu Chuang ◽  
Jing-Lin Zheng ◽  
Hong-Hua Chen ◽  
Yu-Shen Liang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Bacterial Wilt ◽  
Bacillus Amyloliquefaciens ◽  
Plant Immunity ◽  
Reactive Oxygen Species Generation ◽  
Soft Rot ◽  
Limiting Factors ◽  
Callose Deposition ◽  
Bacillus Spp ◽  
Tomato Bacterial Wilt

Tomato is an economic crop worldwide. Many limiting factors reduce the production of tomato, with bacterial wilt caused by Ralstonia solanacearum being the most destructive disease. Our previous study showed that the disease resistance to bacterial soft rot is enhanced by Bacillus amyloliquefaciens strain PMB05. This enhanced resistance is associated with the intensification of pathogen-associated molecular patterns (PAMP)-triggered immunity (PTI). To determine whether the PTI-intensifying Bacillus spp. strains are able to confer disease resistance to bacterial wilt, their effects on PTI signals triggered by PAMP from R. solanacearum and on the occurrence of bacterial wilt were assayed. Before assay, a gene that encodes harpin from R. solanacearum, PopW, was applied as a PAMP. Results revealed that the B. amyloliquefaciens strain PMB05 was the one strain among 9 Bacillus rhizobacterial strains which could significantly intensify the PopW-induced hypersensitive response (HR) on Arabidopsis leaves. Moreover, we observed that the signals of PopW-induced reactive oxygen species generation and callose deposition were increased, confirming that the PTI was intensified by PMB05. The intensification of the PopW-triggered HR by PMB05 in Arabidopsis was reduced upon treatment with inhibitors in PTI pathways. Furthermore, the application of Bacillus spp. strains on tomato plants showed that only the use of PMB05 resulted in significantly increased resistance to bacterial wilt. Moreover, the PTI signals were also intensified in the tomato leaves. Taken together, we demonstrated that PMB05 is a PTI-intensifying bacterium that confers resistance to tomato bacterial wilt. Screening of plant immunity intensifying rhizobacteria is a possible strategy to control tomato bacterial wilt. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals MEKANISME ANTIBIOSIS BACILLUS SUBTILIS B315 UNTUK PENGENDALIAN PENYAKIT LAYU BAKTERI KENTANG

2015 ◽  
Vol 15 (1) ◽  
pp. 64 ◽  
Author(s):  
Nur Prihatiningsih ◽  
Triwidodo Arwiyanto ◽  
Bambang Hadisutrisno ◽  
Jaka Widada
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Leaf Area ◽  
Incubation Period ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Disease Index ◽  
Growth Promoter ◽  
Bacterial Wilt Disease

Antibiosis mechanism of Bacillus subtilis B315 for controlling potato bacterial wilt disease. Bacillus subtilis B315 isolated from rhizospheric potato has antibiosis mechanism against Ralstonia solanacearum in vitro and become potentially used as controlling method of bacterial wilt in the field. The objectives of this research were to study the mechanism of B.subtilis B315 in controlling bacterial wilt disease, to study of B. subtilis B315 potency as both biocontrol and plant growth promoter, and to evaluate the mechanism as biocontrol agent. This green house experiment used CRD (Completely Randomized Design) with 5 treatments and 6 replicates. The treatments were control (without B. subtilis B315), B. subtilis B315 wild type, antibiosis mutant M16, antibiosis mutant M4, and antibiosis mutant M14. Variables observed were incubation period, disease index, infection rate, effectiveness of control, and growth components (i.e number of bud, plant height, leaf area, plant fresh and dry weight). The result of this research showed that B. subtilis B315 could delay incubation period, suppressed the disease index up to 64,9% and could promote the plant growth (leaf area). B. subtilis B315 had the antibiosis and other mechanisms that induced sistemic resistance. The implication of this research was that B. subtilis B315 could be used for biocontrol the bacterial wilt and promoted the potato growth.

scholarly journals Isolation, Identification and Characterization of Rhizobacteria Strains for Biological Control of Bacterial Wilt (Ralstonia solanacearum) of Eggplant in China

Agriculture ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 37 ◽  
Author(s):  
Iman Sabah Abd Alamer ◽  
Ali Athafah Tomah ◽  
Bin Li ◽  
Jing-Ze Zhang
Keyword(s):  
Plant Growth ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Growth Promotion ◽  
Paenibacillus Polymyxa ◽  
Antagonistic Activity ◽  
Bacterial Strains ◽  
Seedling Length ◽  
Identification And Characterization

Bacterial wilt of eggplant is the most destructive disease caused by Ralstonia solanacearum throughout the world. Eleven bacterial strains with high antagonistic activity were obtained from 245 rhizobacteria. Based on analysis of morphology, 16S rRNA sequences, fatty acid profiles, gyrA and rpoB genes, they were identified as Pseudomonas putida (IMA3), Paenibacillus polymyxa (IMA5), Bacillus cereus (IMA4, IMA7 and IMA11) and the “operational group Bacillus amyloliquefaciens” (IMA1, IMA2, IMA6, IMA8, IMA9 and IMA10). The lipopeptide compounds produced by each strain also were determined. The biocontrol tests demonstrated that co-inoculation by strain IMA5 and the pathogen gave the greatest biocontrol efficiency of 87.0% and 69.2% 30 and 40 days after co-inoculation, respectively. Plant growth promotion tests revealed that IMA5 markedly promoted eggplant growth, enhancing aboveground seedling length and biomass by 60.8% and by 107.6% and underground root length and biomass by 33.0% and 69.2%, respectively. Hence, strain IMA5 could be considered for developing potential biocontrol agents and for promoting plant growth characteristics, to aid the management of the pathogen R. solanacearum in eggplants.

scholarly journals Effects of Biochar Amendment on Tomato Bacterial Wilt Resistance and Soil Microbial Amount and Activity

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Yang Lu ◽  
Shuang Rao ◽  
Fei Huang ◽  
Yixia Cai ◽  
Guoping Wang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Soil Bacteria ◽  
Soil Fungi ◽  
Peanut Shell ◽  
Soilborne Disease ◽  
Soil Microbial ◽  
Soil Microbial Population ◽  
Tomato Bacterial Wilt

Bacterial wilt is a serious soilborne disease of Solanaceae crops which is caused byRalstonia solanacearum. The important role of biochar in enhancing disease resistance in plants has been verified; however, the underlying mechanism remains not fully understood. In this study, two different biochars, made from peanut shell (BC1) and wheat straw (BC2), were added toRalstonia solanacearum-infected soil to explore the interrelation among biochar, tomato bacterial wilt, and soil microbial properties. The results showed that both BC1 and BC2 treatments significantly reduced the disease index of bacterial wilt by 28.6% and 65.7%, respectively. The populations ofR. solanacearumin soil were also significantly decreased by biochar application.Ralstonia solanacearuminfection significantly reduced the densities of soil bacteria and actinomycetes and increased the ratio of soil fungi/bacteria in the soil. By contrast, BC1 and BC2 addition to pathogen-infected soil significantly increased the densities of soil bacteria and actinomycetes but decreased the density of fungi and the ratios of soil fungi/bacteria and fungi/actinomycetes. Biochar treatments also increased soil neutral phosphatase and urease activity. Furthermore, higher metabolic capabilities of microorganisms by biochar application were found at 96 and 144 h in Biolog EcoPlates. These results suggest that both peanut and wheat biochar amendments were effective in inhibiting tomato bacterial wilt caused byR. solanacearum. The results suggest a relationship between the disease resistance of the plants and the changes in soil microbial population densities and activity.

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