Morphometric study of human placenta and an insight into its vascular pattern by corrosion cast technique

: The placenta is a chorioallantoic, deciduate, haemochorial and villous organ. It is a vital organ which transfers vital nutrients from the mother to the foetus and removes waste products from the foetus to the mother. There has been paucity of literature regarding the morphometric study of human placenta and its vascular pattern (magisterial or dispersed) by corrosion cast technique. Therefore, the present study was conducted on morphometry and vascular pattern of the human placentae.: To do the morphological and morphometric study of placenta and to study the vascular pattern of placenta by corrosion cast technique. Study was done on 43 placentae and its morphometric study and vascular pattern were studied by corrosion cast technique.: Oval was the most common shape of placenta observed in the present study. Out of 43 placentae, 22(51.16%) had oval, 16(37.20%) placentae had round shape followed by triangular in 3(6.97%) placentae. Only 1 (2.32%) placenta each found to be irregular and multilobed. Most common shape recorded in complicated pregnancy was oval followed by round. Magisterial pattern was found in 13.95% of placenta and was associated with oval shape constituting 25.58% of placentae. Mixed pattern was seen in 18.60% placentae and was associated with oval placentae(23.25%). The disperse pattern was seen in 4.65% and was associated with oval and irregular placentae each constituting(2.32%).: The vascular cast preparation of placentae in our study will further help in contribution towards the understanding of the placental vasculature. An effort should be made for performing more studies on placentae of complicated pregnancies eg: hypertension and diabetes etc.

Experimental Study on the Relationship between Tongue Temperature and the Lingual Circulatory System

To study the relationship between tongue temperature and the lingual circulatory system, the blood stasis and blood deficiency syndrome of traditional Chinese medicine (TCM) syndromes were researched by making animal model and vascular cast experiment. The influence of blood stasis and blood deficiency syndrome on tongue temperature and the lingual circulatory system were studied and analyzed. The experimental results indicated that the lingual circulatory system of blood stasis syndrome and that of blood deficiency syndrome were distinctly different in blood flow rate, vascular diameters and the density of small vessel. These differences in circulatory system could induce a change on lingual temperature. This result proves that the tongue temperature has relations with the change of lingual circulatory system.

Developmental study of chorioretinal vasculature demonstrated by vascular casts

Introduction: Methylmethacrylate is a very useful material for the preparation of vascular casts, and many reports have described the vascular of ocular tissues. We attempted to study the development of the rat ocular vasculature.Material and Methods: 20-day-old fetuses, and 1-day-old, 7-day-old, 14-day-old and 11-week-old rats were anesthetized, and the chest was opened by splitting the sternum. Warm Ringer solution containing heparin sodium was perfused through a catheter inserted into the left ventricle. Blood was removed through an opening in the right atrium. After 2% glutaraldehyde in 0.1M phosphate buffer had been perfused, a mixture of methylmethacrylate resin and catalyst was injected through the same catheter. The eyes were enucleated carefully and macerated in a bath of concentrated potassium hydroxide and sodium hypochlorite solution. The vascular cast was rinsed in distilled water and dried at room temperature or with t-butyl alcohol. After electroconductive staining with osmium tetroxide gas, the cast was mounted on stubs with carbon adhesive tape, and then spattercoated with gold for viewing in a scanning electron microscope.