AVALIAÇÃO DA DIVERSIDADE GENÉTICA DE CAPSICUM SPP. COM BASE EM DESCRITORES MORFOAGRONÔMICOS E BROMATOLÓGICOS

O gênero Capsicum pertence à família Solanaceae e possui grande diversidade evidenciada entre 38 espécies já catalogadas, todavia apenas cinco espécies são domesticadas, C. annuum, C. baccatum, C. pubescens, C. frutescens e C. Chinense. As pimentas possuem uma grande variabilidade fenotípica, principalmente em relação à coloração, forma e tamanho dos frutos, que pode ser analisada através de descritores morfoagronômicos, auxiliando nos estudos de diversidade genética da cultura. Objetivou-se com este estudo caracterizar com base em descritores morfoagronômicos e bromatológicos, 33 acessos de pimentas do banco de germoplasma do IFES Campus de Alegre e, com isso, estimar a divergência genética deles. Foram selecionados 22 descritores morfoagronômicas e 5 avaliações físico-químicas. Estimou-se a contribuição relativa das características através do método de Singh e a dissimilaridade genética entre os acessos empregando a distância de Mahalanobis e o método Unweighted Paired Group Methodusing Arithmetic Averages (UPGMA). O dendrograma de dissimilaridade foi composto por nove grupos e revelou que os acessos IFES58 e IFES78 são os que apresentam maior similaridade, enquanto os genótipos IFES58 e IFES96 foram os mais distantes geneticamente. As caracterizações morfoagronômica e físico-química foram eficientes para estimar a diversidade genética entre os 33 acessos de pimenta e destacar acessos com potencial para exploração em diferentes nichos de mercado para consumo in natura, processado ou para fins ornamentais. O estudo realizado evidencia a existência de alta variabilidade genética entre os acessos de Capsicum do banco de germoplasma do IFES Campus de Alegre.

Avaliação morfológica de Ananas comosus var. erectifolius (L. B. Smith) Coppens & F. Leal-Bromeliacea

O objetivo desse trabalho foi determinar descritores morfológicos de acessos de curauá através de caracteres morfológicos visando à avaliação dos germoplasmas, na Embrapa Amazônia Oriental, em Belém, PA. Foram estudados sete caracteres morfológicos quantitativos de 42 acessos de curauá, totalizando 210 indivíduos, conservados in vivo no Horto de Plantas Medicinais. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos caracteres avaliados nenhum se mostrou redundante, logo não houve descartes. Com base na distância Euclidiana média apresentaram três menores distâncias 0,052; 0,084 e 0,085 entre pares de acessos e permitiram separá-los em três grupos divergentes pelo método UPGMA e pelo método de Tocher houve a formação de quatro grupos divergentes. Os acessos de Ananas comosus var.erectifolius possuem variabilidade morfológica de acordo com os sete caracteres quantitativos em estudo, sendo os caracteres comprimento da folha, altura da planta e número de folhas os que mais contribuíram para a divergência entre os acessos.

Analysis of Burnout Levels of Judo Coaches in the COVID-19 Period: Mixed Method

This study was carried out to find out the burnout levels of judo coaches in COVID-19 period and to show how they will approach their profession and athletes in the period after the pandemic. 136 judo coaches from different coaching levels participated in the study. Maslach Burnout Inventory was used in the study. In addition, 3 qualitative questions were prepared by the researchers to find out the emotions of coaches during and after the pandemic and how they approached their athletes. IBM SPSS 22 statistical program was used to analyze the data in the study. Since the data were not normally distributed, non-parametric Mann Whitney U was used in paired group comparisons, while Kruskal Wallis H test was used for the comparison of more than two groups and significance level was taken as p < .05. Content analysis method was used to analyze the 3 qualitative questions. As a result, burnout levels of the judo coaches in the study were found to be low. No significant difference was found between the variables of gender, age and coaching level and burnout level. In addition, while coaches stated that they missed their profession and were waiting excitedly for trainings to start, more than half of them stated that their approaches to athletes will change after the pandemic.

Avaliação morfológica de Pilocarpus microphyllus Stapf Ex Wardleworth

Este trabalho objetivou avaliar acessos de jaborandi identificando os descritores morfológicos para a espécie. Foram avaliados 25 acessos, conservados in vivo na Embrapa Amazônia Oriental, em Belém, PA, totalizando 125 indivíduos, para oito caracteres morfológicos quantitativos. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos oito caracteres avaliados segundo os dois procedimentos utilizados nenhum se mostrou redundante, logo não houve descarte. As dissimilaridades encontradas com base na distância euclidiana - média padronizada apresentaram três menores distâncias 0,35; 0,44 e 0,46 entre pares de acessos e permitiram separá-los em três grupos divergentes pelos métodos UPGMA e pelo método de Tocher houve a formação de quatro grupos distintos. Dois caracteres apresentaram as maiores contribuições para a divergência, sendo eles largura e comprimento da folha, com 35,4%. Portanto, os acessos avaliados de jaborandi possuem variabilidade morfológica de acordo com os caracteres quantitativos: número de folíolos/folha, altura da planta; largura do folíolo, comprimento do pecíolo, comprimento da folha, largura da folha; comprimento do folíolo e comprimento da inflorescência, sendo os caracteres largura e comprimento da folha os que mais contribuíram para a divergência entre os acessos.

Avaliação morfológica de Carapichea ipecacuanha (Brot.) L. Andersson

O objetivo desse trabalho foi avaliar e determinar os descritores morfológicos de acessos de ipeca. Foram avaliados 66 acessos de ipeca conservados in vivo na Embrapa Amazônia Oriental, em Belém, PA, totalizando 330 indivíduos, para cinco caracteres morfológicos quantitativos. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos cinco caracteres avaliados segundo os dois procedimentos utilizados nenhum se mostrou redundante, logo não houve descartes. Com base na distância Euclidiana média apresentaram três menores distâncias 0,09; 0,17 e 0,20 entre pares de acessos e permitiram separá-los em três grupos divergentes pelo método UPGMA e pelo método de Tocher houve a formação de dois grupos divergentes. Três caracteres apresentaram as maiores contribuições para a divergência, a largura da folha, comprimento do pecíolo e altura da planta, com 80,04 %. Diante disso, todos os caracteres apresentaram contribuição para diferenciação entre acessos os acessos de C. Ipecacuanha, sendo largura da folha, comprimento do pecíolo e altura da planta os que respectivamente mais contribuíram para a divergência entre os acessos.

ENVIRONMENT OF ELECTROMAGNETIC COMPATIBILITY OF RADIO-ELECTRONIC COMMUNICATION MEANS

The conditions of ensuring electromagnetic compatibility of radio electronic means of mobile radio service have been analyzed. The stages of such analysis are outlined and a list of measures to be taken at each stage is given. The types of scenarios (paired, group) of interfering radio electronic means of mobile radio service are described. The technical specifications of the radiocommunication service equipment and antennas, which provide a statement of validation of the electromagnetic compatibility of the radiocommunication electronic means radiocommunication, are provided. An algorithm for determining the electromagnetic environment is proposed and recommendations for providing electromagnetic compatibility are offered.

Comparison of the c-MET gene amplification between primary tumor and metastatic lymph nodes in non-small cell lung cancer.

e23138 Background: Activation of c-MET lead to a wide range of biological activities. MET has recently been identified as a novel promising target in NSCLC, some c-MET inhibitors have been developed. The primary aim of this study was to evaluate the c-MET amplification status in advanced NSCLC and compare the consistency of c-MET amplification analyses in metastatic lymph nodes and tumor tissue.Methods: Real-time fluorescent quantitative polymerase chain reaction was used to test the tumor tissues in 368 patients of NSCLCs and 178 paired metastatic lymph nodes samples and compared the amplifications consistency inmetastatic lymph nodesand tissue samples, and analyzed the correlation between c-MET gene amplification and clinical characteristics of patients. Another 5 cases of normal lung tissue were taken as negative control.Results: The c-MET gene amplification rate was 8.97%(33/368) in tumor tissues . Of the178 paired cases, c-MET gene amplification was positive in 7.95%(15/178) cancerous tissuesand18.54%(33/178) in metastatic lymph nodes; Of these patients,13 were positive in the two kinds of samples. 20 cases in the paired group were detected positive c-MET in metastatic lymph nodes but was negative in cancerous tissues; 2 cases in the paired group were detected positive c-MET gene amplification in cancerous tissues but was negative in metastatic lymph nodes,there were statistically significant differences between the two samples (χ2= 45.536, P < 0.001). c-MET gene amplification was detected more in metastatic lymph nodes than the primary cancerous tissue. Consistency was evaluated by consistency test(Kappa = 0.482, P < 0.001).When lymph nodes were used as surrogate samples of primary cancerous tissues, sensitivity was86.67%, the specificity was 87.69%.Conclusions: More c-MET gene amplification positive were detected in metastatic lymph nodes compare with the primary cancerous tissue. c-MET gene amplification detected in lymph node metastases could screen more patients suitable for TKI therapy. Lymph node Metastasis can predict the c-MET gene amplification of primary tumor, and guide the clinical use of gene targeted drugs.

Patient versus clinician symptom reporting during chemoradiation for rectal cancer.

646 Background: Pelvic radiotherapy with concurrent 5-fluorouracil based chemotherapy (chemoradiation) is a component of standard therapy for patients with T3/T4 or node-positive rectal cancer. Chemoradiation can be associated with significant acute gastrointestinal toxicity. This study sought to retrospectively compare patient and clinician reports of acute symptoms experienced by rectal cancer patients receiving chemoradiation. Methods: The charts of 199 rectal cancer patients who received chemoradiation from 11/06 to 2/11 were reviewed. Clinicians assessed toxicity weekly using Common Terminology for Clinical Adverse Event (CTCAE) version 3.0. Patient-reported outcomes (PROs) were collected weekly, in clinic, beginning 9/09 using the 7-item Bowel Problems Scale. 197 patients had at least one clinician assessment or PRO and were eligible for this study. Patient and clinician assessments were compared among a subgroup of 65 patients (paired group) who had at least one patient and clinician assessment on the same date using descriptive statistics. Agreement between patient and clinician assessments was evaluated by Cohen’s kappa coefficient. Results: Characteristics were well-balanced between all rectal patients and the paired group, with the exception of the use of intensity modulated radiotherapy (IMRT). IMRT has been used increasingly over time, and IMRT was therefore used in a larger proportion of the paired group versus all patients (77% vs. 51%, respectively). Diarrhea and proctitis were reported more often by patients than clinicians throughout treatment. Uncorrected agreement for diarrhea and proctitis was 82% and 72%, respectively. Corrected for chance, Cohen’s kappa was .64 for diarrhea, indicating moderate agreement, and .22 for proctitis, indicating only slight agreement. Conclusions: Our findings suggest a discrepancy between clinician and patient symptom reports. Further study is warranted to discern potential benefits of including PROs in prospective studies, and to find whether PROs can help clinicians set patient expectations, and/or enhance communication for optimal symptom management.