scholarly journals Avaliação morfológica de Pilocarpus microphyllus Stapf Ex Wardleworth

2021 ◽  
Vol 10 (6) ◽  
pp. e23210615283
Author(s):  
Ruanny Karen Vidal Pantoja Portal Moreira ◽  
Osmar Alves Lameira ◽  
Meiciane Ferreira Campelo ◽  
Allan Cristiam Santos Ramires
Keyword(s):  
Group Method ◽  
Paired Group ◽  
Pilocarpus Microphyllus

Este trabalho objetivou avaliar acessos de jaborandi identificando os descritores morfológicos para a espécie. Foram avaliados 25 acessos, conservados in vivo na Embrapa Amazônia Oriental, em Belém, PA, totalizando 125 indivíduos, para oito caracteres morfológicos quantitativos. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos oito caracteres avaliados segundo os dois procedimentos utilizados nenhum se mostrou redundante, logo não houve descarte. As dissimilaridades encontradas com base na distância euclidiana - média padronizada apresentaram três menores distâncias 0,35; 0,44 e 0,46 entre pares de acessos e permitiram separá-los em três grupos divergentes pelos métodos UPGMA e pelo método de Tocher houve a formação de quatro grupos distintos. Dois caracteres apresentaram as maiores contribuições para a divergência, sendo eles largura e comprimento da folha, com 35,4%. Portanto, os acessos avaliados de jaborandi possuem variabilidade morfológica de acordo com os caracteres quantitativos: número de folíolos/folha, altura da planta; largura do folíolo, comprimento do pecíolo, comprimento da folha, largura da folha; comprimento do folíolo e comprimento da inflorescência, sendo os caracteres largura e comprimento da folha os que mais contribuíram para a divergência entre os acessos.

scholarly journals Avaliação morfológica de Ananas comosus var. erectifolius (L. B. Smith) Coppens & F. Leal-Bromeliacea

2021 ◽  
Vol 10 (9) ◽  
pp. e20010915284
Author(s):  
Meiciane Ferreira Campelo ◽  
Osmar Alves Lameira ◽  
Ruanny Karen Vidal Pantoja Portal Moreira ◽  
Allan Cristiam Santos Ramires
Keyword(s):  
Ananas Comosus ◽  
Group Method ◽  
Paired Group

O objetivo desse trabalho foi determinar descritores morfológicos de acessos de curauá através de caracteres morfológicos visando à avaliação dos germoplasmas, na Embrapa Amazônia Oriental, em Belém, PA. Foram estudados sete caracteres morfológicos quantitativos de 42 acessos de curauá, totalizando 210 indivíduos, conservados in vivo no Horto de Plantas Medicinais. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos caracteres avaliados nenhum se mostrou redundante, logo não houve descartes. Com base na distância Euclidiana média apresentaram três menores distâncias 0,052; 0,084 e 0,085 entre pares de acessos e permitiram separá-los em três grupos divergentes pelo método UPGMA e pelo método de Tocher houve a formação de quatro grupos divergentes. Os acessos de Ananas comosus var.erectifolius possuem variabilidade morfológica de acordo com os sete caracteres quantitativos em estudo, sendo os caracteres comprimento da folha, altura da planta e número de folhas os que mais contribuíram para a divergência entre os acessos.

scholarly journals Avaliação morfológica de Carapichea ipecacuanha (Brot.) L. Andersson

2021 ◽  
Vol 10 (6) ◽  
pp. e23110615282
Author(s):  
Meiciane Ferreira Campelo ◽  
Osmar Alves Lameira ◽  
Ruanny Karen Vidal Pantoja Portal Moreira ◽  
Allan Cristiam Santos Ramires
Keyword(s):  
Group Method ◽  
Paired Group

O objetivo desse trabalho foi avaliar e determinar os descritores morfológicos de acessos de ipeca. Foram avaliados 66 acessos de ipeca conservados in vivo na Embrapa Amazônia Oriental, em Belém, PA, totalizando 330 indivíduos, para cinco caracteres morfológicos quantitativos. Os dados foram submetidos as analises multivariadas, realizado a seleção direta e seleção com reanálise agrupadas em dois métodos e com base na distância Euclidiana média utilizada na formação dos agrupamentos pelos métodos Unweighted Paired Group Method Using ArithmeticAverages -UPGMA de otimização de Tocher e para análise de componentes principais. Dos cinco caracteres avaliados segundo os dois procedimentos utilizados nenhum se mostrou redundante, logo não houve descartes. Com base na distância Euclidiana média apresentaram três menores distâncias 0,09; 0,17 e 0,20 entre pares de acessos e permitiram separá-los em três grupos divergentes pelo método UPGMA e pelo método de Tocher houve a formação de dois grupos divergentes. Três caracteres apresentaram as maiores contribuições para a divergência, a largura da folha, comprimento do pecíolo e altura da planta, com 80,04 %. Diante disso, todos os caracteres apresentaram contribuição para diferenciação entre acessos os acessos de C. Ipecacuanha, sendo largura da folha, comprimento do pecíolo e altura da planta os que respectivamente mais contribuíram para a divergência entre os acessos.

Aplicabilidade dos óleos vegetais em tônicos capilar: uma revisão

2021 ◽  
Vol 10 (15) ◽  
pp. e124101522725
Author(s):  
Lorena Stefany Souza Silva ◽  
Jânio Sousa Santos
Keyword(s):  
Ricinus Communis ◽  
Rosmarinus Officinalis ◽  
Rosmarinus Officinalis L ◽  
Pilocarpus Microphyllus

O mercado cosmético tem investido no suprimento dos interesses do grupo populacional Wellness que busca um estilo de vida mais saudável e sustentável ao meio ambiente, levando a valorização dos ativos vegetais e sua incorporação aos produtos cosméticos e de higiene. Nesse sentido, o presente estudo tem como objetivo abordar a empregabilidade dos óleos de Pilocarpus microphyllus, Rícinus communis e Rosmarinus officinalis na composição de tônicos naturais para crescimento capilar. Para tal, realizou-se uma revisão bibliográfica qualitativa na qual adotou-se como critérios que incluam documentos de cunho cientifico que apresentou relação com a aplicabilidade de óleos vegetais em cosméticos para crescimento capilar, em especial as loções tônicas. Nesse contexto, os óleos vegetais destacam-se como importantes constituinte que agregam valor ao produto o qual é incorporado, promovendo ações terapêuticas menos abrasivas aos utentes, sustentabilidade e potencial econômico gerado a agricultura familiar. Os óleos vegetais de Pilocarpus microphyllus, Rícino communis e Rosmarinus officinalis L. demonstraram características em comum para o tratamento antimicrobiano, vasodilatador e desobstrutor dos folículos pilosos, podendo ser empregados para a incorporação em tônicos para crescimento capilar. Sugere-se novas pesquisas de cunho experimentais in vitro e in vivo acerca da incorporação dos óleos vegetais em formulações para crescimento capilar.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Structural analysis of the photosynthetic membrane from Ectothiorhodospira halochloris

1983 ◽  
Vol 41 ◽  
pp. 740-741
Author(s):  
H. Engelhardt ◽  
R. Guckenberger ◽  
W. Baumeister
Keyword(s):  
Lattice Structure ◽  
Bacterial Species ◽  
Hexagonal Lattice ◽  
Reaction Centre ◽  
Photosynthetic Membrane ◽  
Rhodopseudomonas Viridis ◽  
Photosynthetic Membranes ◽  
Ectothiorhodospira Halochloris ◽  
Main Components

Bacterial photosynthetic membranes contain, apart from lipids and electron transport components, reaction centre (RC) and light harvesting (LH) polypeptides as the main components. The RC-LH complexes in Rhodopseudomonas viridis membranes are known since quite seme time to form a hexagonal lattice structure in vivo; hence this membrane attracted the particular attention of electron microscopists. Contrary to previous claims in the literature we found, however, that 2-D periodically organized photosynthetic membranes are not a unique feature of Rhodopseudomonas viridis. At least five bacterial species, all bacteriophyll b - containing, possess membranes with the RC-LH complexes regularly arrayed. All these membranes appear to have a similar lattice structure and fine-morphology. The lattice spacings of the Ectothiorhodospira haloohloris, Ectothiorhodospira abdelmalekii and Rhodopseudomonas viridis membranes are close to 13 nm, those of Thiocapsa pfennigii and Rhodopseudomonas sulfoviridis are slightly smaller (∼12.5 nm).

The Infection of Cells by Bullet-Shaped Viruses

1969 ◽  
Vol 27 ◽  
pp. 372-373
Author(s):  
Frederick A. Murphy ◽  
Alyne K. Harrison ◽  
Sylvia G. Whitfield
Keyword(s):  
Electron Microscopy ◽  
Physical Properties ◽  
Host Cell ◽  
Thin Section ◽  
Cultured Cells ◽  
Cell Infection ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

Ultrastructure of melanocyte-keratinocyte interactions and pigment transfer in vitro

1978 ◽  
Vol 36 (2) ◽  
pp. 650-651
Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo
Keyword(s):  
Direct Injection ◽  
Skin Pigmentation ◽  
Freeze Fracture ◽  
Pigment Granule ◽  
Time Lapse ◽  
Ultrastructural Level ◽  
Lanthanum Tracer ◽  
A Cell

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.

Effect of Some Metabolic Inhibitors on Vinblastine-Induced Ribosomal-Granular Material Complexes

1971 ◽  
Vol 29 ◽  
pp. 548-549
Author(s):  
Awtar Krishan ◽  
Dora Hsu
Keyword(s):  
Granular Material ◽  
Rna Synthesis ◽  
Culture Medium ◽  
Actinomycin D ◽  
Metabolic Inhibitors ◽  
Protein And Rna Synthesis ◽  
Apparent Reduction ◽  
Plant Alkaloids ◽  
In Cells

Cells exposed to antitumor plant alkaloids, vinblastine and vincristine sulfate have large proteinacious crystals and complexes of ribosomes, helical polyribosomes and electron-dense granular material (ribosomal complexes) in their cytoplasm, Binding of H3-colchicine by the in vivo crystals shows that they contain microtubular proteins. Association of ribosomal complexes with the crystals suggests that these structures may be interrelated.In the present study cultured human leukemic lymphoblasts (CCRF-CEM), were incubated with protein and RNA-synthesis inhibitors, p. fluorophenylalanine, puromycin, cycloheximide or actinomycin-D before the addition of crystal-inducing doses of vinblastine to the culture medium. None of these compounds could completely prevent the formation of the ribosomal complexes or the crystals. However, in cells pre-incubated with puromycin, cycloheximide, or actinomycin-D, a reduction in the number and size of the ribosomal complexes was seen. Large helical polyribosomes were absent in the ribosomal complexes of cells treated with puromycin, while in cells exposed to cycloheximide, there was an apparent reduction in the number of ribosomes associated with the ribosomal complexes (Fig. 2).

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