Screening of Bacteria in the Biodegradation of Linear Alkyl Benzene Sulfonate (LBAS) From Car Wash Waste Water in Tehran (Iran)

Linear alkyl benzene sulfonate (LABS) is a substance used as a surfactant in anionic detergents. Today, it is the most widely used surfactant in detergent products after soap. The aim of this study was to screen effective isolates for the degradation of LABS from car wash effluents in Tehran (Iran). In this study, 10 effluent samples were randomly collected from car washes in Tehran (Iran). Isolation of mesophilic aerobic bacteria was performed by direct and pre-enrichment culture techniques. Biodegradation of sodium dodecyl sulfonate by isolates and turbidity was investigated by methylene blue active substance (MBAS) method, and the superior strain was identified by molecular method (Polymerase Chain Reaction, PCR), which approximately 86% of the material was degrade by the bacterial isolate, and the isolate was identified as Bacillus licheniformis.The results of MBAS method showed that the rate of LBAS degradation by bacteria was 86%, which indicates the high power of this bacterium to degrade LABS from sewage systems (car wash effluents). It was also shown that the concentration of LABS during 8 days of biodegradation period by this bacterium decreased from 80%, which indicates a significant decrease in LABS by Bacillus licheniformis. According to our results, Bacillus licheniformis can be used to dagrade LABS and its degradation in wastewater treatment systems of car wash effluents whose effluents are based on alkyl benzene sulfonates.

Primary, secondary screening and evaluation of LAS degrading potential of soil bacteria isolated from Vidya Pratishthan’s college area

Inefficiency of use of soap in hard water popularized the use of anionic surfactants namely, branched alkylbenzene sulphonates (BAS) and Linear alkyl benzene sulphonates (LAS) in detergents. The low cost and superior solubility in hard water had made them popular and first choice of many household care-products. However, the disadvantage associated with their use was formation of stable foam over waterbodies which is detrimental for both the aquatic plants and animals. The current study involves primary and secondary screening of LAS degrading organisms and evaluates their potential. The three isolates screened isolate 1 can degrade LAS to the tune of 66% in 5 days, isolate 2 and isolate 3 degrade 72.16% and 68.25% respectively in 4 days.

PENGGUNAAN KARBON AKTIF DAN ZEOLIT TAK TERAKTIVASI DALAM ALAT PENYARING AIR LIMBAH LAUNDRY

Peningkatan jumlah jasa laundry sebanding dengan limbah yang dihasilkan yang biasanya dibuang langsung ke lingkungan tanpa adanya pengolahan. Limbah laundry mengandung surfaktan dalam jumlah besar, yaitu jenis surfaktan anionik Alkyl Benzene Sulfonates (ABS) dan Linear Alkyl Benzene Sulfonates (LAS), yang merupakan senyawa kimia yang berpotensi bahaya bagi kesehatan dan lingkungan sehingga perlu upaya untuk pengolahan limbah tersebut. Penelitian ini bertujuan mengetahui efisiensi alat penyaring limbah laundry dengan prinsip absorbsi dan filtrasi. Penelitian dilakukan dengan metode eksperimen dengan 3 perlakuan pada alat penyaring yaitu menggunakan (1) karbon aktif, (2) zeolit dan (3) kombinasi karbon aktif-zeolit. Hasil penelitian menunjukkan bahwa penggunaan alat penyaring dengan karbon tak teraktivasi dapat meningkatkan Total Dissolved Solid (TDS) dan kadar surfaktan, sedangkan alat penyaring dengan zeolit tak teraktivasi dapat menurunkan TDS namun meningkatkan kadar surfaktan sampai 8 kali lipat. Hasil penelitian mengungkapkan fakta bahwa penggunaan karbon aktif dan zeolit tak teraktivasi justru berpotensi mencemari lingkungan. Sehingga untuk tujuan pengendalian pencemaran lingkungan, perlu dilakukan aktivasi terlebih dahulu terhadap karbon aktif dan zeolit sebelum diaplikasikan pada alat penyaring. Kata kunci: alat penyaring, karbon aktif, limbah laundry, surfaktan, zeolite. The increasing number of laundry services is directly proportional to the amount of waste produced which is usually discharged directly into the environment without any processing. Laundry wastes contain large amounts of surfactants, namely anionic surfactants Alkyl Benzene Sulfonates (ABS) and Linear Alkyl Benzene Sulfonates (LAS), which are chemical compounds that are potentially dangerous to health and the environment so efforts are needed to treat these wastes. This study aims to determine the efficiency of laundry waste filter equipment with the principle of absorption and filtration. The study was conducted with an experimental method with 3 treatments on filter devices using (1) activated carbon, (2) zeolite and (3) combination of activated carbon-zeolite. The results showed that the use of a filter device with activated carbon can increase Total Dissolved Solid (TDS) and surfactant levels, while the filtering device with zeolite not activated can reduce TDS but increase surfactant levels up to 8 times. The results of the study revealed the fact that the use of activated carbon and zeolites not activated actually has the potential to pollute the environment. So for the purpose of controlling environmental pollution, it is necessary to first activate the activated carbon and zeolite before it is applied to the filter.Keywords: activated carbon, filtering, laundry waste, surfactant, zeolite.

ANALISIS KEMAMPUAN TANAH DI DAERAH PEMUKIMAN KOTA BENGKULU DALAM MENJERAP ALKYL BENZENE SULPHONATE (ABS) DAN LINEAR ALKYLBENZENE SULPHONATE (LAS)

This research aims to know the ability of land in a residential area of the city of Bengkulu in adsorbed Alkyl Benzene Sulphonate (ABS) and Linear Alkyl Benzene Sulphonate (LAS). Soil samples were taken at five-point i.e. the backyard of Balai Buntar, Fatmawati Airport, Beringin Raya , Pematang Gubernur and Simpang Nakau with a drill ground at a depth of 20 cm and cleaned from garbage impurity. Then the soil characterized include moisture content, pH, texture and the organic content and ABS and ALS adsorbsition measured using UV-vis spectrophotometers att 222-223 nm wavelengths. In this research tested the influence of pH, pulp and optimum contact time contacts from soil sample at in ABS and ALS adsorbsition The adsorbtion pattern is known as the Freundlich and Langmuir parameters. Based on research, generally, it can be concluded that the land in the residential area of Bengkulu city has characteristic average water content – average 23%, pH average – average 5.35 and it's Turn dusty clay. The optimum condition from soil in adsorbs from Alkyl Benzene Sulphonate (ABS) occurs at pH 3, a mass of 1 g and 2 hours of contact time with the adsorption capacity of 0.874 mg/g, while for Linear Alkyl Benzene Sulphonate (LAS) adsorption the optimum soil conditions occur at pH 2, mass 0.5 g and 1-hour contact time with the adsorption capacity of 1.607 mg/g. The pattern of soil adsorption against ABS tends to follow the Langmuir equation whereas against LAS tend to follow the Freundlich equation. Adsorpstion of soil samples in Bengkulu City against ABS belongs to low and adsorpstion against LAS belongs to very low