Dehydrogenase polymorphism was studied in 36 sour cherry (Prunus cerasus L.),
sweet cherry (Prunus avuim L.), mahaleb (Prunus mahaleb L.), ground cherry
(Prunus fruticosa Pall.), duke cherry (Prunus gondounii Redh.), Japanese
flowering cherry (Prunus serrulata Lindl.) and four iterspecific hybrids
(standard cherry rootstocks ?Gisela 5?, ?Gisela 6?, ?Max Ma? and ?Colt?).
Inner bark of one-year-old shoots, in dormant stage, was used for enzyme
extraction. Vertical PAGE was used for isoenzyme analysis: alcohol
dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase
(GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH),
phosphogluconate dehydrogenase (PGD), and shikimate dehydrogenase (SDH). All
studied systems were polymorphic at 10 loci: Adh -1 (3 genotypes) and Adh-2
(5 genotypes), Fdh-1 (2 genotypes), Gdh-1 (3 genotypes), Idh-1 (4 genotypes)
i Idh -2 (5 genotypes), Mdh-1 (3 genotypes), Pgd-1 (4 genotypes), Sdh-1 (1
genotype) i Sdh-2 (3 genotypes). Cluster analysis was used to construct
dendrogram on which four groups of similar genotypes were separated. Obtained
results indicate that studied enzyme systems can be used for determination of
genus Prunus, subgenus Cerasus. Among studied enzyme systems ADH, IDH and SDH
were the most polymorphic and most useful to identify genetic variability.
Polymorphism of FDH and GDH in genus Prunus, subgenus Cerasus was described
first time in this work. First results for dehydrogenase variability of
Oblacinska indicate that polymorphism of loci Idh-2 and Sdh-2 can be useful
for discrimination of different clones.