scholarly journals Dehydrogenase isoenzyme polymorphism in genus Prunus, subgenus Cerasus

Genetika ◽  
2012 ◽  
Vol 44 (3) ◽  
pp. 619-632 ◽  
Author(s):  
Slavica Colic ◽  
Vera Rakonjac ◽  
Milica Fotiric-Aksic ◽  
Dragan Nikolic ◽  
Vladislav Ognjanov ◽  
...  

Dehydrogenase polymorphism was studied in 36 sour cherry (Prunus cerasus L.), sweet cherry (Prunus avuim L.), mahaleb (Prunus mahaleb L.), ground cherry (Prunus fruticosa Pall.), duke cherry (Prunus gondounii Redh.), Japanese flowering cherry (Prunus serrulata Lindl.) and four iterspecific hybrids (standard cherry rootstocks ?Gisela 5?, ?Gisela 6?, ?Max Ma? and ?Colt?). Inner bark of one-year-old shoots, in dormant stage, was used for enzyme extraction. Vertical PAGE was used for isoenzyme analysis: alcohol dehydrogenase (ADH), formate dehydrogenase (FDH), glutamate dehydrogenase (GDH), isocitrate dehydrogenaze (IDH), malate dehydrogenase (MDH), phosphogluconate dehydrogenase (PGD), and shikimate dehydrogenase (SDH). All studied systems were polymorphic at 10 loci: Adh -1 (3 genotypes) and Adh-2 (5 genotypes), Fdh-1 (2 genotypes), Gdh-1 (3 genotypes), Idh-1 (4 genotypes) i Idh -2 (5 genotypes), Mdh-1 (3 genotypes), Pgd-1 (4 genotypes), Sdh-1 (1 genotype) i Sdh-2 (3 genotypes). Cluster analysis was used to construct dendrogram on which four groups of similar genotypes were separated. Obtained results indicate that studied enzyme systems can be used for determination of genus Prunus, subgenus Cerasus. Among studied enzyme systems ADH, IDH and SDH were the most polymorphic and most useful to identify genetic variability. Polymorphism of FDH and GDH in genus Prunus, subgenus Cerasus was described first time in this work. First results for dehydrogenase variability of Oblacinska indicate that polymorphism of loci Idh-2 and Sdh-2 can be useful for discrimination of different clones.

2012 ◽  
Vol 64 (2) ◽  
pp. 63-68 ◽  
Author(s):  
Stanisław Wociór ◽  
Irena Wójcik ◽  
Salwina Palonka

The present study, carried out in the period 2007-2009 in the Sandomierz Upland, did not show a significant effect of foliar fertilization on trunk thickness and canopy volume in the cultivar Łutówka. However, under the influence of urea applied three times in combination with Florovit at a concentration of 0.5%, a clear increasing trend was observed in the above mentioned parameters used to assess the strength of growth. In years of abundant fruiting, this study showed a significant increase, or on the verge of significance, in yield after threefold application of urea and Florovit in combination. A small insignificant effect of fertilization on fruit weight shows that the growth in yield after the application of fertilization was produced as a consequential effect of a significant increase in the length of one-year shoots and thereby in flowering intensity of the 'Łutówka' cherry trees. In the case of the sour cherry cultivar Łutówka bearing fruit on one-year shoots, foliar feeding after flowering using multi-nutrient fertilizers and urea can be recommended as a technological treatment, in particular in years of abundant fruiting.


2017 ◽  
Author(s):  
Mikhail Yu. Kulikov ◽  
Anton A. Nechaev ◽  
Mikhail V. Belikovich ◽  
Tatiana S. Ermakova ◽  
Alexander M. Feigin

Abstract. The Technical Note presents a statistically correct approach to evaluating simultaneous measurements of several atmospheric components under the assumption of photochemical equilibrium. We consider simultaneous measurements of OH, HO2, and O3 at the altitudes of the mesosphere as a specific example and their daytime photochemical equilibrium as an evaluating relationship. A simplified algebraic equation relating local concentrations of these components in the 50–100 km altitude range has been derived. The parameters of the equation are air temperature, air concentration, local zenith angle, and the rates of 9 reactions. We have performed one-year simulation of the mesosphere and lower thermosphere using a 3D chemical-transport model. The simulation shows that the discrepancy between the calculated evolution of the components and the equilibrium value given by the equation does not exceed 3–4 % in the full range of altitudes independent of season or latitude. We have developed the technique of statistic Bayesian evaluation of simultaneous measurements of OH, HO2 and O3 based on the equilibrium equation taking into account the measurement error. The first results of application of the technique to MLS/Aura data are presented in this Technical Note. It has been found that the satellite data of HO2 distribution regularly demonstrates essentially lower altitudes of mesospheric maximum of this component. This has also been confirmed by offline retrieval of HO2 from the MLS primary data.


2014 ◽  
Vol 42 (2) ◽  
pp. 488-494 ◽  
Author(s):  
DuÅ¡ica DORIC ◽  
Vladislav OGNJANOV ◽  
Mirjana LJUBOJEVIC ◽  
Goran BARAC ◽  
Jovana DULIC ◽  
...  

The paper presents a protocol for micropropagation of Prunus sp. rootstocks included in the sweet and sour cherry breeding program. Germplasm diversity for rootstock breeding derives from natural populations, where conditions and biological vectors for systematic infection with viral diseases are constantly present. The establishment of aseptic culture depends primarily on the explant type, as all selections were collected from natural habitat. For nearly all investigated selections, dormant buds were the favored source, due to enabling rosette initiation in more than 58% cases. In P. cerasus L. selections, 100% contamination was noted when shoot tips were used as an explant source. Significant influence of the double-phase medium on the number and height of multiplied shoots was observed in the standard cherry rootstock, ‘Gisela 6’. For P. fruticosa Pall., selection ‘SV1’ and ‘SV2’, and P. cerasus ‘D6’ selection, the double-phase medium also had a significant effect on the height of multiplied shoots, when compared to solid DKW (Driver and Kuniyuki Walnut) medium. Genetic variability of selections within the investigated species resulted in variable plant rooting success. Adding Fe-EDDHA (Ethylenediamine di-2-hydroxy-phenyl acetate ferric) in the 200 mg l-1 concentration to the rooting medium significantly enhanced the percentage of rooted plants. The highest rooting percentage was noted for ‘Gisela 6’ and ‘D6’ genotype at 1 mgl-1 IBA (indole-3-butyric acid), while 0.8 mgl-1 was the optimum concentration for P. mahaleb L. ‘M1’ selection. P. fruticosa genotypes required significantly higher IBA concentration for rooting (2.5 and 3.5 mg l-1).


2000 ◽  
Vol 197 ◽  
pp. 161-174
Author(s):  
Gary J. Melnick

The Submillimeter Wave Astronomy Satellite (SWAS) was successfully launched on 5 December 1998 with the goals of studying: (1) the distribution of oxygen in the interstellar medium; (2) the role of H2O and O2 as gas coolants; and (3) the UV-illuminated surfaces of molecular clouds. To achieve these goals, SWAS is conducting pointed observations of dense (n(H2) > 103 cm–3) molecular clouds throughout our Galaxy in either the ground-state or a low-lying transition of five astrophysically important species: H2O, H218O, O2, CI, and 13CO. SWAS has made great strides in each of these areas of investigation. This paper will summarize our H2O and O2 findings one year into the mission.


2011 ◽  
Vol 31 (No. 2) ◽  
pp. 47-57 ◽  
Author(s):  
J. Blažková ◽  
I. Hlušičková

Ten clonal dwarf or semi-dwarf sweet cherry rootstocks were evaluated for 5 growing seasons in a orchard that was established in the spring 1999 at Holovousy within international cherry rootstock trials that are co-ordinated in Europe by the Danish Institute of Plant and Soil Science at Aarslev, Denmark. Lapins cv. was used as a scion tester for all these rootstocks; five of them were also tested by Regina cv. Among the new rootstocks, G 195/5 was evaluated as the most promising for Lapins cv. It produced trees even smaller than P-HL-A or Tabel (Edabriz), with higher yield efficiency and good fruit size. In agreement with the results of earlier experiments, both P-HL-A and Tabel (Edabriz) proved to be quite reliable rootstocks for modern sweet cherry orchards established there. Among the semi-dwarf rootstocks, G 154/7 also seemed to be interesting because of high yields and fruit size. Weiroot 158 was found as relatively the most promising rootstock for Regina cv. On the other hand, the most disappointing results were obtained with the rootstock Weiroot 53 because of its inferior graft compatibility and its negative influence on fruit size. Some of the tested rootstocks significantly changed the time of flowering and the time of fruit ripening. Several relationships between the observed characteristics were found within this study and they are more thoroughly discussed.  


Bone ◽  
2011 ◽  
Vol 48 (1) ◽  
pp. S24 ◽  
Author(s):  
S. Singh* ◽  
J. Cuzick ◽  
G.M. Blake ◽  
D. Mesher ◽  
R. Patel ◽  
...  

1962 ◽  
Vol 40 (8) ◽  
pp. 1041-1049 ◽  
Author(s):  
R. S. Willison

One-year-old virus-free sour cherry trees of the variety Montmorency were inoculated singly with buds from different source trees infected with sour cherry yellows, necrotic ring spot, prune dwarf, and tatter leaf respectively. The infections thus established in the test trees were later challenged by inoculation with isolates from different sources. Conspicuous recurrent symptoms occurred when infection from a tatter leaf isolate was challenged by either of two necrotic ring spot virus strains, but not when the order of challenge was reversed. These symptoms are interpreted as a synergistic response to infection by two viruses, one from each source, within a limited range of interaction. Trees previously infected with necrotic ring spot when reinoculated with cherry yellows isolates showed symptoms of cherry yellows, usually preceded by secondary shock. The reciprocal challenges, on the other hand, induced no symptoms of necrotic ring spot, probably because each of the cherry yellows sources carried a strain of the necrotic ring spot virus. There were indications, however, of interference between the two viruses. Infection with the prune dwarf virus did not protect against subsequent infection with the cherry yellows virus. Mixed infections with the necrotic ring spot and prune dwarf viruses did not cause cherry yellows.


2009 ◽  
Vol 15 (1-2) ◽  
Author(s):  
H. G. Davarynejad ◽  
J. Nyéki ◽  
E. Davarynejad ◽  
T. Szabó ◽  
Z. Szabó

At the experiment orchard of the Fruit Research and Extension Institute, Újfehértó, Hungary in spring 2000 a trial was designed included: Erdi bőtermő, Debreceni bőtermő, and Csengődi sour cherry (Prunus avium L) cultivars which were budded at 1999 on Oblacsinska sour cherry (as a dwarf inter stock), which had been budded on Prunus mahaleb seedlings at 1998 with the aim to investigate the dwarfing effects of Oblacsinska sour cherry cultivars on scion cultivars. Trees of each cultivar directly on Prunus mahaleb seedling, severd as controls. Use of Oblacsinska as an interstock reduced the rootstock trunk diameter. Trees of Debreceni bőtermő, and Cseng6di sour cherry cultivars with Oblacsinska interstock were smaller than similar trees grafted directly onto Prunus mahaleb as a rootstock. Trees of Erdi bőtermő grafted on Oblacsinska interstock which buded on Prunus mahaleb were the smallest. Growth of the trees was reduced also by using of the Oblacsinszka as interstock for other examined cultivars.


2012 ◽  
Vol 59 (1) ◽  
pp. 471-477
Author(s):  
Stanisław Wociór ◽  
Marcin Kaca

In no examined years of the studies were found no significant differences of flowering intensity between the ownoroots trees of the cultivar Łutówka and trees budded on <i>Prunus mahaleb</i>. The kind of trees clearly, but not significantly, affected fruit mass. The growth of the ownroot "in vitro" trees were significantly less than the trees budded on <i>Prunus mahaleb</i> seedling. Fruit set and yielding of the own root trees were higher, but only in one year of study significantly higher.


Author(s):  
Dušica DORIĆ ◽  
Vladislav OGNJANOV ◽  
Mirjana LJUBOJEVIĆ ◽  
Goran BARAĆ ◽  
Jovana DULIĆ ◽  
...  

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