Pathogenity Test Bacteria Pasteurella multocida Local Isolate Using Postulate Koch

The study aimed to find out whether <em>Pasteurella multocida</em> bacteria isolated from buffalo in HSU (Hulu Sungai Utara) is the cause of SE (<em>Septicema epizootica</em>) disease in swamp buffaloes using Koch Postulates. Total of 10 Balb-C mice aged 2 weeks were infected with 100 μl culture containing 4 x 108 CFU (1.5 McFarland Scale) <em>P. multocida</em> subcutaneously in the neck , and observed every 4 hours until the animal died. Samples were taken from the spleen, lungs, and heart with different times of death within 15 hours, 35 hours, and 59 hours with sterile swab cotton. Samples were grown on a nutrient broth medium (NB), inoculated on a soy trypticase agar (TSA), and incubated for 24 hours at 37 ° C. Separate colonies were stained with Gram and spore staining. The colonies were tested by catalase, biochemical, indol motility (SIM) sulfite, confectionary, and planted on Mac Conkey Agar media. <em>P. multocida</em> was identified following Carter's method of showing lung, spleen, and positively infected <em>P. multocida</em> samples. It was concluded that <em>P. multocida</em> bacteria isolated from buffalo in HSU are pathogenic and can cause SE disease.

PRODUKSI MASAL INOKULUM AZOTOBACTER, AZOSPIRILLUM DAN BAKTERI PELARUT FOSFAT DENGAN MENGGUNAKAN MEDIA ALTERNATIF

<p>Azotobacter, Azospirillum and phosphate solubilizing bacteria are the most common microbial inoculants used as biofertilizer. To have good quality of biofertilizer, the high number of inoculant cells and suitable carriers as well as the method of carrier sterilization are among the most important factors determined the quality of biofertilizer. Related to the number of inoculant cells in carriers, the growing medium used to cultivate the microbial cells play very important role. For mass production of microbial cells, the medium should be able to support fast growth of microbial cells. The price of medium should be reasonably cheap and the materials used in medium should be available easily. The purpose of this study was to obtain a cheap growing medium that can support high number of microbial inoculant cells and the components of the medium should be easyly obtain and the price is not expensive. The study was conducted at the Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB). The results showed that the medium IPB RI-1 was able to support the growth of 1010 cfu ml-1 Azotobacter, 108 cfu ml-1 Azospirillum and 109 cfu ml-1 Phosphate Solubilizing Bacteria. The number of bacterial cells in Nutrient Broth medium was only 108 cfu ml-1. This means that the IPB RI-1 medium was able to produce 100-fold population of Azotobacter compared to the growth of this bacterium in Nutrient Broth and Phosphate Solubilizing Bacteria was 10-fold higher than population in Nutrient Broth medium. The costs of the IPB RI-1 and IPB RI-2 were much cheaper compared to the cost of Nutrient Broth medium. The cost of medium IPB RI-1 only 3% (IDR 945) and IPB RI-2 about 2% (IDR 690) of the cost of Nutrient Broth medium (IDR 27,752) per liter medium in the year of 2010.<br />Keywords : Alternative media, Azotobacter, Azospirillum, Nutrient Broth, Phosphate Solubilizing Bacteria</p>

BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623 percent and accumulate Cu in the cell 0.1149–0.1400 percent/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.