scholarly journals Pathogenity Test Bacteria Pasteurella multocida Local Isolate Using Postulate Koch

2019 ◽  
Vol 6 (1) ◽  
pp. 27
Author(s):  
Herliani Herliani ◽  
Abrani Sulaiman ◽  
Muhammad Ilmi Hidayat ◽  
Ananda Mellyani Hidayat
Keyword(s):  
Pasteurella Multocida ◽  
Nutrient Broth ◽  
Nutrient Broth Medium ◽  
Local Isolate ◽  
Swamp Buffaloes ◽  
Agar Media ◽  
Koch Postulates

The study aimed to find out whether <em>Pasteurella multocida</em> bacteria isolated from buffalo in HSU (Hulu Sungai Utara) is the cause of SE (<em>Septicema  epizootica</em>) disease in swamp buffaloes using Koch Postulates. Total of 10 Balb-C mice aged 2 weeks were infected with 100 μl culture containing 4 x 108 CFU (1.5 McFarland Scale) <em>P. multocida</em> subcutaneously in the neck , and observed every 4 hours until the animal died. Samples were taken from the spleen, lungs, and heart with different times of death within 15 hours, 35 hours, and 59 hours with sterile swab cotton. Samples were grown on a nutrient broth medium (NB), inoculated on a soy trypticase agar (TSA), and incubated for 24 hours at 37 ° C. Separate colonies were stained with Gram and spore staining. The colonies were tested by catalase, biochemical, indol motility (SIM) sulfite, confectionary, and planted on Mac Conkey Agar media. <em>P. multocida</em> was identified following Carter's method of showing lung, spleen, and positively infected <em>P. multocida</em> samples. It was concluded that <em>P. multocida</em> bacteria isolated from buffalo in HSU are pathogenic and can cause SE disease.

scholarly journals Toleransi logam berat timbal (Pb) pada bakteri indigenous dari air laut Pelabuhan Paotere, Makassar (Heavy metal lead [Pb] tolerance of indigenous bacteria from Seawater in Paotere Port, Makassar)

2020 ◽  
Vol 8 (1) ◽  
pp. 8
Author(s):  
Fahruddin Fahruddin ◽  
Slamet Santosa ◽  
Sareda .
Keyword(s):  
Heavy Metals ◽  
Nutrient Broth ◽  
Nutrient Agar ◽  
Resistant Bacteria ◽  
Bacterial Isolation ◽  
Sediment Samples ◽  
Marine Life ◽  
Agar Media ◽  
Seawater Samples ◽  
Polluted Waters

Marine water which has been polluted by heavy metals such as lead (Pb) from port activities can affect marine life, however several microorganisms are able to adapt in polluted waters due to their resistant capabilities. The research aimed to obtain isolates bacterial which are resistant to Pb and to test their resistant ability. Bacterial isolation was obtained from sediment and seawater taken in Paotere Port, Makassar. The isolation of the resistant bacteria was done using nutrient agar media which contained 10 ppm of Pb. The growth of the bacteria was initially observed macroscopically and microscopically to determine type of the colony. Resistant test for each type of isolate was done by inoculating them on nutrient broth media which contained different concentration of Pb: 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, and a control. The test resulted in eight different isolates, five isolates from sediment samples and three from seawater samples. The results showed that each isolate has different degree of resistant toward Pb. Isolate IA1 has the higher resistant ability, and it was followed by isolate IS5.---Perairan laut tercemar logam berat timbal (Pb), yang bersumber dari aktifitas pelabuhan, dapat mengganggu kehidupan biota laut, namun sejumlah mikrorganisme mampu beradaptasi karena memiliki kemampuan resistan.  Penelitian ini bertujuan untuk memperoleh isolat bakteri yang resistan terhadap Pb dan menguji kemampuan resistansinya. Isolasi bakteri diperoleh dari sampel sedimen dan air laut dari Pelabuhan Paotere, Makassar.Untuk isolasi bakteri resistan menggunakan media nutrient agar yang megandung 10 ppm Pb. Bakteri yang tumbuh diamati secara makropskopis dan mikroskopis sebagai pendekatan awal untuk mengetahui jenis isolat. Uji resistansi dilakukan untuk setiap jenis isolat, yang diinokulasikan pada media nutrient broth pada konsentrasi Pb sebesar 5 ppm, 10 ppm, 15 ppm. 20 ppm, 25 ppm dan 0 ppm sebagai kontrol.  Hasilnya, diperoleh delapan jenis isolat yang berbeda, yaitu lima jenis isolat dari sampel sedimen dan tiga dari sampel air laut. Dari hasil uji memperlihatkan, bahwa setiap isolat memiliki kemampuan resistansi berbeda-beda terhadap Pb. Isolat IA1 kemampuan resistansinya lebih tinggi, kemudian isolat IS5.

scholarly journals PRODUKSI MASAL INOKULUM AZOTOBACTER, AZOSPIRILLUM DAN BAKTERI PELARUT FOSFAT DENGAN MENGGUNAKAN MEDIA ALTERNATIF

2010 ◽  
Vol 12 (2) ◽  
pp. 33
Author(s):  
Richard Gunawan ◽  
Iswandi Anas ◽  
Fahrizal Hazra
Keyword(s):  
Nutrient Broth ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Cells ◽  
Microbial Cells ◽  
Nutrient Broth Medium ◽  
Growing Medium ◽  
Materials Used ◽  
Phosphate Solubilizing ◽  
The Cost

<p>Azotobacter, Azospirillum and phosphate solubilizing bacteria are the most common microbial inoculants used as biofertilizer. To have good quality of biofertilizer, the high number of inoculant cells and suitable carriers as well as the method of carrier sterilization are among the most important factors determined the quality of biofertilizer. Related to the number of inoculant cells in carriers, the growing medium used to cultivate the microbial cells play very important role. For mass production of microbial cells, the medium should be able to support fast growth of microbial cells. The price of medium should be reasonably cheap and the materials used in medium should be available easily. The purpose of this study was to obtain a cheap growing medium that can support high number of microbial inoculant cells and the components of the medium should be easyly obtain and the price is not expensive. The study was conducted at the Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB). The results showed that the medium IPB RI-1 was able to support the growth of 1010 cfu ml-1 Azotobacter, 108 cfu ml-1 Azospirillum and 109 cfu ml-1 Phosphate Solubilizing Bacteria. The number of bacterial cells in Nutrient Broth medium was only 108 cfu ml-1. This means that the IPB RI-1 medium was able to produce 100-fold population of Azotobacter compared to the growth of this bacterium in Nutrient Broth and Phosphate Solubilizing Bacteria was 10-fold higher than population in Nutrient Broth medium. The costs of the IPB RI-1 and IPB RI-2 were much cheaper compared to the cost of Nutrient Broth medium. The cost of medium IPB RI-1 only 3% (IDR 945) and IPB RI-2 about 2% (IDR 690) of the cost of Nutrient Broth medium (IDR 27,752) per liter medium in the year of 2010.<br />Keywords : Alternative media, Azotobacter, Azospirillum, Nutrient Broth, Phosphate Solubilizing Bacteria</p>

scholarly journals BIOAKUMULASI LOGAM BERAT Cu OLEH Bacillus sp

2004 ◽  
Vol 10 (1) ◽  
pp. 19-23
Author(s):  
Riesta Primaharinastiti ◽  
A. Toto Poernomo ◽  
Noor Erma S.
Keyword(s):  
Atomic Absorption ◽  
Room Temperature ◽  
Atomic Absorption Spectrophotometry ◽  
Nutrient Broth ◽  
Bacillus Sp ◽  
Accumulation Process ◽  
Absorption Spectrophotometry ◽  
Nutrient Broth Medium

The research was conducted to investigate the ability of Bacillus sp in accumulating Cu and how much it can be acumulated. The medium used to growth the bacterium was Nutrient Broth and Atomic Absorption Spectrophotometry methods was used to assay the Cu, both in the cells and medium. The result of this study showed that Bacillus sp incubated in the Nutrient Broth medium containing 10 ppm of Cu, with continuous stirring in the room temperature was able to reduce Cu in the medium 8.912–12.623 percent and accumulate Cu in the cell 0.1149–0.1400 percent/mg cells. Based on this result, it is necessary to develop more studies to find out what factors that influence the accumulation process and to optimize the bioprocess.

scholarly journals An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Anucha Muenthaisong ◽  
Boondarika Nambooppha ◽  
Amarin Rittipornlertrak ◽  
Pallop Tankaew ◽  
Thanya Varinrak ◽  
...  
Keyword(s):  
Immune Response ◽  
Pasteurella Multocida ◽  
Stimulation Index ◽  
Clinical Signs ◽  
Control Group ◽  
Protective Ability ◽  
Cell Mediated Immune Response ◽  
Swamp Buffaloes ◽  
Serotype B ◽  
Cellular Immune

Hemorrhagic septicemia (HS) is an important infectious disease in cattle and buffaloes, caused by Pasteurella multocida B:2 and E:2. The intranasal recombinant OmpH-based vaccine was successfully used to protect dairy cattle from HS in a previous study. Thus, this study aimed to examine the protective ability of that vaccine among buffaloes. Four groups of Thai swamp buffaloes received different vaccines and were labeled as 100 or 200 μg of the rOmpH with CpG-ODN2007, commercial HS bacterin vaccine, and nonvaccinated control groups. Sera and whole blood were collected to examine the antibody levels and cellular immune response using indirect ELISA and MTT assay, respectively. Challenge exposure was performed with virulent P. multocida strain M-1404 serotype B:2 on day 72 of the experiment. The antibody titers to P. multocida among immunized buffaloes were significantly higher than in the control group (p<0.01), especially the 200 μg of the rOmpH group. The stimulation index (SI) of the intranasally vaccinated groups revealed significantly higher levels than the nonvaccinated group (p<0.01), but not different from the intramuscularly commercial HS vaccine. The clinical signs and high fever were observed after challenge exposure in the nonvaccinated group, while it was not observed among the 200 μg of rOmpH immunized buffaloes. The other immunized groups showed partial protection with transient fever. In conclusion, the rOmpH-based intranasal vaccine could elicit protective ability and induce antibody- and cell-mediated immune response against virulent P. multocida strain among swamp buffaloes.

scholarly journals Potency of Cell Wall Protein of Pasteurella multocida as Hemorrhagic Septicemia Vaccine on Swamp Buffaloes

2020 ◽  
Vol 8 (1) ◽  
pp. 33
Author(s):  
Herliani , ◽  
Abrani Sulaiman ◽  
M Ilmi Hidayat
Keyword(s):  
Cell Wall ◽  
Pasteurella Multocida ◽  
Vaccine Candidate ◽  
Clinical Symptoms ◽  
Brain Heart Infusion ◽  
Vaccine Safety ◽  
Elisa Test ◽  
Safety Testing ◽  
Safety Test ◽  
Swamp Buffaloes

Vaccine candidate tests were carried out in the Laboratory of Microbiology, Faculty of Agriculture ULM; and the Laboratory of Molecular Genetics, Faculty of Science and Technology Airlangga University. The field test was conducted in Tampakang Village, Hulu Sungai Utara district and Bati-Bati village, Tanah Laut district that was including sterility test through vaccine mediated <em>Brain Heart Infusion </em>(BHI) inoculation. Safety testing was applied in experimental animals (mice and swamp buffaloes). Serum was tested serologically using <em>Enzyme Linked Immuno Sorbent Assay</em> (ELISA). Vaccine safety test showed 100% of mice and buffalo are surviving without clinical symptoms according to the characteristics of hemorrhagic septicemia disease. ELISA test indicated vaccinations increase antibody production, namely the <em>Optical Density</em> (OD) ƛ 450 ƞm 0.292 before vaccination to be 0.748 and 1.576 after vaccination and to be 1.821 after boosted. Based on sterilization test, safety test, and vaccine potency test, the vaccine candidate from cell wall proteins of <em>P. multocida</em> local isolate can be used to prevent hemorrhagic septicemia disease that infect swamp buffalo both on laboratory and field experiment.

Rapid Cultural Methods for Detection of Salmonella in Feeds and Feed Ingredients

1983 ◽  
Vol 46 (10) ◽  
pp. 851-855 ◽  
Author(s):  
J.-Y. D'AOUST ◽  
A. SEWELL ◽  
A. BOVILLE
Keyword(s):  
Standard Method ◽  
Brilliant Green ◽  
Nutrient Broth ◽  
Sample Analysis ◽  
Feed Ingredients ◽  
Selective Enrichment ◽  
Cultural Methods ◽  
Agar Media ◽  
Reduced Water ◽  
Single Medium

Efficacy of standard, 6-h standard and direct enrichment methods for detection of Salmonella in naturally contaminated feeds and feed ingredients was compared. Analysis by the standard method involved preenrichment of feed slurries in nutrient broth, selective enrichment in tetrathionate brilliant green (43°C) and selenite cystine (35°C), and isolation of presumptive isolates on bismuth sulfite and brilliant green sulfa agar media. Sample analysis by the 6-h standard method was identical to the above except that incubation of enrichment broths was reduced to 6 h; for direct enrichment. preenrichment in nutrient broth was omitted. Of 287 samples tested, 75 were found to contain salmonellae by the three methods combined. Ability of the standard and 6-h standard methods to identify the same 58 contaminated samples underlines the reliability of the 6-h standard method for the more rapid detection of Salmonella in animal feeds. Identification of 68 positive samples by direct enrichment presumably resulted from equilibration (3 to 4 h) of feed slurries at reduced water activity before analysis. Addition of novobiocin (40 μg/ml) to selective enrichment broths did not facilitate isolation of Salmonella through repression of competitive flora. Productivity of the six enrichment-plating combinations used in this study was comparable, and no single medium played a determinant role in recovery.

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