scholarly journals PRODUKSI MASAL INOKULUM AZOTOBACTER, AZOSPIRILLUM DAN BAKTERI PELARUT FOSFAT DENGAN MENGGUNAKAN MEDIA ALTERNATIF

2010 ◽  
Vol 12 (2) ◽  
pp. 33
Author(s):  
Richard Gunawan ◽  
Iswandi Anas ◽  
Fahrizal Hazra

<p>Azotobacter, Azospirillum and phosphate solubilizing bacteria are the most common microbial inoculants used as biofertilizer. To have good quality of biofertilizer, the high number of inoculant cells and suitable carriers as well as the method of carrier sterilization are among the most important factors determined the quality of biofertilizer. Related to the number of inoculant cells in carriers, the growing medium used to cultivate the microbial cells play very important role. For mass production of microbial cells, the medium should be able to support fast growth of microbial cells. The price of medium should be reasonably cheap and the materials used in medium should be available easily. The purpose of this study was to obtain a cheap growing medium that can support high number of microbial inoculant cells and the components of the medium should be easyly obtain and the price is not expensive. The study was conducted at the Department of Soil Science and Land Resources, Faculty of Agriculture, Bogor Agricultural University (IPB). The results showed that the medium IPB RI-1 was able to support the growth of 1010 cfu ml-1 Azotobacter, 108 cfu ml-1 Azospirillum and 109 cfu ml-1 Phosphate Solubilizing Bacteria. The number of bacterial cells in Nutrient Broth medium was only 108 cfu ml-1. This means that the IPB RI-1 medium was able to produce 100-fold population of Azotobacter compared to the growth of this bacterium in Nutrient Broth and Phosphate Solubilizing Bacteria was 10-fold higher than population in Nutrient Broth medium. The costs of the IPB RI-1 and IPB RI-2 were much cheaper compared to the cost of Nutrient Broth medium. The cost of medium IPB RI-1 only 3% (IDR 945) and IPB RI-2 about 2% (IDR 690) of the cost of Nutrient Broth medium (IDR 27,752) per liter medium in the year of 2010.<br />Keywords : Alternative media, Azotobacter, Azospirillum, Nutrient Broth, Phosphate Solubilizing Bacteria</p>

2021 ◽  
Vol 2094 (4) ◽  
pp. 042070
Author(s):  
Salokhiddin Mardonov ◽  
Laziz Shokirov ◽  
Khakim Rakhimov

Abstract In this article, in order to reduce the consumption of starch for gluing warp threads and reduce the cost of sizing materials used in sizing and improve the quality of threads, an effective gluing composition was created based on a hydrolyzed acrylic emulsion (GAE) and uzkhitan (chitosan and carboxymethyl cellulose), that is, corn starch polymer and studied their rheological properties. In the article, the main threads passing through the gluing process and moving at a certain speed, which are then impregnated with liquid gluing. Then they pass through a nip roller and a drying drum, are subjected to friction and form a layer of a certain thickness. A theoretical analysis of the surface of the warp threads is carried out to determine the change in the diameter of the threads when a layer of a certain thickness is formed on their surface.


2021 ◽  
Vol 410 ◽  
pp. 254-261
Author(s):  
Aleksandr I. Cherepanov ◽  
Viktor A. Kukartsev ◽  
Vladislav V. Kukartsev

One of the effective ways to improve the quality of semi-finished products made from aluminum alloys is to eliminate the columnar and fan-shaped structure in them, refine the grain and achieve homogeneity, is modification and alloying. Modification of the melt is carried out using ligatures and allows a significant increase in the casting rate without fear of an excessive increase in the degree of zonal segregation during crystallization, as well as ensuring the uniformity of the chemical composition over the section. An important role in the quality of modification is also played by the manufacturing technology of the master alloy itself, which should ensure an increase in the cooling rate during crystallization. To obtain an alloy with the required properties, the quality of the charge materials used must be considered. First of all, this concerns master alloys, which are used for alloying and modifying the alloy. The most common for the manufacture of ingots and shaped castings are master alloys containing boron or boron and titanium. The boron content in these ligatures is 1-5%. It is generally accepted that a large amount of boron (except for the rise in the cost of the alloy itself) upon accelerated cooling promotes the refinement of the internal structure of the grain, but can lead to an increase in large inclusions of TiB2.


2019 ◽  
Vol 4 (1) ◽  
pp. 134
Author(s):  
Yuni Sri Rahayu

Petroleum is a mixture of hydrocarbon complexes with organic compounds from sulfur, oxygen, nitrogen and metal-containing compounds. These organic compounds can be used as substrate for bacterial growth. This study aimed to isolate and identify hydrocarbon degrading bacteria and phosphate solubilizing bacteria in oil-contaminated soil in Bojonegoro. This study used an exploration method to find each of the two types of hydrocarbon degrading bacteria and phosphate solubilizing bacteria from soil samples in Bojonegoro that contaminated by oil. Identification of isolates bacterial included macroscopic observations of bacteria, gram staining on bacterial cells and physiological tests. Macroscopic observations include the form of colonies, colony diameter, colony color, colony edge, and elevation. The physiological test using Microbact Identification System to determine the physiological characteristics of bacteria so that genera and types of bacteria can be known. The identification of organisms was based on changes in pH and use of the substrate. The results of data analysis were obtained from five types of bacteria from soil samples that contaminated by oil which were successfully isolated. After identification of species was done, four species of bacteria were obtained, namely Pseudomonas pseudomallei, Pseudomonas fluorescens-25, Flavobacterium odoratum, and Enterococcus sp.


2013 ◽  
Vol 295-298 ◽  
pp. 2328-2332 ◽  
Author(s):  
Shu Qing Zhang ◽  
Jian Feng Li ◽  
Shang Li Shi ◽  
Ping Hui Huo ◽  
Wu Wu Wen ◽  
...  

Phosphorus is the main nutrient element for plant growth, whereas most of phosphate in soil is fixated by calcium, aluminum, iron and organic compounds. Available phosphate deficiency has been the main limiting factor that influences the yield and quality of agricultural products. P solubilizing microorganisms have been widely reported of solubilizing different forms of insoluble phosphates. P solubilizing Rhizobium, especially for strains with the ability of secreting 3-Indole acetic acid, which is also capable of fixating N and inoculation, is found excellent in promoting growth and yield of legume crops, which decreases the cost of agricultural production and the side effect of P application to the environment. The mechanism and amount range of P solubilization for Rhizobium are varied among strains. These results and phenomena from different studies are discussed in this paper.


2020 ◽  
Author(s):  
Tamanreet Kaur

Urbanization and industrialization resulted in rapid increase in volume of solid waste; its management has become one of the biggest problems today. Solid wastes can be disposed off by methods like land filling, incineration, conversion into biogas, recycling, and composting, but its overproduction has led to inappropriate disposal practices such as their indiscriminate and inappropriately timed application to agricultural fields that ultimately leads to water and soil pollution. However, if handled properly, these organic wastes can be used for vermicomposting; it is an effective recycling technology that improves the quality of the products which is disinfected, detoxified, and highly nutritive. It is a low cost, eco-biotechnological process of waste management in which earthworms are used to cooperate with microorganisms in order to convert biodegradable wastes into organic fertilizer. Earthworms excreta (vermicast) is a nutritive organic fertilizer rich in humus, NPK, micronutrients, beneficial soil microbes; nitrogen-fixing, phosphate solubilizing bacteria, actinomycets, and growth hormones auxins, gibberlins and cytokinins, is a suitable alternative to chemical fertilizers, being an excellent growth promoter and protector for crop plants. Thus, vermiculture not only results in management of soild waste but also produces excellent nutrient enriched vermicompost. Vermicompost is beneficial for sustainable organic agriculture and maintaining balanced ecosystem.


Author(s):  
K. Sreedevi Shankar ◽  
K. Suma ◽  
K. Bhuvana Chary ◽  
A. Prem Kumar ◽  
Minakshi Grover ◽  
...  

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