Sedimentary ancient DNA reveals a threat of warming-induced alpine habitat loss to Tibetan Plateau plant diversity

Studies along elevational gradients worldwide usually find the highest plant taxa richness in mid-elevation forest belts. Hence, an increase in upper elevation diversity is expected in the course of warming-related treeline rise. Here, we use a time-series approach to infer past taxa richness from sedimentary ancient DNA from the south-eastern Tibetan Plateau over the last ~18,000 years. We find the highest total plant taxa richness during the cool phase after glacier retreat when the area contained extensive and diverse alpine habitats (14–10 ka); followed by a decline when forests expanded during the warm early- to mid-Holocene (10–3.6 ka). Livestock grazing since 3.6 ka promoted plant taxa richness only weakly. Based on these inferred dependencies, our simulation yields a substantive decrease in plant taxa richness in response to warming-related alpine habitat loss over the next centuries. Accordingly, efforts of Tibetan biodiversity conservation should include conclusions from palaeoecological evidence.

Absence of blood parasites and other vector-borne pathogens in Alpine marmots (Marmota marmota) in Western Austria

The importance of vectors and vector-borne diseases (VBDs) is increasing on a global scale. Many vectors and pathogens benefit from global warming and can spread to novel habitats where they were formerly not present, including higher altitudes. Various vector-borne pathogens (VBPs), such as Anaplasma phagocytophilum, have been reported in, for instance, red foxes and wild ungulates in the Western Austrian Alps. However, these animals are known to migrate to lower regions in the winter season, and therefore, it is of interest to investigate if VBPs are also present in mammals faithful to their higher altitude alpine habitat all year round. Blood parasites and other VBPs, namely. Trypanosomatidae, piroplasms, Hepatozoon spp., filarioid helminths, Anaplasmataceae, and Rickettisa spp., were thus analysed with PCR in 148 alpine marmots (Marmota marmota). None of the marmots’ blood samples was positive for these VBPs, indicating a low abundance or absence of competent vectors in the alpine region. Alpine marmots seem to be naïve for VBPs (at least in our study area). An overview of VBD agents in other marmot species is given.

Jungle Cat Felis chaus Schreber, 1777 (Mammalia: Carnivora: Felidae) at high elevations in Annapurna Conservation Area, Nepal

To date, the Jungle Cat Felis chaus has been recorded in the lowlands and mid-hills in Nepal. Photographic evidence, however, is scarce, particularly in alpine habitat. Here we present the first photographic records of the Jungle Cat in an alpine habitat in Lower Mustang of the Annapurna Conservation Area, Nepal. It was recorded by camera traps at elevations of 3,000–3,100 m in 2014 and 2016 in 10 detections during 2,755 trap days. In July 2016, one individual was sighted at an elevation of 3,300m. The habitat of these records comprised riverbed, a poultry farm adjacent to agricultural land and shrubland of Juniper and Caragana species.

Comparing Primary and Secondary Growth of Co-Occurring Deciduous and Evergreen Conifers in an Alpine Habitat

Investigations on primary and secondary growth in co-occurring species will aid in assessment of the physiological adaptation of species and the prediction of forest stand structure dynamics. To explore the correlation and coordination between primary and secondary growth, we monitored the leaf phenology, shoot elongation, and stem growth of co-occurring Larix principis-rupprechtii Mayr. and Picea meyeri Rehd. et Wils. in an alpine habitat, Luya Mountain (North-Central China), during the growing season of 2014. We measured bud development on terminal branches three days per week by direct observations and intra-annual stem xylem formation at weekly intervals by the microcores method. The onset sequence of three organs was the needle, shoot, and stem, without species-specific differences. Needles appeared one month earlier than stem growth in larch, while it was only one week earlier in spruce. The duration of needle growth was the shortest, followed by the shoot and stem. The timing of primary growth (i.e., onset, end, and maximum growth rate) between the two species was asynchronous, but secondary growth was synchronic with the same date of the maximum growth rate occurrence, potentially indicating species competition for resources. Unlike larch, spruce staggered growth peaks among different organs, which may effectively mitigate trees’ internal competition for resources. Soil temperature was positively correlated with both shoot and stem growth in the two species, whereas air temperature and soil water content were positively correlated with needle growth only in larch. Therefore, it can be inferred that the spruce will probably outcompete the larch at cold alpine treeline sites due to its high adaptability to acquiring and allocating resources. These results provide insight into the potential physiological correlation between primary and secondary growth and allow better prediction of future climate change effects on forest ecosystem productivity.

The genus Hebeloma in the Rocky Mountain Alpine Zone

Numerous taxa ofHebelomahave been reported in association withSalix,Dryas, andBetulain arctic-alpine habitats. However, species are notoriously difficult to delineate because morphological features overlap, and previously there was little reliable molecular data available. Recent progress in ITS-sequencing within the genus, coupled with an extensive database of parametrically described collections, now allows comparisons between species and their distributions. Here we report 16 species ofHebelomafrom the Rocky Mountain alpine zone from some of the lowest latitudes (latitude 36°–45°N) and highest elevations (3000–4000 m) for arctic-alpine fungi in the northern hemisphere. Twelve of these species have been reported from arctic-alpine habitats in Europe and Greenland and are now molecularly confirmed from the Middle and Southern Rockies, greatly expanding their distribution. These are:Hebelomaalpinum,H.aurantioumbrinum,H.dunense,H.hiemale,H.marginatulum,H.mesophaeum,H.nigellum,H.oreophilum,H.subconcolor,H.spetsbergense,H.vaccinum, andH.velutipes. Hebelomahygrophilumis known from subalpine habitats in Europe, but was never recorded in arctic-alpine ecology. Three species recorded from the Rockies, but as yet not reported from Europe, areH.alpinicola,H.avellaneum, andH.excedens. The last two have never previously been reported from an arctic-alpine habitat. For all three of these species, the holotypes have been studied morphologically and molecularly, and have been incorporated into the analysis.

The First Record for Altai Fescue, Festuca altaica (Poaceae), in Nova Scotia, from an Eastern Alpine Site on Cape Breton Island

Altai Fescue, Festuca altaica Trinius, is an amphi-Beringian grass species also known from isolated, but widespread, locations in northeastern North America. The occurrence reported here, at the southern limit of eastern alpine habitat in Canada, represents the first for Nova Scotia.