Microorganisms mediate soil organic carbon (SOC) turnover, and microbial residues contribute a significant portion to SOC storage in temperate agroecosystems. However, little is known about the direct effect of temperature on microbial residues associated with SOC sequestration/decomposition. We assessed microbial residue dynamics in a 28 d incubation conducted at four temperatures (5, 15, 25, and 35 °C). Microbial residues did not change with time from 5 to 25 °C. However, at 35 °C, fungal residues decomposed significantly with time, and the decomposition rate was higher than SOC. Considering the important contribution of fungal residues to stable-C pool, our findings indicated warming may be detrimental to C stability in this temperate soil.