Phytostabilization of Polluted Military Soil Supported by Bioaugmentation with PGP-Trace Element Tolerant Bacteria Isolated from Helianthus petiolaris

Lead (Pb) and cadmium (Cd) are major environmental pollutants, and the accumulation of these elements in soils and plants is of great concern in agricultural production due to their toxic effects on crop growth. Also, these elements can enter into the food chain and severely affect human and animal health. Bioaugmentation with plant growth-promoting bacteria (PGPB) can contribute to an environmentally friendly and effective remediation approach by improving plant survival and promoting element phytostabilization or extraction under such harsh conditions. We isolated and characterised Pb and Cd-tolerant root-associated bacteria from Helianthus petiolaris growing on a Pb/Cd polluted soil in order to compose inoculants that can promote plant growth and also ameliorate the phytostabilization or phytoextraction efficiency. One hundred and five trace element-tolerant rhizospheric and endophytic bacterial strains belonging to eight different genera were isolated from the aromatic plant species Helianthus petiolaris. Most of the strains showed multiple PGP-capabilities, ability to immobilise trace elements on their cell wall, and promotion of seed germination. Bacillus paramycoides ST9, Bacillus wiedmannii ST29, Bacillus proteolyticus ST89, Brevibacterium frigoritolerans ST30, Cellulosimicrobium cellulans ST54 and Methylobacterium sp. ST85 were selected to perform bioaugmentation assays in greenhouse microcosms. After 2 months, seedlings of sunflower (H. annuus) grown on polluted soil and inoculated with B. proteolyticus ST89 produced 40% more biomass compared to the non-inoculated control plants and accumulated 20 % less Pb and 40% less Cd in the aboveground plant parts. In contrast, B. paramycoides ST9 increased the bioaccumulation factor (BAF) of Pb three times and of Cd six times without inhibiting plant growth. Our results indicate that, depending on the strain, bioaugmentation with specific beneficial bacteria can improve plant growth and either reduce trace element mobility or enhance plant trace element uptake.

Multiple chromosomal inversions contribute to adaptive divergence of a dune sunflower ecotype

ABSTRACTBoth models and case studies suggest that chromosomal inversions can facilitate adaptation and speciation in the presence of gene flow by suppressing recombination between locally adapted alleles. Until recently, however, it has been laborious and time-consuming to identify and genotype inversions in natural populations. Here we apply RAD sequencing data and newly developed population genomic approaches to identify putative inversions that differentiate a sand dune ecotype of the prairie sunflower (Helianthus petiolaris) from populations found on the adjacent sand sheet. We detected seven large genomic regions that exhibit a different population structure than the rest of the genome and that vary in frequency between dune and non-dune populations. These regions also show high linkage disequilibrium and high heterozygosity between, but not within haplotypes, consistent with the behavior of large inversions, an inference subsequently validated in part by comparative genetic mapping. Genome-environment association analyses show that key environmental variables, including vegetation cover and soil nitrogen, are significantly associated with inversions. The inversions co-locate with previously described “islands of differentiation,” and appear to play an important role in adaptive divergence and incipient speciation within H. petiolaris.

Cold Stress Tolerance during Early Growth Stages of Naturalized Helianthus petiolaris Populations

In the last 10 years the sunflower crop area of Argentina has shifted towards the Southwest as a result of the expansion of soybean cultivation. Early sowings require greater tolerance to low temperatures in order to optimize the use of water resources by the crop, especially in the seedling stage, when cold stress limits growth and productivity. Helianthus petiolaris is a wild genetic resource that has contributed to the production of sunflower cultivars. The goal of this study was to evaluate the tolerance to low temperatures in H. petiolaris plants. Morphological traits (plant height, leaf width and leaf length) and physiological traits (electrolyte leakage, chlorophyll content, and glucose content) were compared in eight accessions having an early germination in the field and one with normal life cycle. Acclimated and non-acclimated plants were subjected to cold stress followed by a recovery period. Physiological traits during early growth stages showed differences in cold tolerance and for acclimation ability between accessions, suggesting the existence of heritable components. Four accessions were the most cold-tolerant among the studied populations, suggesting a natural adaptation related to the geographical site. Results point out that H. petiolaris is a useful genetic resource to contribute to sunflower breeding in the search of cold tolerant cultivars.