A procedure is outlined for the isolation
of pure anthocyanins and their identification without recourse to comparison
with reference glycosides. The isolation involves cellulose and polyamide
column chromatography and the identification depends on simple but selective
oxidative and hydrolytic reactions, adapted to a microscale using paper
chromatography.
By this method the anthocyanin pigments
present in extracts of black currant fruit have been unequivocally identified
as cyanidin and delphinidin, their 3-glucosides and 3-rutinosides.
Epigenetic regulation of osteoblastogenesis by blackcurrant fruit extracts in vitro and in vivo
