Capnocytophaga periodontitidis sp. nov., isolated from subgingival plaque of periodontitis patient

Two carbon dioxide-requiring, gliding, Gram-stain-negative strains, designated p1a2T and 051621, were isolated from subgingival plaque in association with severe periodontitis. The 16S rRNA gene sequence analysis revealed that they represented members of the genus Capnocytophaga and had less than 96.4 % pairwise similarity with species with validly published names in this genus. The whole-genome sequences of those strains had less than 91.9 % average nucleotide identity and 48.4 % digital DNA–DNA hybridization values with the other type strains of species of the genus Capnocytophaga , both below the species delineation threshold. The results of pan-genomic analysis indicated that p1a2T and 051621 shared 765 core gene families with the other ten species in this genus, and the numbers of strain-specific gene families were 493 and 455, respectively. The major fatty acids were iso-C15 : 0 and C16 : 0. A combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicate that p1a2T and 051621 should be considered to represent a novel species of the genus Capnocytophaga , for which the name Capnocytophaga periodontitidis sp. nov. is proposed. The type strain is p1a2T (=CGMCC 1.17337T=JCM 34126T).

FROM TRIVIAL TO CONSEQUENTIAL-A UNIQUE APPROACH OF BUTTRESS BONE AS AN AUTO-GRAFT FOR EFFECTIVE RESTORATION

An unusual bony growth evokes suspicion, buttressing bone formation is one such exemplar. Periodontal disease leads to the destruction of soft and hard supporting tissue. The immunoinflammatory process in bone triggers the formation and resorption of bone simultaneously. In an attempt to buttress bony trabecular weakened by resorption, peripheral buttressing bone is formed which sometimes is accompanied by the formation of intrabony defect. In the present case report, A 27-year-old female patient presented with the chief complaint of painless hard swelling on the upper right and left-back tooth region since 5-6 years. The patient was diagnosed with generalized chronic Periodontitis with Bilateral Maxillary buttressing of bone. Conventional Respective osseous surgery was done in one quadrant and respective osseous surgery with the regenerative technique by using the bone obtained during surgery as an autograft to fill the intraosseous defect in the same periodontitis patient. The patient was followed up after 3 weeks, 3 months, and then 6 months. There was no increase in bone growth at the end of the 6th month obtaining the bone as a graft from donor site during the respective osseous procedure serves dual advantage of Recon touring and obtaining desirable bony contours and eliminating any other donor site or another kind of graft material for restoring the intraosseous defect. As in the case presented, the problem was used as a solution. The buttress bone obtained from the respective procedure serves as the gold standard autogenous graft for restoring the intraosseous defect.

POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC

Background: Chronic periodontitis, a chronic inflammatory condition of periodontal tissue, can occur due to microorganisms as a local factor and genetic as a systemic factor. Genetic factors that cause chronic periodontitis, namely mutations in the form of a gene that functions to regulate calcium homeostasis, which is called the vitamin D receptor (VDR) gene. Mutated VDR gene causing different effect in a population and ethnic groups, including Javanese Banyumas Ethnic. Purpose: To understand the genotype distribution and VDR BsmI (rs1544410) gene allotype frequency chronic periodontitis patient in Javanese Banyumas Ethnic. Method: The research was descriptive research in the form of a cross-sectional study of 26 chronic periodontitis patients in the Javanese Banyumas tribe. The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. Result: Whole samples from DNA isolation amplificated with one ribbon 524 bp VDR gene. RFLP restriction enzyme Bsml process is also showing cutting in each sample. From data analysis, X2 is 0,44 with p=0,50 (p>0,05) resulted. It was showing mutant VDR gene allele frequency in chronic periodontitis patients in Javanese Banyumas ethnic were balanced genetically. Conclusion: Genotype distribution in chronic periodontitis patient in Javanese Banyumas ethnic were 20 (76,9%) GG (bb) genotype, 6 (23,1%) GA (Bb) genotype, and 0 (0%) AA (BB) genotype with alel G (b) (wild type) allele frequency 0,88 (88%) and A allele (B or mutant) 0,12 (12%).

Orthodontic treatment of an adult patient with aggressive periodontitis – A case report

A 26-year-old man with an aggressive periodontitis sought for orthodontic treatment to improve the appearance of his smile. He presented with generalised anterior spacing, missing lowerleft central incisor and deep traumatic bite. He was treated successfully with a combination of orthodontic and periodontal treatment. After 18 months of orthodontic treatment and follow upby the periodontist, his alignment of teeth was improved, a stable occlusion was achieved, and occlusal trauma was prevented. As a result, the patient’s smile appearance and selfconfidence were improved. Orthodontic tooth movement is not only to correct the alignment of his teeth but also to improve the bone level especially at the anterior region. This case report shows the successful treatment outcome in aggressive periodontitis patient which requires good collaboration between the orthodontist and the periodontist.