Response of Three Miscanthus × giganteus Cultivars to Toxic Elements Stress: Part 1, Plant Defence Mechanisms

Miscanthus × giganteus demonstrated good phytostabilization potentials in toxic element (TE) contaminated soils. However, information about its tolerance to elevated concentrations is still scarce. Therefore, an ex-situ pot experiment was launched using three cultivars (termed B, U, and A) grown in soils with a gradient Cd, Pb and Zn concentrations. Control plants were also cultivated in non-contaminated soil. Results show that the number of tillers per plant, stem diameter as well as leaf photosynthetic pigments (chlorophyll a, b and carotenoids) were negatively impacted by soil contamination. On the other hand, phenolic compounds, flavonoids, tannins, and anthocyanins levels along with the antioxidant enzymatic activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase increased in the plants grown on contaminated soils. Altogether, these data demonstrate that miscanthus is impacted by concentrations of toxic elements yet is able to tolerate high levels of soil contamination. These results may contribute to clarifying the miscanthus tolerance strategy against high contamination levels and its efficiency in phytoremediation.

Placental Immune Tolerance and Organ Transplantation: Underlying Interconnections and Clinical Implications

The immune system recognizes and attacks non-self antigens, making up the cornerstone of immunity activity against infection. However, during organ transplantation, the immune system also attacks transplanted organs and leads to immune rejection and transplantation failure. Interestingly, although the embryo and placenta are semi-allografts, like transplanted organs, they can induce maternal tolerance and be free of a vigorous immune response. Also, embryo or placenta-related antibodies might adversely affect subsequent organ transplantation despite the immune tolerance during pregnancy. Therefore, the balance between the immune tolerance in maternal-fetal interface and normal infection defense provides a possible desensitization and tolerance strategy to improve transplantation outcomes. A few studies on mechanisms and clinical applications have been performed to explore the relationship between maternal-fetal immune tolerance and organ transplantation. However, up to now, the mechanisms underlying maternal-fetal immune tolerance remain vague. In this review, we provide an overview on the current understanding of immune tolerance mechanisms underlying the maternal-fetal interface, summarize the interconnection between immune tolerance and organ transplantation, and describe the adverse effect of pregnancy alloimmunization on organ transplantation.

Contrasting Water Use, Stomatal Regulation, Embolism Resistance, and Drought Responses of Two Co-Occurring Mangroves

The physiological mechanisms underlying drought responses are poorly documented in mangroves, which experience nearly constant exposure to saline water. We measured gas exchange, foliar abscisic acid (ABA) concentration, and vulnerability to embolism in a soil water-withholding experiment of two co-occurring mangroves, Avicennia marina (Forsskål) Vierhapper (Verbenaceae) and Bruguiera gymnorrhiza (L.) Savigny (Rhizophoraceae). A. marina showed higher photosynthesis and transpiration than B. gymnorrhiza under well-watered conditions. Cavitation resistance differed significantly between species, with 50% cavitation occurring at a water potential (P50) of −8.30 MPa for A. marina and −2.83 MPa for B. gymnorrhiza. This large difference in cavitation resistance was associated with differences in stomatal closure and leaf wilting. The rapid stomatal closure of B. gymnorrhiza was correlated with ABA accumulation as water potential declined. Meanwhile, stomatal closure and declining water potentials in A. marina were not associated with ABA accumulation. The safety margins, calculated as the difference between stomatal closure and embolism spread, differed between these two species (1.59 MPa for A. marina vs. 0.52 MPa for B. gymnorrhiza). Therefore, A. marina adopts a drought tolerance strategy with high cavitation resistance, while B. gymnorrhiza uses a drought avoidance-like strategy with ABA-related sensitive stomatal control to protect its vulnerable xylem.

Increasing WSN Lifetime using Clustering and Fault Tolerance Methods

Energy consumption problems in wireless sensor networks are an essential aspect of our days where advances have been made in the sizes of sensors and batteries, which are almost very small to be placed in the patient's body for remote monitoring. These sensors have inadequate resources, such as battery power that is difficult to replace or recharge. Therefore, researchers should be concerned with the area of saving and controlling the quantities of energy consumption by these sensors efficiently to keep it as long as possible and increase its lifetime. In this paper energy-efficient and fault-tolerance strategy is proposed by adopting the fault tolerance technique by using the self-checking process and sleep scheduling mechanism for avoiding the faults that may cause an increase in power consumption as well as energy-efficient at the whole network. this is done by improving the LEACH protocol by adding these proposed strategies to it. Simulation results show that the recommended method has higher efficiency than the LEACH protocol in power consumption also can prolong the network lifetime. In addition, it can detect and recover potential errors that consume high energy.

Typha Latifolia L. Grown in Carrying Petroleum Secondary Effluent Supply Nutrients Enhance for Biomass Growth and Phytoremediation of Heavy Metals and TPH

Phytoremediation is an innovative tool which can be used for the treatment of industrial and agricultural wastewater. Typha latifolia L. (T. latifolia L) is an aquatic plant which grows on petroleum secondary effluent (PSE) containing metals like cadmium (Cd), cobalt (Co), manganese (Mn) and TPH (total petroleum hydrocarbon). The growth performance in biomass, nutrient concentrations and heavy metals in parts of the T. latifolia L. The reason for the accumulation of Cd, Co and Mn in T. latifolia L. can be explained as a tolerance strategy due to its transfer index (TI) which is higher than 2.9. The enrichment coefficients of the metals present in the root compared to stem of T. latifolia L. were higher than 3.31 to 2.56 for Cd, 5.35 to 3.55 Co. But, for Mn were found to be lower 1.98 than 3.51 at 75%. Similarly, the enrichment coefficients of all the metals, except for Co, in roots of T. latifolia L. were higher than 5.36. (TI) for Co (2.95) and Mn (2.55) which is absolutely better as compared to the enrichment coefficients of Cd (2.35) and TPH (3.45) in PSE. Thus, there is a possibility that PSE could be a source of important nutrients.

Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage

High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. Myroides profundi D25T, obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine N-oxide (TMAO) by a TMA monooxygenase, MpTmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-MpTmm pathway was further confirmed by introducing it into Escherichia coli and Bacillus subtilis. Encoded TmaT-like and MpTmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and MpTmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.

Salinity Duration Differently Modulates Physiological Parameters and Metabolites Profile in Roots of Two Contrasting Barley Genotypes

Hordeum maritimum With. is a wild salt tolerant cereal present in the saline depressions of the Eastern Tunisia, where it significantly contributes to the annual biomass production. In a previous study on shoot tissues it was shown that this species withstands with high salinity at the seedling stage restricting the sodium entry into shoot and modulating over time the leaf synthesis of organic osmolytes for osmotic adjustment. However, the tolerance strategy mechanisms of this plant at root level have not yet been investigated. The current research aimed at elucidating the morphological, physiological and biochemical changes occurring at root level in H. maritimum and in the salt sensitive cultivar Hordeum vulgare L. cv. Lamsi during five-weeks extended salinity (200 mM NaCl), salt removal after two weeks of salinity and non-salt control. H. maritimum since the first phases of salinity was able to compartmentalize higher amounts of sodium in the roots compared to the other cultivar, avoiding transferring it to shoot and impairing photosynthetic metabolism. This allowed the roots of wild plants to receive recent photosynthates from leaves, gaining from them energy and carbon skeletons to compartmentalize toxic ions in the vacuoles, synthesize and accumulate organic osmolytes, control ion and water homeostasis and re-establish the ability of root to grow. H. vulgare was also able to accumulate compatible osmolytes but only in the first weeks of salinity, while soon after the roots stopped up taking potassium and growing. In the last week of salinity stress, the wild species further increased the root to shoot ratio to enhance the root retention of toxic ions and consequently delaying the damages both to shoot and root. This delay of few weeks in showing the symptoms of stress may be pivotal for enabling the survival of the wild species when soil salinity is transient and not permanent.