EFFECT OF PROVENANCE AND WATER STRESS ON BIOMASS AND POLYPHYLLIN CONTENT IN THE MEDICINAL PLANT Paris polyphylla Smith var. yunnanensis

Water stress and provenance could affect the secondary metabolites synthesis and accumulation in herbs. Thus, this study explored the effect of soil water moisture and provenance on the growth of Paris polyphylla Smith var. yunnanensis (PPY). Three provenances (Jinping, Luquan and Weixi in Yunnan, China) of PPY samples were grown in different soil water moisture conditions [0.80, 0.70 and 0.50 field capacity (FC)] during Dec. 2015 to Sep. 2017. Results showed that the highest biomass weight was presented in 0.70 FC for Luquan and Weixi samples. Biomass weight for Jinping provenance presented a decreasing tendency with the decreased soil water moisture and the highest biomass were shown in 0.80 FC. However, quantitative analysis revealed that the total content of polyphyllin increased with decreasing the soil water moisture for Jinping and Weixi samples. The highest total content of polyphyllin in rhizome was inclined to show in Jinping samples, while the stem and leaf tissues were shown in Weixi samples. Additionally, results of ANOVA combined with PCA indicated that the difference among these three provenances were significant. Correlation analysis results revealed that 0.50 FC induced the competitive relationship occurrence for polyphyllin distribution. Thus, 0.70 FC was the most suitable soil-water condition for PPY growth. Besides, provenance collected from Jinping could consider as a good quality germplasm. Consequently, this study might provide a preliminary foundation for irrigation project formulated and provenance screened for PPY cultivation.

PRODUTO MODIS ASSOCIADO À CONDIÇÃO HÍDRICA NO SOLO PARA DIFERENCIAR PADRÕES NA VEGETAÇÃO NO MUNICÍPIO DE DOM ELISEU, PARÁ (MODIS PRODUCT ASSOCIATED WITH SOIL WATER CONDITION TO DISTINGUISH VEGETATION PATTERNS IN THE MUNICIPALITY OF DOM ELISEU, PARÁ)

Objetivou avaliar a dinâmica de padrões na vegetação usando NDVI (Normalized Difference Vegetation Index) associado à oferta hídrica no município de Dom Eliseu, no Pará com base na reflectância em áreas com cultivos anuais de grãos e plantios florestais, nos períodos de maior e menor deficiência de água no solo. Foram analisados dados meteorológicos para calcular balanços hídricos (CAD = 300 mm) e respostas em NDVI (Normalized Difference Vegetation Index) extraídos do sensor MODIS (Moderate Resolution Imaging Spectroradiometer). As imagens-índice (NDVI) referentes aos meses de janeiro a dezembro de 2012 foram processadas no aplicativo Envi 4.7 e reclassificadas no ArcGIS10.1. Os resultados apontaram variações temporais ao longo do ano, tanto relacionados aos sistemas de agrícolas de produção, quanto aos remanescentes florestais os quais indicavam associações à oferta hídrica na região e possíveis respostas fenológicas. Em Dom Eliseu, o mês de maior valor em NDVI foi em abril com mais 60% do município expressando manutenção das folhas e da capacidade fotossintética das plantas, pois os valores em NDVI foram superiores a 0,6. No período de agosto a setembro ocorrem as menores cotas pluviais, ocasionando déficits hídricos que atingem valores superiores a 70 mm. Observou-se que as respostas em NDVI foram mais expressivas no mês de outubro, totalizando 16% da área de estudo com valores entre 0,2 a 0,3, evidenciando reduzida expressão em resposta espectral na biomassa dos remanescentes de vegetação e plantios florestais. Conclui-se que existe sensibilidade do NDVI em resposta à condição hídrica no solo. Ao contabilizar-se as diferenças entre a reflectâncias no infravermelho próximo e no vermelho divididos pela soma dessas reflectância, os baixos valores de NDVI, reforçam que no período de maior deficiência hídrica há queda de folhas, pois a superfície imageada, responde com valores mais elevados no solo do que na vegetação.

Interactions between soybean and weeds in a replacement series system, considering the effects of water stress

Due to the increase of water deficiency in many farm regions and its meaning on weed interference, competitive interactions between soybean and three weeds were evaluated under water stress (20 to 40 days after transplanting) and no stress conditions. Three independent experiments were carried out in a growth chamber, being each one composed by the weeds Alternanthera tenella, Tridax procumbens or Digitaria ciliaris, along with the crop, in which soil water condition and plant composition effects were evaluated while in competition. A replacement series system was used, including both monoculture of each species and a mixture with a ratio of 50% between weed and soybean. A completely randomized design was used in factorial arrangement, with treatments distributed in three levels for plant composition factor (soybean and weeds monocultures, in addition to the soybean + weed mixture) and two levels for the water factor (with or without stress), amounting six treatments in each experiment. Soybean dry mass was higher than weed dry mass, when growing without water stress. However, under water stress conditions, the dry mass of soy was reduced in all experiments, mainly in the D. ciliaris comparative experiment. Water restriction was also significant in the plants' photosynthesis reduction in most of the experiments, reducing leaf area duration and efficiency of water use. Analysing all variables shows greater weed tolerance than soybean when submitted to water deficit and with distinct changes of their interactions and mechanism of competition, in each experiment.

Temporal and spatial variations of soil carbon dioxide, methane, and nitrous oxide fluxes in a Southeast Asian tropical rainforest

To clarify the factors controlling temporal and spatial variations of soil carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) fluxes, we investigated these gas fluxes and environmental factors in a tropical rainforest in Peninsular Malaysia. Temporal variation of CO2 flux in a 2-ha plot was positively related to soil water condition and rainfall history. Spatially, CO2 flux was negatively related to soil water condition. When CO2 flux hotspots were included, no other environmental factors such as soil C or N concentrations showed any significant correlation. Although the larger area sampled in the present study complicates explanations of spatial variation of CO2 flux, our results support a previously reported bipolar relationship between the temporal and spatial patterns of CO2 flux and soil water condition observed at the study site in a smaller study plot. Flux of CH4 was usually negative with little variation, resulting in the soil at our study site functioning as a CH4 sink. Both temporal and spatial variations of CH4 flux were positively related to the soil water condition. Soil N concentration was also related to the spatial distribution of CH4 flux. Some hotspots were observed, probably due to CH4 production by termites, and these hotspots obscured the relationship between both temporal and spatial variations of CH4 flux and environmental factors. Temporal variation of N2O flux and soil N2O concentration was large and significantly related to the soil water condition, or in a strict sense, to rainfall history. Thus, the rainfall pattern controlled wet season N2O production in soil and its soil surface flux. Spatially, large N2O emissions were detected in wet periods at wetter and anaerobic locations, and were thus determined by soil physical properties. Our results showed that, even in Southeast Asian rainforests where distinct dry and wet seasons do not exist, variation in the soil water condition related to rainfall history controlled the temporal variations of soil CO2 flux, CH4 uptake, and N2O emission. The soil water condition associated with soil hydraulic properties was also the important controlling factor of the spatial distributions of these gas fluxes.