Improving Particle Collection Efficiency of Sampling Wipes used for Trace Chemical Detection

Improvement of the particle collection efficiency of sampling wipes is desirable for optimizing the performance of many wipe-based chemical analysis techniques used for trace chemical screening applications. In this note,...

Experimental Investigation of the Effects of Helical Roof on the Performance of Stairmand High Efficiency Cyclone

Cost-effectiveness of a standard and a modified Stairmand high-efficiency type cyclone was compared at various inlet gas velocities. The modified design was obtained by replacing the roof of the standard design with a helical roof. Experiments were conducted by both standard and helical roof designs at the same operating conditions. Results showed that helical roof leads to reduced pressure drop in cyclones while having a negative impact on particle collection efficiency. Reductions in pressure drop can reach up to 30%, while particle collection efficiency is reduced by up to 8% simultaneously. Overall, the treatment cost of a cyclone separator can be reduced by 14.1–20.8%. Results indicated that helical roof design cyclones can be used to reduce overall treatment cost by cyclone separators.

Particle Collection in Imhoff Sedimentation Cones Enriches Both Motile Chemotactic and Particle-Attached Bacteria

Marine heterotrophic microorganisms remineralize about half of the annual primary production, with the microbiomes on and around algae and particles having a major contribution. These microbiomes specifically include free-living chemotactic and particle-attached bacteria, which are often difficult to analyze individually, as the standard method of size-selective filtration only gives access to particle-attached bacteria. In this study, we demonstrated that particle collection in Imhoff sedimentation cones enriches microbiomes that included free-living chemotactic bacteria and were distinct from particle microbiomes obtained by filtration or centrifugation. Coastal seawater was collected during North Sea phytoplankton spring blooms, and the microbiomes were investigated using 16S rRNA amplicon sequencing and fluorescence microscopy. Enrichment factors of individual operational taxonomic units (OTUs) were calculated for comparison of fractionated communities after separation with unfractionated seawater communities. Filtration resulted in a loss of cells and yielded particle fractions including bacterial aggregates, filaments, and large cells. Centrifugation had the lowest separation capacity. Particles with a sinking rate of >2.4 m day–1 were collected in sedimentation cones as a bottom fraction and enriched in free-living chemotactic bacteria, i.e., Sulfitobacter, Pseudoalteromonas, and Vibrio. Subfractions of these bottom fractions, obtained by centrifugation, showed enrichment of either free-living or particle-attached bacteria. We identified five distinct enrichment patterns across all separation techniques: mechano-sensitive and mechano-stable free-living bacteria and three groups of particle-attached bacteria. Simultaneous enrichment of particle-attached and chemotactic free-living bacteria in Imhoff sedimentation cones is a novel experimental access to these groups providing more insights into the diversity, structure, and function of particle-associated microbiomes, including members of the phycosphere.

Design and Analysis of a Separator for Aluminium Dust Particle Collection

Cyclone separators are considered one of the most common and widely used method of separating solid particles from fluids. The focus of this paper was to design a separator that could effectively improve the air quality of an industrial working environment by considering factors such as the nature of the particles and the restrictions on the device such as size and operating condition. To do so, a sample of the dust sample was studied to determine the characteristics of the aluminum dust. An initial separator model which satisfied the separation condition was developed, and a second model was developed with the goal of optimizing and reducing the overall size of the separator. All models developed were subjected to CFD simulations to analyze the flow patterns, separation rate at various particle diameters as well as comparing against the theoretical predicted values. The simulation results showed how the change in various parameters improved the separation efficiency. The total height of the model was approximately 20% smaller than the initial proposed design to fit into the manufacturer’s workbench while the separation efficiencies improved by an average of 5% to 10% across various particle sizes. The results obtained indicated that the efficiency of the particle collection is dependent on the area of the barrel inlet of the separator and the depth of the vortex finder.

How do Planktonic Particle Collection Methods Affect Bacterial Diversity Estimates and Community Composition in Oligo-, Meso- and Eutrophic Lakes?

Particles are hotspots of bacterial growth and nutrient recycling in aquatic ecosystems. In the study of particle-attached (PA) and/or free-living (FL) microbial assemblages, the first step is to separate particles from their surrounding water columns. Widely used collection techniques are filtration using different pore size filters, and centrifugation; however, it is unclear how the bacterial diversity, bacterial community structure (BCS) and taxonomic composition of PA assemblages are affected by different particle collection methods. To address this knowledge gap, we collected planktonic particles from eutrophic Lake Taihu, mesotrophic Lake Tianmu, and oligotrophic Lake Fuxian in China, using filtration with five pore size of filters (20, 10, 8.0, 5.0, and 3.0 μm), and centrifugation. Bacterial communities were then analyzed using Illumina MiSeq sequencing of the 16S rRNA gene. We found that PA collection method affected BCS significantly in all lakes. Centrifugation yielded the highest species diversity and lowest mean percentage of photoautotrophic Cyanobacteria in Lake Taihu, but not in the other two lakes, thus highlighting the potential compatibility of this method in the study of PA assemblage in eutrophic lakes. The high bacterial diversity and low relative percentage of Cyanobacteria was in samples retained on 5.0 μm filters in all lakes. These results suggest that collecting PA samples in lakes using filters with 5.0 μm pore size is the preferred protocol, if species diversity and heterotrophic bacteria are the top research priorities, when comparing bacterial communities in different trophic lakes at the same time. The present study offers the possibility of collecting PA samples using unified methods in oligotrophic to eutrophic lakes.