Contribution to the knowledge of Chinese Gryllacrididae (Orthoptera) IV: New additions of Chinese Furcilarnaca Gorochov, 2004

Based on the specimens from Guangxi and Yunnan deposited in the Guangxi Normal University, the paper is firstly reported the female sex of two known species from China, viz. Furcilarnaca armata (Bey-Bienko, 1957) and Furcilarnaca forceps (Bey-Bienko, 1962). The female of Furcilarnaca armata (Bey-Bienko, 1957) is very similar to Furcilarnaca chiangdao in seventh abdominal tergite and subgenital plate, and they may belong to the same species. After examining the female subgenital plate of Furcilarnaca chirurga (Bey-Bienko, 1962), we treat Furcilarnaca hirta as a valid species. Mitochondrial genomes of six specimens of three Furcilarnaca species were sequenced and annotated in this study. The organization and gene content of new sequenced mitogenomes were conserved, except for significant variation of the control region. All the PCGs started with the typical ATN codons and most ended with complete TAA or TAG codons. Combined with five mitogenomes in NCBI, a total of twelve specimens (including one outgroup) were used in phylogenetic analysis based on 13 PCGs. The result revealed that Furcilarnaca was a monophyletic group. These data will provide more meaningful genetic information and validate the phylogenetic relationships within the Gryllacrididae.

Systematics of the American Katydids (Orthoptera: Tettigoniidae). Communication 10

A new material on the Neotropical genus Anaulacomera Stål, 1873 from its subgenera Anallomes Stål, 1875, Stenotegmora subgen. nov., Irisovia subgen. nov. and Munticercora Gorochov, 2020 is considered with description of six new species and one new subspecies: A. (Anallomes) galarzai sp. nov. from Peru (Junin Department: Satipo Prov.); A. (A.) monicae sp. nov. from Peru (Junin Department: Satipo Prov.); A. (S.) adunca sp. nov. from Peru (Junin Department: Satipo Prov.); A. (S.) spinifera sp. nov. from Peru (Ucayali Department: Atalaya Prov.); A. (S.) originalis sp. nov. from Peru (Junin Department: Satipo Prov.); A. (I.) grigoryi sp. nov. from Peru (Junin Department: Satipo Prov.); A. (M.) sclerogenitalis woronovi subsp. nov. from Colombia (“Penas Blancos, Rio Magdalena”). The above-mentioned new subgenera are distinguished from all the other subgenera of this genus by the following features: Stenotegmora subgen. n. by narrower tegmina, and the male genitalia with a large median inflation on the dorsal fold; Irisovia subgen. n. by the male last abdominal tergite with strongly denticulate posterior lobes, the male cerci short but thick and with distal processes having numerous spinule-like setae, and the male genitalia membranous. For A. (M.) psedoepiproctalis Gorochov, 2020 and A. (M.) s. sclerogenitalis Gorochov, 2020, some important geographical mistakes in their type data are corrected (Ecuador and Mexico instead Peru, respectively), and for the latter taxon, a previously unknown female is described.

Revision of the genera Habetia Kirby, 1906 and Parahabetia gen. nov. from New Guinea (Orthoptera, Tettigoniidae, Conocephalinae, Agraeciini)

The genus Habetia Kirby, 1906 previously known from only four species or subspecies is revised based on unidentified specimens found in museum collections. H. multispinulosa Griffini, 1908 is given full species status, while H. pictifrons Karny, 1911 is removed from Habetia based on male and female characters and becomes the type species of the new genus Parahabetia gen. nov. Sixteen new species are described in Habetia, and one new species and two new subspecies in Parahabetia. The most significant characters for the differentiation of species of Habetia are male tenth abdominal tergite, cerci, subgenital plate, and male internal genitalia with the titillators that basically form a pair of roughly U-shaped sclerites that vary between species from simple, curved sclerites to modified structures with additional sclerotized branches and the sclerites can be connected with septa to form complex shapes. In contrast, males of Parahabetia have elongate titillators provided at end with a crest. In the larger species of Habetia the stridulatory file on the underside of the male left tegmen has a step in pre-apical area that is missing in the smaller species; one species with a weak step is intermediate between both groups. Unique modifications of single species in Habetia are an apical dorsal extension of the male subgenital plate in H. imitatrix Karny, 1912 and a hardened phallus sheath between phallus and subgenital plate in H. tuta sp. nov. Females of Habetia have straight ovipositors that vary in length and in the shape of the dorsal margin between species. Females can also be differentiated by species specific modifications of the subgenital plate. In Parahabetia the ovipositor is rather short and curved similar to the condition in the genus Philmontis Willemse, 1966 but has the apical area slightly recurved. Keys to the species of both genera are provided. Habetia species have been found throughout New Guinea except so far for Papua Barat; although they were mainly found in the North and the East. Parahabetia species are currently only known from the East of New Guinea. The following species and subspecies are described as new: Habetia bivittata sp. nov., H. curvata sp. nov., H. dentata sp. nov., H. elevata sp. nov., H. kondiu sp. nov., H. lalibu sp. nov., H. nigricauda sp. nov., H. pallida sp. nov., H. pedala sp. nov., H. pilleata sp. nov., H. pinnigera sp. nov., H. quatrispina sp. nov., H. simbai sp. nov., H. sororcula sp. nov., H. tuta sp. nov., H. wau sp. nov., Parahabetia bispinosa sp. nov., P. pictifrons obtusa ssp. nov., P. pictifrons acuta ssp. nov.

Revision of the genus Attaphila (Blattodea: Blaberoidea), myrmecophiles living in the mushroom gardens of leaf-cutting ants

The genus Attaphila, comprising minute myrmecophilous cockroaches, is revised, including now six previously known (A. aptera, A. bergi, A. flava, A. fungicola, A. schuppi, A. sexdentis) and three new species (A. multisetosasp. nov. Bohn and Klass, A. paucisetosasp. nov. Bohn and Klass, A. sinuosocarinatasp. nov. Bohn and Klass). All species are described or redescribed and depicted with their main characteristics; determination keys allow the identification of males and females. Especially the male characters allow a distribution to two species-groups with differing host specificity: bergi-group associated with Acromyrmex (and possibly Amoimyrmex) ants, fungicola-group associated with Atta ants; the former appears paraphyletic, the latter monophyletic. The genus Attaphila is characterised emphasising its unique features: (1) insertion of antennae at the bottom of a wide funnel-shaped deepening; (2) antenna with the possibility of a rectangular bending between scapus and pedicellus (associated with a distal excavation of the scapus) and (3) with an unusual shape and low number of antennomeres; (4) femora of legs with a ventral groove allowing a close spacing of femur and tibia during a strong flexion; (5) a complex and unusual shape of the laterosternal shelf area of the female genitalia (lack of shelf, presence of a pair of complicated tubular invaginations); and (6) lateral parts of abdominal tergite T9 of male ending in a pair of ventromesally directed arms, which contact the lateral margins of the subgenital plate. Functional aspects and the possible biological roles of these features are discussed. Older biological data are summarised and new observations are presented. The position of Attaphila within Blattodea is discussed. Like a recent molecular study, the morphology of the male genitalia places the genus in the Blaberoidea. The molecular result of Attaphila being closest to three particular blattellid genera, however, is conflictual from the morphological perspective.

A new species of Anaulacomera Stål, 1873 (Orthoptera, Tettigoniidae, Phaneropterinae) from Parque Nacional de Itatiaia, Southeastern Brazil

Anaulacomera currently contains 147 valid species, of those 50 are recorded from Brazil. The genus is further divided into six subgenera, which can be distinguished on characteristics of the male terminalia. A new Brazilian species is presently proposed, Anaulacomera (Anallomes) curvata sp. nov., based on three males and one female collected at Parque Nacional de Itatiaia, Itatiaia, Rio de Janeiro State. It can be distinguished from all other species because of the pair of cerciform projections of the tenth male abdominal tergite and a combination of shape and size of the male cerci.

A new species of Heminicsara (Orthoptera: Tettigoniidae: Agraeciini) from West Papua (New Guinea)

Heminicsara Karny, 1912 is a katydid genus of Agraeciini from the Axylus genus group. It currently comprises 62 species from mainly New Guinea and surrounding archipelagos. Based on recent fieldwork in Lobo in West Papua, Indonesia, a new species of Heminicsara is described here: Heminicsara incrassata sp. nov. It is most readily characterised from congeners and other species of the Axylus genus group by the male tenth abdominal tergite forming a large shield-shaped plate. This represents the first species of Heminicsara described and known from the south-west of New Guinea.

SOME LINEAR MEASUREMENTS OF HONEY-BEE LIVINGIN THE VICINITY OF THE CITY OF BARNAUL OF THE ALTAI REGION

The indices used in determining bee breeds were stud-ied: wing size and abdominal tergite 3 width; the metatar-sus of the right hind leg was measured to determine the tarsal index. The percentage of individuals with different variants of discoidal displacement (positive, negative, neu-tral) was determined. The data of morphometric measure-ments of bees of 3 colonies are presented. The wing indi-ces in the 1st group of bees were slightly lower thanthe standard values (8.81-9.21; 3.01-3.23 mm). In the 2nd and 3rd bee colonies, the wing length was the same as in the European darkand Carpathian populations in 15% and 25% of individuals. A certain number of insects (15; 25; 60%) had a wing width equalto the European darkand Italian races. The studied bees had the sizes of the third abdominal tergite (4.88 ± 0.030; 4.92 ± 0.030; 4.97 ± 0.033 mm) as in all major breeds kept in the Russian Fed-eration. The tarsal index values (60.43 ± 0.452; 58.62 ± 0.445; 57.08 ± 0.322%) did not fit within the standards of the main bee breeds (54.0-56.2%). There was yellow color of the third tergite (35%; 80%) and all types of discoidal displacement (+; -; 0) in the insects of the 1st and 2nd col-onies. In the 3rd bee colony, the yellowness of the ab-dominal tergites lacked completely, and the positive dis-coidal displacement made 80%. In 3 studied groups of bees, the coefficient of variability was at the level of 1-4% for all indices. Thus, the research revealed the insectswith the signs of the European dark, Carniolan, Carpathian, Italian and Caucasian breeds.

Morphology and taxonomic status of Aedes aegypti populations across Senegal

Aedes aegypti is the primary vector of dengue, Zika, yellow fever and chikungunya viruses to humans. In Africa, two subspecies, Ae. aegypti aegypti (Aaa) and Ae. aegypti formosus (Aaf) have been described. Until very recently, it was considered that the two forms were sympatric in East Africa and that only Aaf was present in Central and West Africa. However, recent data suggests that Aaa was also common in Senegal without any clear evidence of genetic differences with Aaf. This study was carried out in different Ae. aegypti populations from Senegal to better clarify their taxonomic status. The larvae, pupae and eggs were collected between July and September 2018 and reared individually to adult stage. For each population, F1 progeny from eggs laid by a single female F0 were reared as sibling samples. The number of pale scales on the first abdominal tergite (T1) and the basal part of the second tergite (T2) were counted. Individuals with no pale scale on T1 were classified as Aaf while those with at least one pale scale on this tergite were classified as Aaa. The morphological variations within families of Aaf were studied across 4 generations. In total, 2400 individuals constituting 240 families were identified, of which 42.5% were heterogeneous (families with both forms). Multivariate statistical analysis of variance including T1 and T2 data together showed that populations were significantly different from each other. Statistical analysis of T1 alone showed a similarity between populations from the southeast while variations were observed within northwest population. The analysis of family composition across generations showed the presence of Aaa and Aaf forms in each generation. The classification of Ae. aegypti into two subspecies is invalid in Senegal. Populations exhibit morphological polymorphism at the intra-family level that could have biological and epidemiological impacts.

A new species of the genus Sinohaplotropis Cao et Yin, 2008 (Orthoptera, Acridoidea, Pamphagidae) from Amur River, Russia

The genus Sinohaplotropis Cao et Yin, 2008 is a valid genus and not a synonym of Haplotropis Saussur, 1888. A new species Sinohaplotropis amurensis sp. nov. is described in this paper from Russia. The new species is similar to Sinohaplotropis erenchuanensis Cao et Yin, 2008, but differs from latter by subgenital plate of male triangular, with large bifurcate apex in dorsal view, pronotum longer reaching first abdominal tergite, top of Kraus` organ narrow and interspace of mesosternum straight in the middle. Type specimen is deposited in the Hebei University, Baoding, Hebei, China.

Sexual Dimorphism in Anthonomus santacruzi (Coleoptera: Curculionidae): a Biological Control Agent of Solanum mauritianum Scopoli (Solanaceae)

There is evident variation in body size amongst Anthonomus santacruzi Hustache, 1924, weevils. The aims of this study were to assess if the variation in body size in A. santacruzi weevils is a result of sexual dimorphism and what features can be used to distinguish males from females. The weevils were collected from field sites in Mpumalanga, South Africa, where they were introduced as biocontrol agents of Solanum mauritianum Scopoli. Body structures and the presence/absence of the tergal notch was examined under an optical stereomicroscope and SEM to assess differences between sexes. The morphometric analysis of the body structures included rostrum length (base–apex and antennal insertion–apex), elytral length and width, pronotum length and width, first tarsus length, first tibia length, funiculus length and total body length. Rostrum length, elytra length and width and total body length were significantly larger in females than in males. A tergal notch in the 8th abdominal tergite was present in males and absent in females. The body structure; of rostrum length, elytra length and width and total body length overlapped between sexes in some specimens. The abdominal tergal notch was found to be the most useful body structure to distinguish males from females in A. santacruzi.