dna turnover
Recently Published Documents


TOTAL DOCUMENTS

45
(FIVE YEARS 0)

H-INDEX

16
(FIVE YEARS 0)

2012 ◽  
Vol 13 (5) ◽  
pp. 557-564 ◽  
Author(s):  
Suresh Kumar Poovathingal ◽  
Jan Gruber ◽  
LakshmiNarayanan Lakshmanan ◽  
Barry Halliwell ◽  
Rudiyanto Gunawan


Microbiology ◽  
2010 ◽  
Vol 156 (6) ◽  
pp. 1758-1771 ◽  
Author(s):  
A. de Morgan ◽  
L. Brodsky ◽  
Y. Ronin ◽  
E. Nevo ◽  
A. Korol ◽  
...  

Exponential-phase yeast cells readily enter stationary phase when transferred to fresh, carbon-deficient medium, and can remain fully viable for up to several months. It is known that stationary-phase prokaryotic cells may still synthesize substantial amounts of DNA. Although the basis of this phenomenon remains unclear, this DNA synthesis may be the result of DNA maintenance and repair, recombination, and stress-induced transposition of mobile elements, which may occur in the absence of DNA replication. To the best of our knowledge, the existence of DNA turnover in stationary-phase unicellular eukaryotes remains largely unstudied. By performing cDNA-spotted (i.e. ORF) microarray analysis of stationary cultures of a haploid Saccharomyces cerevisiae strain, we demonstrated on a genomic scale the localization of a DNA-turnover marker [5-bromo-2′-deoxyuridine (BrdU); an analogue of thymidine], indicative of DNA synthesis in discrete, multiple sites across the genome. Exponential-phase cells on the other hand, exhibited a uniform, total genomic DNA synthesis pattern, possibly the result of DNA replication. Interestingly, BrdU-labelled sites exhibited a significant overlap with highly expressed features. We also found that the distribution among chromosomes of BrdU-labelled and expressed features deviates from random distribution; this was also observed for the overlapping set. Ty1 retrotransposon genes were also found to be labelled with BrdU, evidence for transposition during stationary phase; however, they were not significantly expressed. We discuss the relevance and possible connection of these results to DNA repair, mutation and related phenomena in higher eukaryotes.



2008 ◽  
Vol 10 (3) ◽  
pp. 137-140 ◽  
Author(s):  
G. Zajicek ◽  
D. Schwartz-Arad
Keyword(s):  


2004 ◽  
Vol 286 (1-2) ◽  
pp. 97-109 ◽  
Author(s):  
Robert Busch ◽  
Denise Cesar ◽  
Dana Higuera-Alhino ◽  
Tracy Gee ◽  
Marc K Hellerstein ◽  
...  


Genetics ◽  
2002 ◽  
Vol 160 (1) ◽  
pp. 63-73
Author(s):  
Michael J McEachern ◽  
Dana Hager Underwood ◽  
Elizabeth H Blackburn

Abstract Telomerase adds telomeric DNA repeats to telomeric termini using a sequence within its RNA subunit as a template. We characterized two mutations in the Kluyveromyces lactis telomerase RNA gene (TER1) template. Each initially produced normally regulated telomeres. One mutation, ter1-AA, had a cryptic defect in length regulation that was apparent only if the mutant gene was transformed into a TER1 deletion strain to permit extensive replacement of basal wild-type repeats with mutant repeats. This mutant differs from previously studied delayed elongation mutants in a number of properties. The second mutation, TER1-Bcl, which generates a BclI restriction site in newly synthesized telomeric repeats, was indistinguishable from wild type in all phenotypes assayed: cell growth, telomere length, and in vivo telomerase fidelity. TER1-Bcl cells demonstrated that the outer halves of the telomeric repeat tracts turn over within a few hundred cell divisions, while the innermost few repeats typically resisted turnover for at least 3000 cell divisions. Similarly deep but incomplete turnover was also observed in two other TER1 template mutants with highly elongated telomeres. These results indicate that most DNA turnover in functionally normal telomeres is due to gradual replicative sequence loss and additions by telomerase but that there are other processes that also contribute to turnover.



2001 ◽  
Vol 15 (7) ◽  
pp. 1132-1138 ◽  
Author(s):  
MINGSHAN YAN ◽  
WENAN QIANG ◽  
NA LIU ◽  
JIANJUN SHEN ◽  
WILLIAM S. LYNN ◽  
...  


2000 ◽  
Vol 151 (1) ◽  
pp. 103
Author(s):  
S. Van Linthout ◽  
D. Collen ◽  
B. De Geest
Keyword(s):  
Tnf Α ◽  


1999 ◽  
Vol 17 (5) ◽  
pp. 314-319 ◽  
Author(s):  
Hasan Biri ◽  
H. Serdar Öztürk ◽  
Murat Kaçmaz ◽  
Kani Karaca ◽  
Haluk Tokuçoglu ◽  
...  


Sign in / Sign up

Export Citation Format

Share Document