Lensless dual-color fluorescence imaging device using hybrid filter

In this study, a dual-band hybrid filter that achieves high excitation light rejection performance in a lensless imaging system was fabricated and incorporated into an imaging device. The hybrid filter consisted of interference and absorption filters, and a fiber optic plate. The interference filters were attached to both sides of the fiber optic plate, which was placed on top of the absorption filter to suppress the decrease in spatial resolution. In addition, the lamination order was optimized to achieve a high fluorescence observation performance. The fabricated hybrid filter was mounted on an image sensor and had the ability to indicate the green and red fluorescence components.

MEASUREMENT OF PARAMETERS OF BROADBAND EMISSION USING MINI-SPECTROMETERS

On the basis of mini-spectrometers, a reference lamp of the SIRSH type with a known color temperature, and line spectrum sources, an inexpensive hardware complex has been created for measuring the emission parameters of heterodiodes and interference filters. Examples of recording the emission of heterodiodes (full width at half maximum is 17–30 nm) with a maximum of emission in the region of 659 and 764 nm and measurement of an interference filter (FWHM of the bandwidth is 12 nm) with a maximum transmission of 727 nm are given. The emission parameters of the SIRSH standard lamp are introduced into the program for measuring and processing data, due to which it is possible to significantly refine the value of the wavelength of the maximum emission and transmission.

Mitigation of bias sources for atmospheric temperature and humidity in the mobile Weather & Aerosol Raman Lidar (WALI)

Lidars using vibrational and rotational Raman scattering to continuously monitor both the water vapor and temperature profiles in the low and middle troposphere offer enticing perspectives for applications in weather prediction and studies of aerosol/cloud/water vapor interactions by deriving simultaneously relative humidity and atmospheric optical properties. Several heavy systems exist in European laboratories but only recently have they been downsized and ruggedized for deployment in the field. In this paper, we describe in detail the technical choices made during the design and calibration of the new Raman channels for the mobile Weather and Aerosol Lidar (WALI), going over the important sources of bias and uncertainty on the water vapor & temperature profiles stemming from the different optical elements of the instrument. For the first time, the impacts of interference filters and non-common-path differences between Raman channels, and their mitigation, are particularly investigated, using horizontal shots in a homogenous atmosphere. For temperature, the magnitude of the highlighted biases can be much larger than the targeted absolute accuracy of 1 °C defined by the WMO. Measurement errors are quantified using simulations and a number of radiosoundings launched close to the laboratory.