scholarly journals Lensless dual-color fluorescence imaging device using hybrid filter

2021 ◽  
Author(s):  
Natcha Kulmala ◽  
Kiyotaka Sasagawa ◽  
Thanaree Treepetchkul ◽  
Hironari Takehara ◽  
Makito Haruta ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Imaging System ◽  
Image Sensor ◽  
Dual Band ◽  
Hybrid Filter ◽  
Excitation Light ◽  
Imaging Device ◽  
Interference Filters ◽  
High Fluorescence ◽  
Absorption Filter

Abstract In this study, a dual-band hybrid filter that achieves high excitation light rejection performance in a lensless imaging system was fabricated and incorporated into an imaging device. The hybrid filter consisted of interference and absorption filters, and a fiber optic plate. The interference filters were attached to both sides of the fiber optic plate, which was placed on top of the absorption filter to suppress the decrease in spatial resolution. In addition, the lamination order was optimized to achieve a high fluorescence observation performance. The fabricated hybrid filter was mounted on an image sensor and had the ability to indicate the green and red fluorescence components.

scholarly journals Smartphone-Based Portable Bioluminescence Imaging System Enabling Observation at Various Scales from Whole Mouse Body to Organelle

Sensors ◽  
2020 ◽  
Vol 20 (24) ◽  
pp. 7166
Author(s):  
Mitsuru Hattori ◽  
Sumito Shirane ◽  
Tomoki Matsuda ◽  
Kuniaki Nagayama ◽  
Takeharu Nagai
Keyword(s):  
Light Source ◽  
Imaging System ◽  
High Sensitivity ◽  
Objective Lens ◽  
Portable Devices ◽  
Excitation Light ◽  
Imaging Device ◽  
Biological Targets ◽  
Color Changes ◽  
External Light Source

Current smartphones equipped with high-sensitivity and high-resolution sensors in the camera can respond to the needs of low-light imaging, streaming acquisition, targets of various scales, etc. Therefore, a smartphone has great potential as an imaging device even in the scientific field and has already been introduced into biomolecular imaging using fluorescence tags. However, owing to the necessity of an excitation light source, fluorescence methods impair its mobility. Bioluminescence does not require illumination; therefore, imaging with a smartphone camera is compact and requires minimal devices, thus making it suitable for personal and portable imaging devices. Here, we report smartphone-based methods to observe biological targets in various scales using bioluminescence. In particular, we demonstrate, for the first time, that bioluminescence can be observed in an organelle in a single living cell using a smartphone camera by attaching a detachable objective lens. Through capturing color changes with the camera, changes in the amount of target molecules was detected using bioluminescent indicators. The combination of bioluminescence and a mobile phone makes possible a compact imaging system without an external light source and expands the potential of portable devices.

scholarly journals Dual-band wavelength-swept active mode locking laser for multi-band fiber-optic sensors

2012 ◽  
Author(s):  
Hwi Don Lee ◽  
Chang Seok Kim ◽  
Myung Young Jeong ◽  
Zhongping Chen
Keyword(s):  
Fiber Optic ◽  
Mode Locking ◽  
Fiber Optic Sensors ◽  
Dual Band ◽  
Active Mode ◽  
Multi Band

scholarly journals A low-cost, human-like, high-resolution, tactile sensor based on optical fibers and an image sensor

2018 ◽  
Vol 15 (4) ◽  
pp. 172988141878363 ◽  
Author(s):  
Utku Büyükşahin ◽  
Ahmet Kırlı
Keyword(s):  
High Resolution ◽  
Spatial Resolution ◽  
Fiber Optic ◽  
Low Cost ◽  
Experimental Studies ◽  
Main Idea ◽  
Tactile Sensor ◽  
Image Sensor ◽  
Sensor Design ◽  
Tactile Sensors

Tactile sensors are commonly a coordinated group of receptors forming a matrix array meant to measure force or pressure similar to the human skin. Optic-based tactile sensors are flexible, sensitive, and fast; however, the human fingertip’s spatial resolution, which can be regarded as the desired spatial resolution, still could not be reached because of their bulky nature. This article proposes a novel and patented optic-based tactile sensor design, in which fiber optic cables are used to increase the number of sensory receptors per square centimeter. The proposed human-like high-resolution tactile sensor design is based on simple optics and image processing techniques, and it enables high spatial resolution and easy data acquisition at low cost. This design proposes using the change in the intesity of the light occured due to the deformation on contact/measurement surface. The main idea is using fiber optic cables as the afferents of the human physiology which can have 9 µm diameters for both delivering and receiving light beams. The variation of the light intensity enters sequent mathematical models as the input, then, the displacement, the force, and the pressure data are evaluated as the outputs. A prototype tactile sensor is manufactured with 1-mm spatial and 0.61-kPa pressure measurement resolution with 0–15.6 N/cm2 at 30 Hz sampling frequency. Experimental studies with different scenarios are conducted to demonstrate how this state-of-the-art design worked and to evaluate its performance. The overall accuracy of the first prototype, based on different scenarios, is calculated as 93%. This performance is regarded as promising for further developments and applications such as grasp control or haptics.

scholarly journals Development of a Compact,  Configurable, Real-Time Range  Imaging System

2021 ◽  
Author(s):  
◽  
Adrian Peter Paul Jongenelen
Keyword(s):  
High Speed ◽  
Imaging System ◽  
Modulation Frequency ◽  
Image Sensor ◽  
Measurement Precision ◽  
Operating Parameters ◽  
Range Imaging ◽  
Field Range ◽  
Full Field ◽  
Precision And Accuracy

<p>This thesis documents the development of a time-of-flight (ToF) camera suitable for autonomous mobile robotics applications. By measuring the round trip time of emitted light to and from objects in the scene, the system is capable of simultaneous full-field range imaging. This is achieved by projecting amplitude modulated continuous wave (AMCW) light onto the scene, and recording the reflection using an image sensor array with a high-speed shutter amplitude modulated at the same frequency (of the order of tens of MHz). The effect is to encode the phase delay of the reflected light as a change in pixel intensity, which is then interpreted as distance. A full field range imaging system has been constructed based on the PMD Technologies PMD19k image sensor, where the high-speed shuttering mechanism is builtin to the integrated circuit. This produces a system that is considerably more compact and power efficient than previous iterations that employed an image intensifier to provide sensor modulation. The new system has comparable performance to commercially available systems in terms of distance measurement precision and accuracy, but is much more flexible with regards to its operating parameters. All of the operating parameters, including the image integration time, sensor modulation phase offset and modulation frequency can be changed in realtime either manually or automatically through software. This highly configurable system serves as an excellent platform for research into novel range imaging techniques. One promising technique is the utilisation of measurements using multiple modulation frequencies in order to maximise precision over an extended operating range. Each measurement gives an independent estimate of the distance with limited range depending on the modulation frequency. These are combined to give a measurement with extended maximum range using a novel algorithm based on the New Chinese Remainder Theorem. A theoretical model for the measurement precision and accuracy of the new algorithm is presented and verified with experimental results. All distance image processing is performed on a per-pixel basis in real-time using a Field Programmable Gate Array (FPGA). An efficient hardware implementation of the phase determination algorithm for calculating distance is investigated. The limiting resource for such an implementation is random access memory (RAM), and a detailed analysis of the trade-off between this resource and measurement precision is also presented.</p>

Utilizing Schlieren Imaging to Visualize Heat Transfer Studies

2014 ◽  
Author(s):  
Jacob C. Kaessinger ◽  
Kramer C. Kors ◽  
Jordan S. Lum ◽  
Heather E. Dillon ◽  
Shannon K. Mayer
Keyword(s):  
Heat Transfer ◽  
Undergraduate Students ◽  
Cfd Simulation ◽  
Stokes Equations ◽  
Imaging System ◽  
Empirical Science ◽  
Navier Stokes ◽  
Schlieren Imaging ◽  
Navier Stokes Equations ◽  
Imaging Device

Convective heat transfer beyond explicit solutions to the Navier Stokes equations is often an empirical science. Schlieren imaging is one of the only fluid imaging systems that can directly visualize the density gradients of a fluid using collimated light and refractive properties. The ability to visualize fluid densities is useful in both research and educational fields. A Schlieren imaging device has been constructed by undergraduate students at the University of Portland. The device is used for professorial heat transfer and fluid dynamics research and to help undergraduates visualize and understand natural convection. This paper documents the design decisions, design process, and the final specifications of the Schlieren system. A simple 2-D heated cylindrical model is considered and evaluated using Schlieren imaging, OpenFOAM C.F.D. simulation, and convection analysis using a Nusselt correlation. Results are presented for the three analysis techniques and show excellent verifications between the CFD simulation, Nusselt correlation, and Schlieren imaging system.

Single-photon imaging system with a fiber optic taper

2018 ◽  
Vol 14 (4) ◽  
pp. 267-270
Author(s):  
Tian-xiang Zheng ◽  
Guang-yue Shen ◽  
Zhao-hui Li ◽  
E. Wu ◽  
Xiu-liang Chen ◽  
...  
Keyword(s):  
Fiber Optic ◽  
Imaging System ◽  
Single Photon ◽  
Photon Imaging

Compact dual band/dual FOV infrared imaging system with freeform prism

2021 ◽  
Vol 46 (4) ◽  
pp. 829
Author(s):  
Jun Yu ◽  
Zhengxiang Shen ◽  
Zhanshan Wang
Keyword(s):  
Infrared Imaging ◽  
Imaging System ◽  
Dual Band ◽  
Infrared Imaging System

scholarly journals Fabrication of a CMOS-based Imaging Chip with Monolithically Integrated RGB and NIR Filters

Proceedings ◽  
2019 ◽  
Vol 2 (13) ◽  
pp. 751
Author(s):  
Bart Vereecke ◽  
Els Van Besien ◽  
Deniz Sabuncuoglu Tezcan ◽  
Nick Spooren ◽  
Nicolaas Tack ◽  
...  
Keyword(s):  
Bandpass Filter ◽  
Imaging System ◽  
Low Cost ◽  
Cmos Technology ◽  
Image Sensors ◽  
Single Chip ◽  
Interference Filters ◽  
Monolithically Integrated ◽  
Recent Developments

Recent developments in multispectral cameras have demonstrated how compact and low-cost spectral sensors can be made by monolithically integrating filters on top of commercially available image sensors. In this paper, the fabrication of a RGB + NIR variation to such a single-chip imaging system is described, including the integration of a metallic shield to minimize crosstalk, and two interference filters: a NIR blocking filter, and a NIR bandpass filter. This is then combined with standard polymer based RGB colour filters. Fabrication of this chip is done in imec’s 200 mm cleanroom using standard CMOS technology, except for the addition of RGB colour filters and microlenses, which is outsourced.

Imaging Spectroscopy Using Fiber Optics

1997 ◽  
Vol 3 (S2) ◽  
pp. 845-846
Author(s):  
S. Michael Angel ◽  
H. Trey Skinner ◽  
Brian J. Marquardt
Keyword(s):  
Optical Fiber ◽  
Fiber Optics ◽  
Optical Fibers ◽  
Fiber Optic ◽  
Imaging System ◽  
Single Point ◽  
Imaging Spectroscopy ◽  
Small Diameter ◽  
Imaging Spectrometer ◽  
Remote Spectroscopy

Optical fiber probes are routinely used with optical spectrometers to allow measurements to be made on remotely located samples. In most of these systems, however, the optical fibers are used as non-imaging “light pipes” for the transmission of laser light, and luminescence or Raman signals to and from the sample. Thus, while these systems are suitable for remote spectroscopy, they are limited to single-point measurements. In a recent paper, we showed that a small-diameter (i.e., 350 μm) coherent optical fiber bundle can be combined with an AOTF-based imaging spectrometer for fluorescence and Raman spectral micro-imaging with increased flexibility in terms of sample positioning and in-situ capabilities. The previous paper described the operation of the fiber-optic microimaging probe and AOTF imaging system and showed preliminary Raman and fluorescence images for model compounds with 4 μm resolution. We have extended this work to include a discussion of the lateral and vertical spatial resolution of the fiber-optic microprobe in a non-contact proximity-focused configuration.

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