Pembersihan Isi Sel Akar dan Jenis Warna Tinta untuk Deteksi Cendawan Mikoriza Arbuskula

Arbuscular mycorrhizal (AM) fungi form mutualistic symbiosis with root of host plant. Staining technique to detect AM fungi usually used hazardous chemical. The ink stain and vinegar were used as an alternative technique to replace trypan blue and lactic acid in root staining method. This study aimed to determine time for clearing root cell contents and ink stain type to visualize the best AM fungal structures within the root observed under light microscope. Pueraria phaseoloides var. javanica roots colonized by AM fungi were cut into 1 cm long, cleared in KOH solution and stained. Four clearing time were done vis 5, 10, 15 and 20 minutes, and four stains were used namely Shaeffer black ink, Parker Quink blue ink, blue stamp ink, and trypan blue. Twenty stained roots were taken randomly from each tratment, and observed. Root clearing process for 20 minutes showed the best result. Only Shaeffer black ink and trypan blue produced clear structure of external hyphae, internal hyphae, vesicles and arbuscules. Arbuscular structure stained only by Shaeffer black ink and trypan blue. This indicated that Shaeffer black ink could be used as an alternative stain to detect AM fungi within the root of host plant

Effects of Funneliformis mosseae on the utilization of organic phosphorus in Camellia oleifera Abel

Arbuscular mycorrhizal (AM) fungi play an important role in the acquisition of phosphorus (P) by plants. The external hyphae of AM fungi function as an extension of plant roots and may downregulate related functions in the roots. It is not clear whether the ability of AM fungi to mineralize organic P affects root phosphatase activities. A pot experiment was conducted to investigate the effect of Funneliformis mosseae on soil organic P mineralization under phytate application and to explore root phosphatase activities, P uptake, and growth in Camellia oleifera Abel. The plants and their growth substrates were harvested 4 and 8 months after planting. The results showed that organic P application had no effect on the total dry mass of nonmycorrhizal plants, but differences in dry mass under P application were observed in mycorrhizal plants in both harvests. Inoculation with F. mosseae increased soil acid phosphatase, phytase, and alkaline phosphatase activities and reduced the soil organic P content. Mycorrhizal plants had higher root activity, shoot and root P contents and root acid phosphatase and phytase activities than nonmycorrhizal plants irrespective of organic P application. In conclusion, AM fungi enhanced the mineralization of soil organic P and positively affect root phosphatase activities.

Arbusculer mycorrizal fungal investigation in the weeds of wheat field of Tehsil Tangi, District Charsadda, Khyber Pakhtunkhwa.

An investigation has been make about the Arbuscular Mycorrhizal Fungi (AMF) spore density in weeds plants with soil selected from the Tehsil Tangi. The spore density was observed in all weeds plants in the host growth stage. The maximum Arbuscular Mycorrhizal Fungi (AMF) spore density wasfound in Chenopodium album (93.00±74.02) and Scandix iberica (89.00±92.08) at vegetative stage. The Glomus species are dominant in the rhizosporic soil of weeds plants followed in Aculospora, and Sclerocystis. The lowest Glomus spore density are present in Fumariapureflora (10.67±15.89).The spore density of AMF had a strong positive correlation with soil PH and carbon is negative correlation with P content of the soil. Thehighest Vesicles are investigated in.Euphorbia heliscopia(14.33±9.5) and lowest in Circium arevesis (3.33±1.53) .Arbuscules was higher in Euphorbia heliscopia (18.67±18.72) and lowest in Circium arvensis (2.00±1.73). Highest External hyphae was found in Silene conidia (4.00±1.73) and lowest in Anagallus arrensis. Internal hyphae were in Rananculas maricates (6.33±5.13) and lowest in Melolotus indica(0.67±0.58). While totally absent in Brassica nigra (Brassicaece).

Keragaman Vegetasi dan Identifikasi Fungi Mikoriza Arbuskula (FMA) Pada Lahan Bekas Tambang

Revegetation of former gold mining land is expected to restore soil fertility. One of the ways to use the gold-mined land as agricultural land is by utilizing the symbioses of arbuscular mycorrhizal fungi (AMF) is expected to accelerate the revegetation process. The aim of the study was to analyze vegetation and AMF exploration in secondary forest land (SF) and former gold mining land (FGML) in the Mandor District . The research stages were conducting the plant identification and the exploration of AMF at the laboratory of Pests and Diseases of Faculty of Agriculture, University of Tanjungpura. The research was conducted for ± 2 months starting from February to April 2018. The parameter of the observation was done by finding the summed dominance ratio (SDR) value, diversity index (DI), the density of spore (DS), relative frequency (RF), the identification of spore, and the analysis of plant root infection. The research finding showed that from the calculation of SDR, there were two dominant plants i.e. Paspalum conjugatum and Melastoma candidum. The calculation result of DI was categorized as ‘moderate’ in three locations of observation. The observation of DS in SF obtained the highest value of 750 spores/100 grams of soil. The total genus Glomus in SF and FGML > 10 years had a higher value than genus Acaulospora. In the three locations of observation, it had been obtained the genus Glomus and Acaulospora, while there was an infection at the observation of staining roots; it was characterized by the existence of arbuscular, vesicles, and external hyphae.

Coordinated Changes In The Accumulation Of Metal Ions In Maize (Zea mays ssp. mays L.) In Response To Inoculation With The Arbuscular Mycorrhizal Fungus Funneliformis mosseae

Arbuscular mycorrhizal symbiosis is an ancient interaction between plants and fungi of the phylum Glomeromycota. In exchange for photosynthetically fixed carbon, the fungus provides the plant host with greater access to soil nutrients via an extensive network of root-external hyphae. Here, to determine the impact of the symbiosis on the host ionome, the concentration of nineteen elements was determined in the roots and leaves of a panel of thirty maize varieties, grown under phosphorus limiting conditions, with, or without, inoculation with the fungus Funneliformis mosseae. Although the most recognized benefit of the symbiosis to the host plant is greater access to soil phosphorus, the concentration of a number of other elements responded significantly to inoculation across the panel as a whole. In addition, variety-specific effects indicated the importance of plant genotype to the response. Clusters of elements were identified that varied in a coordinated manner across genotypes, and that were maintained between non-inoculated and inoculated plants.AbbreviationsNCnon-colonizedMmycorrhizalSDWshoot dry weightICP-MSinductively coupled plasma mass spectrometryPCprincipal component