scholarly journals Pembersihan Isi Sel Akar dan Jenis Warna Tinta untuk Deteksi Cendawan Mikoriza Arbuskula

2021 ◽  
Vol 7 (1) ◽  
pp. 36-40
Author(s):  
Siti Sulfiah ◽  
Nampiah Sukarno ◽  
Agustin Wydia Gunawan
Keyword(s):  
Host Plant ◽  
Trypan Blue ◽  
Staining Method ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Staining Technique ◽  
Mutualistic Symbiosis ◽  
Hazardous Chemical ◽  
External Hyphae ◽  
Clearing Time

Arbuscular mycorrhizal (AM) fungi form mutualistic symbiosis with root of host plant. Staining technique to detect AM fungi usually used hazardous chemical. The ink stain and vinegar were used as an alternative technique to replace trypan blue and lactic acid in root staining method. This study aimed to determine time for clearing root cell contents and ink stain type to visualize the best AM fungal structures within the root observed under light microscope. Pueraria phaseoloides var. javanica roots colonized by AM fungi were cut into 1 cm long, cleared in KOH solution and stained.  Four clearing time were done vis 5, 10, 15 and 20 minutes, and four stains were used namely Shaeffer black ink, Parker Quink blue ink, blue stamp ink, and trypan blue. Twenty stained roots were taken randomly from each tratment, and observed. Root clearing process for 20 minutes showed the best result. Only Shaeffer black ink and trypan blue produced clear structure of external hyphae, internal hyphae, vesicles and arbuscules. Arbuscular structure stained only by Shaeffer black ink and trypan blue. This indicated that Shaeffer black ink could be used as an alternative stain to detect AM fungi within the root of host plant

scholarly journals Haustorium Formation in Medicago truncatula Roots Infected by Phytophthora palmivora Does Not Involve the Common Endosymbiotic Program Shared by Arbuscular Mycorrhizal Fungi and Rhizobia

2015 ◽  
Vol 28 (12) ◽  
pp. 1271-1280 ◽  
Author(s):  
Rik Huisman ◽  
Klaas Bouwmeester ◽  
Marijke Brattinga ◽  
Francine Govers ◽  
Ton Bisseling ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Phytophthora Palmivora ◽  
Living Plant ◽  
Mutualistic Symbiosis ◽  
Membrane Compartment ◽  
Microbe Interactions ◽  
Host Genes

In biotrophic plant-microbe interactions, microbes infect living plant cells, in which they are hosted in a novel membrane compartment, the host-microbe interface. To create a host-microbe interface, arbuscular mycorrhizal (AM) fungi and rhizobia make use of the same endosymbiotic program. It is a long-standing hypothesis that pathogens make use of plant proteins that are dedicated to mutualistic symbiosis to infect plants and form haustoria. In this report, we developed a Phytophthora palmivora pathosystem to study haustorium formation in Medicago truncatula roots. We show that P. palmivora does not require host genes that are essential for symbiotic infection and host-microbe interface formation to infect Medicago roots and form haustoria. Based on these findings, we conclude that P. palmivora does not hijack the ancient intracellular accommodation program used by symbiotic microbes to form a biotrophic host-microbe interface.

scholarly journals Species-Specific Interactions of Bacillus Innocula and Arbuscular Mycorrhizal Fungi Symbiosis with Winter Wheat

2020 ◽  
Vol 8 (11) ◽  
pp. 1795
Author(s):  
Thomas I. Wilkes ◽  
Douglas J. Warner ◽  
Veronica Edmonds-Brown ◽  
Keith G. Davies
Keyword(s):  
Bacillus Subtilis ◽  
Winter Wheat ◽  
Host Plant ◽  
Root Length ◽  
Vascular Plant ◽  
Fungal Growth ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Fungal Symbiosis ◽  
Species Specific

Arbuscular mycorrhizal (AM) fungi establish close interactions with host plants, an estimated 80% of vascular plant species. The host plant receives additional soil bound nutrients that would otherwise not be available. Other components of the microbiome, such as rhizobacteria, may influence interactions between AM fungi and the host plant. Within a commercial arable crop selected rhizobacteria in combination with AM fungi may benefit crop yields. The precise nature of interactions between rhizobacteria and AM fungi in a symbiotic relationship overall requires greater understanding. The present study aims to assess this relationship by quantifying: (1) AM fungal intracellular root structures (arbuscules) and soil glomalin as an indicator of AM fungal growth; and (2) root length and tiller number as a measure of crop growth, in response to inoculation with one of three species of Bacillus: B. amyloliquefaciences, B. pumilis, or B. subtilis. The influence of soil management, conventional (CT) or zero tillage (ZT) was a further variable evaluated. A significant (p < 0.0001) species-specific impact on the number of quantifiable AM fungal arbuscules was observed. The inoculation of winter wheat (Triticum aestivum) with B. amyloliquefaciences had a positive impact on AM fungal symbiosis, as indicated by an average of 3226 arbuscules per centimetre of root tissue. Bacillus subtilis increased root length significantly (p < 0.01) but decreased fungal symbiosis (p < 0.01). The inoculation of field soils altered the concentration of glomalin, an indicator of AM fungal growth, significantly (p < 0.00001) for each tillage treatment. The greatest increase was associated with B. amyloliquefaciences for both CT (p < 0.0001) and ZT (p < 0.00001). Bacillus subtilis reduced measured glomalin significantly in both tillage treatments (p < 0.0001 and p < 0.00001 for CT and ZT respectively). The interaction between rhizobacteria and AM fungi is variable, being beneficial or detrimental depending on species. This relationship was evident in both tillage treatments and has important implications for maximizing symbiosis in the crop plant-microbiome present in agricultural systems.

Novel Insights into Host Receptors and Receptor-mediated Signaling that Regulate Arbuscular Mycorrhizal Symbiosis

2020 ◽  
Author(s):  
Fahad Nasir ◽  
Ali Bahadur ◽  
Xiaolong Lin ◽  
Yingzhi Gao ◽  
Chunjie Tian
Keyword(s):  
Host Plant ◽  
Host Plants ◽  
Agronomic Traits ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Land Plant ◽  
Physical Contact ◽  
Mycorrhizal Symbiosis ◽  
Downstream Signaling ◽  
Am Symbiosis

Abstract More than 80% of land plant species benefit from symbiotic partnerships with arbuscular mycorrhizal (AM) fungi that assist in nutrient acquisition and enhance the ability of host plants to adapt to environmental constraints. Host-generated plasma membrane-residing receptor-like kinases and the α/β-hydrolases, e.g. DWARF14-LIKE (D14L), a putative karrikin receptor, are used to detect the presence of AM fungi prior to physical contact between the host and fungus. Detection induces the activation of symbiosis-related transcriptional programming, enabling the successful establishment of AM symbiosis. In order to prevent hyper-colonization and to maintain a mutually beneficial association, the host plants precisely monitor and control AM symbiosis during the post-symbiotic stage via different molecular strategies. While previous studies have elucidated how host plant receptors and receptor-mediated signaling regulate AM symbiosis, the molecular details underlying these processes remain poorly understood. The recent identification of a rice (Oryza sativa) CHITIN-ELICITOR RECEPTOR-KINASE 1 (OsCERK1) interaction partner MYC FACTOR RECEPTOR 1 (OsMYR1), as well as new insights into D14L-receptor- and SUPER NUMERIC NODULES 1 (SUNN1) receptor-mediated signaling have improved our understanding of how host plant receptors and their corresponding signaling regulate AM symbiosis. The present review summarizes these and other current findings that have increased our limited understanding of receptor-mediated signaling mechanisms involved in the regulation of AM symbiosis. The identified receptors and/or their downstream signaling components could potentially be used to engineer economically-important crops with improved agronomic traits by conferring the ability to control the colonization of AM fungi in a precise manner.

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Hydrologic Gradients in the Rhizosphere ofPhragmites australis(Cav.) Trin ex. Steudel Growing in the Sun Island Wetland

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Li Wang ◽  
Jieting Wu ◽  
Fang Ma ◽  
Jixian Yang ◽  
Shiyang Li ◽  
...  
Keyword(s):  
Host Plant ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
The Sun ◽  
Vegetative Period ◽  
Hydrologic Gradients ◽  
Mycorrhizal Association

Within the rhizosphere, AM fungi are a sensitive variable to changes of botanic and environmental conditions, and they may interact with the biomass of plant and other microbes. During the vegetative period of thePhragmites australisgrowing in the Sun Island Wetland (SIW), the variations of AM fungi colonization were studied. Root samples of three hydrologic gradients generally showed AM fungi colonization, suggesting that AM fungi have the ability for adaptation to flooded habitats. There were direct and indirect hydrological related effects with respect to AM fungi biomass, which interacted simultaneously in the rhizosphere. Though water content in soil and reed growth parameters were both positively associated with AM fungi colonization, only the positive correlations between reed biomass parameters and the colonization could be expected, or both the host plant biomass and the AM fungi could be beneficial. The variations in response of host plant to the edaphic and hydrologic conditions may influence the effectiveness of the plant-mycorrhizal association. This study included a hydrologic component to better assess the role and distribution of AM fungi in wetland ecosystems. And because of that, the range of AM fungi was extended, since they actually showed a notable adaptability to hydrologic gradients.

scholarly journals Ink and Vinegar, a Simple Staining Technique for Arbuscular-Mycorrhizal Fungi

1998 ◽  
Vol 64 (12) ◽  
pp. 5004-5007 ◽  
Author(s):  
Horst Vierheilig ◽  
Andrew P. Coughlan ◽  
Urs Wyss ◽  
Yves Piché
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Staining Method ◽  
Arbuscular Mycorrhizal ◽  
Staining Technique ◽  
High Quality ◽  
Simple Method ◽  
Great Utility ◽  
Root Tissues ◽  
Arbuscular Mycorrhizal Fungal

ABSTRACT We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.

scholarly journals Alternative and safe dyes for staining Arbuscular Mycorrhizal fungi

2020 ◽  
Vol 11 (7) ◽  
pp. 400-408
Author(s):  
Marcos Diones Ferreira Santana ◽  
Túlio Silva Lara ◽  
Sheyla Regina Marques Couceiro
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Trypan Blue ◽  
Organic Dyes ◽  
Low Cost ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Euterpe Oleracea ◽  
Study And Teaching ◽  
Euterpe Oleracea Mart

Several methodologies are available for the study of Arbuscular Mycorrhizal fungi (AM). Most involving the colouring of typical symbiosis structures, with the use of dyes such as Trypan Blue, which can be harmful to users' health (e.g., carcinogens) and the environment (e.g., pollutants). In order to use safe alternatives for AM fungi studies, a comparison and analysis of the use of industrial organic food dyes (Arcólor®) in blue and red colours, organic dye based on Euterpe oleracea Mart. pulp (açaí) and the traditional Trypan Blue (control), all of them diluted in commercial vinegar, for observation and quantification of the typical structures of mycorrhizal infection in the roots of Vigna unguiculata L., Schizolobium amazonicum Huber ex Ducke, Zea mays L. and Brachiaria sp. The results indicated that the organic dyes visually colour the structures of the AM fungi. However, blue food colouring is as effective as Trypan Blue, in addition to being a good option in teaching activities evolving AM fungi, mainly because it presents less risk to users' health. Thus, a good substitute for the Trypan Blue synthetic dye is presented, based on an effective, easily applicable, low cost and safe methodology for the study and teaching of AM fungi.

scholarly journals Succession in arbuscular mycorrhizal fungi can be attributed to a chronosequence of Cunninghamia lanceolata

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nini Lu ◽  
Xuelei Xu ◽  
Ping Wang ◽  
Peng Zhang ◽  
Baoming Ji ◽  
...  
Keyword(s):  
Host Plant ◽  
Fungal Community ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Chinese Fir ◽  
Fungal Communities ◽  
Available P ◽  
Total N ◽  
Available N ◽  
Significant Difference

AbstractArbuscular mycorrhizal (AM) fungi play an important role in plant-fungi communities. It remains a central question of how the AM fungal community changes as plants grow. To establish an understanding of AM fungal community dynamics associated with Chinese fir, Chinese fir with five different growth stages were studied and 60 root samples were collected at the Jiangle National Forestry Farm, Fujian Province. A total of 76 AM fungal operational taxonomic units (OTUs) were identified by high-throughput sequencing on an Illumina Miseq platform. The genera covered by OTUs were Glomus, Archaeospora, Acaulospora, Gigaspora and Diversispora. Glomus dominated the community in the whole stage. The number and composition of OTUs varied along with the host plant growth. The number of OTUs showed an inverted V-shaped change with the host plant age, and the maximum occurred in 23-year. Overall, the basic species diversity and richness in this study were stable. Non-metric multi-dimensional scaling (NMDS) analysis based on bray-curtis distance revealed that there were remarkable differentiations between the 9-year and other stages. Besides, AM fungal community in 32-year had a significant difference with that of 23-year, while no significant difference with that of 45-year, suggesting that 32-year may be a steady stage for AM fungi associated with Chinese fir. The cutting age in 32-year may be the most favorable for microbial community. The pH, total N, total P, total K, available N, available P, available K, organic matter and Mg varied as the Chinese fir grows. According to Mantel test and redundancy analysis, available N, available P, K and Mg could exert significant influence on AM fungal communities, and these variables explained 31% of variance in the composition of AM fungal communities.

scholarly journals OsRAM2 function in lipid biosynthesis is required for arbuscular mycorrhizal symbiosis in rice

2021 ◽  
Author(s):  
Ying-Na Liu ◽  
Cheng-Chen Liu ◽  
An-Qi Zhu ◽  
Ke-Xin Niu ◽  
Rui Guo ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Root Surface ◽  
Mycorrhizal Symbiosis ◽  
Cortical Cells ◽  
Mutualistic Symbiosis ◽  
Fungal Hyphae ◽  
Mutant Lines

Arbuscular mycorrhiza (AM) is a mutualistic symbiosis formed between most land plants and Glomeromycotina fungi. During the symbiosis, plants provide organic carbon to fungi in exchange for mineral nutrients. Previous legume studies showed that the Required for Arbuscular Mycorrhization2 (RAM2) gene is necessary for transferring lipids from plants to AM fungi (AMF) and is also likely to play a ‘signaling’ role at the root surface. To further explore RAM2 functions in other plant lineages, in this study, two rice (Oryza sativa) genes, OsRAM2 and OsRAM2L, were identified as orthologs of legume RAM2. Examining their expression patterns during symbiosis revealed that only OsRAM2 was strongly upregulated upon AMF inoculation. CRISPR/Cas9 mutagenesis was then performed to obtain three Osram2 mutant lines (-1, -2, and -3). After inoculation by AMF Rhizophagus irregularis or Funneliformis mosseae, all the mutant lines showed extremely low colonization rates and the rarely observed arbuscules were all defective, thus supporting a conserved ‘nutritional’ role of RAM2 between monocot and dicot lineages. As for the ‘signaling’ role, although the hyphopodia numbers formed by both AMF on Osram2 mutants were indeed reduced, their morphology showed no abnormality, with fungal hyphae invading roots successfully. Promoter activities further indicated OsRAM2 was not expressed in epidermal cells below hyphopodia or outer cortical cells enclosing fungal hyphae, but expressed exclusively in cortical cells containing arbuscules. It therefore suggested an indirect role of RAM2 rather than a direct involvement in determining the symbiosis signals at the root surface.

scholarly journals Hormonomic Changes Driving the Negative Impact of Broomrape on Plant Host Interactions with Arbuscular Mycorrhizal Fungi

2021 ◽  
Vol 22 (24) ◽  
pp. 13677
Author(s):  
Kiril Mishev ◽  
Petre I. Dobrev ◽  
Jozef Lacek ◽  
Roberta Filepová ◽  
Bistra Yuperlieva-Mateeva ◽  
...  
Keyword(s):  
Host Plant ◽  
Mycorrhizal Fungi ◽  
Negative Impact ◽  
Dominant Role ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Parasitic Plants ◽  
Plant Roots ◽  
Plant Host ◽  
Mycorrhizal Roots

Belowground interactions of plants with other organisms in the rhizosphere rely on extensive small-molecule communication. Chemical signals released from host plant roots ensure the development of beneficial arbuscular mycorrhizal (AM) fungi which in turn modulate host plant growth and stress tolerance. However, parasitic plants have adopted the capacity to sense the same signaling molecules and to trigger their own seed germination in the immediate vicinity of host roots. The contribution of AM fungi and parasitic plants to the regulation of phytohormone levels in host plant roots and root exudates remains largely obscure. Here, we studied the hormonome in the model system comprising tobacco as a host plant, Phelipanche spp. as a holoparasitic plant, and the AM fungus Rhizophagus irregularis. Co-cultivation of tobacco with broomrape and AM fungi alone or in combination led to characteristic changes in the levels of endogenous and exuded abscisic acid, indole-3-acetic acid, cytokinins, salicylic acid, and orobanchol-type strigolactones. The hormonal content in exudates of broomrape-infested mycorrhizal roots resembled that in exudates of infested non-mycorrhizal roots and differed from that observed in exudates of non-infested mycorrhizal roots. Moreover, we observed a significant reduction in AM colonization of infested tobacco plants, pointing to a dominant role of the holoparasite within the tripartite system.

scholarly journals Arbuscular mycorrhiza in species of Commelinidae (Liliopsida) in the state of Pernambuco (Brazil)

2001 ◽  
Vol 15 (2) ◽  
pp. 155-165 ◽  
Author(s):  
Gladstone Alves da Silva ◽  
Bartolomeu Acioli dos Santos ◽  
Marccus Vinícius Alves ◽  
Leonor Costa Maia
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Light Microscope ◽  
Trypan Blue ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
The State ◽  
Plant Roots ◽  
Mutualistic Symbiosis ◽  
Important Kind

Mycorrhiza are a mutualistic symbiosis between fungi and plant roots, the main benefit to the plant being increased nutrient uptake. The arbuscular is the most important kind of mycorrhiza for agriculture and it is widespread in occurrence and distribution in most ecosystems. The aim of this work was to study the mycorrhizal status of the species of Commelinidae that occur in the State of Pernambuco. Plant roots, collected in ten municipalities, were washed, cleared in KOH, stained with Trypan blue in lactoglycerol and observed under a light microscope in order to assess presence and identification of the mycorrhizal type. Percentage of root colonization was evaluated by the gridline intersect method. Forty specimens representing 30 species were observed. From these specimens, 70% were colonized by arbuscular mycorrhizal fungi (AMF). In one family (Typhaceae), mycorrhizal structures were not observed, in two of them (Eriocaulaceae and Juncaceae) all specimens showed the association, and three families (Commelinaceae, Cyperaceae and Poaceae) presented specimens with or without AMF. In some of the roots, other fungi were observed together with the AMF. The results indicate that AMF are widely distributed among species of Commelinidae in Pernambuco, being probably important for their establishment in the areas visited.

Sign in / Sign up

Export Citation Format

Share Document