In wayside fault diagnosis of train bearings, the phenomenon of Doppler distortion in the acoustic signal of moving acoustic source acquired with a microphone leads to the difficulty for signal analysis. In this paper, a new method based on Dopplerlet transform and re-sampling is proposed to remove the Doppler distortion, and applied in the fault diagnosis of train bearings. Firstly, search the parameters space to find the primary functions-Dopplerlet atoms. According to the Morse acoustic theory and Doppler effect, the instantaneous frequency of the Dopplerlet atom which we choose to remove Doppler distortion of the corresponding acoustic source can be acquired. Then, the re-sampling sequence can be established as the re-sampling vector in time domain. Through the resample, the Doppler distortion effect can be removed. Finally, simulations and experiments with practical acoustic signals of train bearings with a defect on the outer race and the inner race are carried out, and the results verified the effectiveness of this method. Comparing with the other methods of Doppler distortion removal, this method works without measuring the motion parameters in advance, and is quite robust to noise. Meanwhile, this method has the potential to eliminate the Doppler distortion of original signal with multiple sources.