Rediscovery of the rare Phrynomedusa appendiculata (Lutz, 1925) (Anura: Phyllomedusidae) from the Atlantic Forest of southeastern Brazil

The genus Phrynomedusa Miranda-Ribeiro, 1923 comprises rare and little known phyllomedusid species from southern Atlantic Forest, Brazil. Phrynomedusa appendiculata (Lutz, 1925) is known from three localities since its description and considered a “lost species” because it was last sighted 51 years ago. This pervasive lack of knowledge raised a significant concern about its threat status. Here, we present the rediscovery of P. appendiculata from a breeding population in the Atlantic Plateau forests of the state of São Paulo. This new record allowed the gathering of novel ecological, acoustic and morphological data for this species. Most of the novel data agreed with the variation historically reported for the species, but we found subtle divergences that we interpret as intraspecific variation. Moreover, this record also allowed a reassessment of geographic distribution of the species, and the first inference of its phylogenetic relationships based on molecular data (mitochondrial and nuclear DNA). The resulting phylogeny corroborated the generic placement and evolutionary distinctiveness of P. appendiculata, evidencing the species as sister to the clade P. marginata + P. dryade. Based on novel and historical data, we discuss some putative factors influencing the rarity of P. appendiculata and its congeners, and provide conservation perspectives. We expect that the novel data can support further assessments of threat status for this rare species, as well as initiatives aiming its conservation.

Conserving evolutionarily distinct species is critical to safeguard human well-being

Although there is growing interest in safeguarding the Tree of Life to preserve the human benefits that are directly provided by biodiversity, their evolutionary distribution remains unknown, which has hampered our understanding of the potential of phylodiversity indicators to evince them. Here, I drew on a global review of plant benefits and comprehensive phylogenetic information to breakdown their evolutionary distribution and thereby show why the commonly used Phylogenetic Diversity and Evolutionary Distinctiveness indicators can unequivocally help to preserve these natural services. Beneficial species clumped within phylogenetically overdispersed genera and closely related species often contributed very few and redundant benefits, suggesting that multiple plant lineages are required to maintain a wide variety of services. Yet, a reduced number of species stood out as multi-beneficial and evolutionarily distinct plants relative to both the entire phylogeny and the subset of beneficial species, and they collectively contributed a higher-than-expected number of records for most types of benefits. In addition to providing a clear mechanistic understanding for the recently proved success of Phylogenetic Diversity in capturing plant benefits, these findings stress the decisive role that conservation programmes aimed at protecting evolutionarily distinct taxa will play in safeguarding the beneficial potential of biodiversity for the future.

Molecular phylogeny reveals a new genus of freshwater mussels from the Mekong River Basin (Bivalvia: Unionidae)

A new genus of freshwater mussels (Bivalvia: Unionidae) is described from the Mekong River Basin as Namkongnaia gen. nov. The validity of the new genus is supported by its unique conchological characteristics, namely the lack of hinge dentition and elongated shells, together with its evolutionary distinctiveness as estimated by multi-locus phylogenetic analyses (mitochondrial COI and 16S, and nuclear 28S genes). The new genus includes two lineages with deep divergence, shown by 5.10% uncorrected COI p-distance. One lineage is a type species described herein as Namkongnaia inkhavilayi gen. et sp. nov. The other is a recognized species under the name ‘Pilsbryoconcha lemeslei (Morelet, 1875)’. Molecular phylogenetic analysis further shows that the new genus belongs to the tribe Pseudodontini, and evolutionarily is closely related to the genus Monodontina Conrad, 1853. However, its conchology is similar to the genus Pilsbryoconcha Simpson, 1900. Time-calibrated phylogeny suggests that the main radiation events of the tribe Pseudodontini occurred during the Late Cretaceous to the Eocene, with the divergence between the new genus and Monodontina placed in the Miocene. The discovery of new freshwater mussel taxa in this study highlights the importance of the Mekong River Basin as one of the world’s biodiversity hotspots for freshwater fauna.

Specialist Bee Species Are Larger and Less Phylogenetically Distinct Than Generalists in Tropical Plant–Bee Interaction Networks

Bee pollinators are key components of terrestrial ecosystems. Evidence is mounting that bees are globally in decline, and species with a higher degree of specialization are the most vulnerable to local extinction. However, ecological features that could explain bee specialization remain poorly tested, especially in tropical species. Here, we aim to determine the most specialized bee species and their associated ecological traits in tropical plant–bee interaction networks, answering three questions: (1) Which bees in the interaction networks are specialists? (2) Is body size related to their role as specialists in interaction networks? (3) Are there phylogenetic relationships between the bee species identified as specialists? We used fifteen quantitative plant–bee interaction networks from different Brazilian biomes covering 1,702 interactions (386 bee and 717 plant species). We used the normalized degree (standardized number of partners) as a metric to determine trophic specialization of bee species. Body size was estimated by measuring intertegular distance (ITD), i.e., the distance between the bases of the wings on the thorax. Evolutionary distinctiveness (ED) was used to quantify species uniqueness, i.e., the singularity of species in the phylogenetic tree. Relationships between dietary specialism, ITD and ED were assessed using generalized linear models. We detected 34 specialist bee species (9% of total species), distributed in 13 genera, and four families. ITD and ED were important variables explaining the specialization of tropical bee species. Specialists were larger and less phylogenetically distinct than expected by chance. Based on a large data set covering some of the main tropical biomes, our results suggest that loss of specialist bees from Brazilian plant–bee networks could have deleterious consequences for native plant species preferentially pollinated by large-bodied bees. Moreover, by affecting more evolutionarily distinct species, i.e., those with fewer extant relatives, the loss of specialist bees will likely affect few clades but can result on considerable loss of evolutionary history and phylogenetic diversity in the Brazilian bee communities. The results are important for decision-making concerning conservation measures for these species and may also encourage the development of sustainable management techniques for bees.

Conservation genetics of conflict in the Asian elephant, Elephas maximus

The Asian elephant (Elephas maximus) is an endangered species whose distribution spans 13 countries in south, southeast, and insular Asia. The primary threats to the survival of this species include direct conflict, primarily in the forms of poaching and crop-raiding, as well indirect conflict such as habitat loss and fragmentation. Due to the elephant's elusive behavior within their dense vegetative habitat and the fact that their large size presents handling dangers to researchers and elephants, research increasingly relies on noninvasive monitoring combined with a diverse assemblage of genetic tools. This dissertation uses conservation genetics to evaluate major conflict and conservation issues for Asian elephants. The first study, in the Bago Yoma region of Myanmar, evaluates the impact of direct human-conflict in a high-density area of humans and elephants. Here elephants are heavily impacted by the developing skin trade, and condensed populations frequently raid local farms forined the overall population structure and gained demographic insights as to what defines a crop-raider. The second study, conducted in the Nakai plateau elephant population of Lao PDR, compared and contrasted the diversity and demography of a population of high conservation value before and after the construction of a hydroelectric dam. The results revealed a seasonally shifting population, unique from the previous occupants of the plateau, and a decline in genetic diversity. In the final study, genetic data from across the species' range was used to identify hotspots of genetic diversity despite marker selection bias, a problem frequently encountered in conservation genetic studies. The results highlight the evolutionary distinctiveness and conservation value of populations, particularly in southeast Asia, for conservation management of this iconic species.

Conserving Evolutionarily Distinct Species is Critical to Safeguard Human Well-Being

Although there is growing interest in safeguarding the Tree of Life to preserve the human benefits that are directly provided by biodiversity, their evolutionary distribution remains unknown, which has hampered our understanding of the potential of phylodiversity indicators to evince them. Here, I drew on a global review of plant services and comprehensive phylogenetic information to breakdown their evolutionary distribution and thereby show why the commonly used Phylogenetic Diversity and Evolutionary Distinctiveness indicators can unequivocally help to preserve these natural benefits. Beneficial species clumped within phylogenetically overdispersed genera and closely related taxa often contributed very few and redundant services, suggesting that multiple plant lineages are required to maintain a wide variety of benefits. Yet, a reduced number of species stood out as multi-beneficial and evolutionarily distinct plants relative to both the entire phylogeny and the subset of beneficial species, and they collectively contributed a higher-than-expected number of records for most types of benefits. In addition to providing a clear mechanistic understanding for the recently proved success of Phylogenetic Diversity in capturing plant benefits, these findings stress the decisive role that conservation programmes aimed at protecting evolutionarily distinct taxa will pay in safeguarding the beneficial potential of biodiversity for the future.

Developing global vulnerabilities and conservation priorities for cave-dwelling bats

Research and conservation interventions are disproportionately focused on taxa perceived as charismatic, while other systems with high levels of endemism, and often under-protected such as caves and subterranean ecosystems remain neglected. Bats are keystone to cave ecosystems making them ideal surrogates to understand diversity patterns and assay conservation priorities. Using a novel framework, we assessed and mapped the global bat cave vulnerabilities and priorities at the biome and site level. Almost half (N = 678, 48%) of bat species across the world regularly use caves, with 32% endemic to a single country, and 15% currently threatened with extinction. Tropical realms and small-ranged species faced the highest levels of risk. Our analyses consistently showed that most caves with lower threat levels show higher cave biotic potential (i.e., evolutionary distinctiveness and endemism), though this may be due to the loss of species from more disturbed caves. We estimate that 3% to 28% are high-priority caves for conservation in broad-scale (biome level) and fine-scale (site-dependent) analyses respectively. Amongst regions, the highest concentration of conservation priority areas are in the Palearctic and tropical regions (except Afrotropical), which requires more intensive data sampling. Our results further highlight the importance of prioritising bat caves at local scales but show that broader scale analysis is possible if robust cave data is present and effective parameters are included (i.e., appropriate landscape features and threats). Developing priorities for these systems requires more systematic approaches as a standard measure to develop the priorities needed to maintain cave diversity.

A global phylogenetic regionalisation of vascular plants reveals a deep split between Gondwanan and Laurasian biotas

Existing global regionalisation schemes for plants consider the compositional affinities among biotas, but these have not considered phylogenetic information explicitly. Incorporating phylogenetic information may substantially advance our understanding of the relationships among regions and the synopsis of biogeographical schemes, because phylogeny captures information on the evolutionary history of taxa. Here, we present the first phytogeographical delineation of the global vascular flora based on the evolutionary relationships of species. We analysed 8,738,520 geographical occurrence records for vascular plant species together with a time-calibrated phylogenetic tree including 67,420 species. We estimated species composition within 200x200 km grid cells across the world, and used a metric of phylogenetic beta diversity to generate a phylogenetic delineation of floristic regions. We contrasted these results with a regionalisation generated using a taxonomic beta diversity metric. We identified 16 phylogenetically distinct phytogeographical units, deeply split into two main clusters that broadly correspond to the Laurasia-Gondwana separation. Our regionalisation broadly matches currently recognized phytogeographical classifications, but also highlights that the Gondwanan area is split into a large Holotropical cluster and an Australian-NeoZelandic-Patagonian cluster. In turn, we found that the northernmost and southernmost units have the most evolutionarily distinct vascular plant assemblages. In contrast, taxonomic dissimilarity returned a regionalisation composed of 23 units with a high degree of shared taxa between Laurasian and Gondwanan areas, with no clear split among their biotas. The integration of phylogenetic information provided new insights into the historical relationships among phytogeographical units, enabling the identification of three large, clearly differentiated biotas, here referred to as kingdoms: Holarctic, Holotropical, and Austral. Our regionalization scheme provides further evidence for delineating transition zones between the Holarctic and Holotropical kingdoms. The latitudinal patterns of evolutionary distinctiveness of vascular plant assemblages are consistent with recent evidence of higher and more recent diversification of flowering plants outside tropical regions.

Integrating spatial, phylogenetic, and threat assessment data from frogs and lizards to identify areas for conservation priorities in Peninsular Malaysia

Malaysia is recognized as a megadiverse country and biodiversity hotspot, which necessitates sufficient levels of habitat protection and effective conservation management. However, conservation planning in Malaysia has hitherto relied largely on species distribution data without taking into account the rich evolutionary history of taxa. This represents the first study that integrates spatial and evolutionary approaches to identify important centers of diversity, endemism, and bioregionalization that can be earmarked for conservation priorities in Peninsular Malaysia. Using georeferenced species occurrences, comprehensive phylogenies, and threat assessments of frogs and lizards, we employed a spatial phylogenetics framework that incorporates various diversity metrics including weighted endemism, phylogenetic diversity, phylogenetic endemism, and evolutionary distinctiveness and global endangerment. Ten areas of high conservation value were identified via the intersection of these metrics, i.e., northern Perlis, Langkawi Geopark, southern Bintang range, Cameron Highlands, Frasers Hill, Benom-Krau complex, Selangor-Genting complex, Endau-Rompin National Park, Seribuat Archipelago (Tioman and Pemanggil Islands), and southern Johor. Of these, Cameron Highlands requires the highest conservation priority based on severe environmental degradation, inadequately protected areas, and high numbers of endangered and evolutionary distinct species. Other areas, especially in the northwestern (states of Kedah and Penang) and northeastern regions (states of Kelantan) were not only identified as areas of high conservation value but also areas of biogeographic importance. Taken together, frogs and lizards demonstrate distinct east-west and north-south patterns of bioregionalization that are largely modulated by mountain ranges.

Impediments to Understanding Seagrasses’ Response to Global Change

Uncertainties from sampling biases present challenges to ecologists and evolutionary biologists in understanding species sensitivity to anthropogenic climate change. Here, we synthesize possible impediments that can constrain research to assess present and future seagrass response from climate change. First, our knowledge of seagrass occurrence information is prevalent with biases, gaps and uncertainties that can influence inferences on species response to global change. Second, research on seagrass diversity has been focused on species-level metrics that can be measured with data from the present – but rarely accounting for the shared phylogenetic relationships and evolutionary distinctiveness of species despite species evolved and diversified from shared ancestors. Third, compared to the mass production of species occurrence records, computational tools that can analyze these datasets in a reasonable amount of time are almost non-existent or do not scale well in terms of computer time and memory. These impediments mean that scientists must work with incomplete information and often unrepresentative data to predict how seagrass diversity might change in the future. We discuss these shortfalls and provide a framework for overcoming the impediments and diminishing the knowledge gaps they generate.